Anti‐sickling potential of a Nigerian herbal formula (ajawaron HF) and the major plant component (Cissus populnea L. CPK)

Abstract

The anti-sickling activities of the extracts of the roots of a plant Cissus populnea L. (CPK) (a major constituent of a herbal formula Ajawaron HF used in the management of sickle cell disease in south-west Nigeria) has been examined. Phytochemical examination of the extract showed the presence of anthraquinone derivatives, steroidal glycosides and cardiac glycosides. Alkaloids and tannins were completely absent in the CPK extracts. Evaluation of the anti-sickling activity involved the use of both positive (p-hydroxybenzoic acid, 5 microg/mL) and negative control (normal saline) for each set of experiments aimed at the inhibition of sodium metabisulphite-induced sickling of the HbSS red blood cells obtained from confirmed non-crisis state sickle-cell patients. The chloroform and water partitioned fractions of the cold methanol extracts of CPK exhibited a 62.2% and 52.9% inhibition of sickling, respectively, at 180 min. The herbal formula (HF) aqueous extract showed the highest anti-sickling activity on a weight by weight basis of all the extracts and fractions tested, giving a 71.4% inhibition of sickling at the end of 180 min incubation when compared with the normal saline control. The maximum percentage inhibition of sickling exhibited by the p-hydroxybenzoic acid control was 46.0% at 90 min incubation.

Moringa oleifera Lam. (Moringaceae) grown in Nigeria: In vitro antisickling activity on deoxygenated erythrocyte cells

“…p -Hydroxybenzoic acid (5 mg/ml) was used as a positive control, while normal saline was used as a negative control. The percentage inhibition of sickling was calculated using the formula given in Moody et al .,[19] while the sickling reversal activity of the different plant extracts/fractions was evaluated by using the procedure described by Oduola et al . [20]…”

Structure Activity Relationship (SAR) of Some Benzoic Acid Derivatives from Plant Origin that Exhibit Anti-Sickling Properties in vitro - (Review)

“…3,5-Dimethoxy-4-hydroxybenzoic acid or Protocatechuic acid, p-hydroxy-benzoic acid, vanillic acid, syringic acid and ferulic acid were tested in vitro and their anti-sickling properties were confirmed [8,9]. P-Hydroxy-benzoic acid has also been reported to have antibacterial (against Gram positive and negative bacteria), antifungal, anti-algal, antimutagenic and estrogenic activity [10].…”

Étude de l’activité antifalcemiante et de la thermo- et photo-dégradation des anthocyanes de <i>Centella asiatica, Thomandersia hensii et Maesopsis eminii</i>

“…Dans certaines régions d'Afrique, les porteurs du trait drépanocytaire représentent jusqu'à 20% de la population avec une prévalence en Afrique Centrale de 25 à 30% (Egunyomi et al, 2009 ;Iyamu et al, 2002 ;Moody et al, 2003 ;Ogunyemi et al, 2008;Voet et Voet, 1998). Deux pourcents de la population en République Démocratique du Congo (RDC) sont touchés par cette maladie, soit plus d'un million de personnes (Mpiana et al, 2012).…”