Moringa oleifera Lam. (Moringaceae) grown in Nigeria: In vitro antisickling activity on deoxygenated erythrocyte cells

Abstract

Context:Traditional medicine, which is more available and affordable for the poor uses medicinal plants for the treatment and management of various ailments, including the sickle cell disease (SCD). About 24 million Nigerians are carriers of this sickled cell gene, while approximately 2.4 million are SCD patients. Moringa oleifera Lam. (Moringaceae) possesses high nutritional value and has been used in folklore medicine to treat various ailments related to pain and inflammation. Chemical, pharmacological and pharmacognostical applications of Moringa oleifera have been reported.Objective:This study investigated the antisickling potential of polar and non-polar extracts of the seed, flower and leaf of Moringa oleifera for the first time.Materials and Methods:Using crude methanol extract, aqueous extract, ethyl acetate and butanol, the in vitro antisickling activities of Moringa oleifera fractions, were evaluated using erythrocyte cells deoxygenated with 2% sodium metabisulphite. p-Hydroxybenzoic acid and normal saline were employed as positive and negative controls.Results:Phytochemical screening revealed the presence of saponins, free anthraquinones, and alkaloids. Extracts of the seed and flower demonstrated a higher (P<0.05) antisickling activity in comparison to the leaf extract. The leaf extract, as well as those of the seed and flower, equally demonstrated a (P<0.05) reversal of sickled erythrocytes.Discussions and Conclusions:These findings suggest that Moringa oleifera may play a role in the management of SCD, by incorporation of its fractions into recipes. More extensive biological evaluations and further studies will be necessary for the chemical characterization of the antisickling principles.

Indigenous Knowledge of Medicinal Plants Among Dozo Hunters: An Ethnobotanical Survey in Niamberla Village, Burkina Faso

“…21 Another plant that was cited for treatment of symptoms of sickle cell disease, Moringa oleifera (Toubabou-nèré), showed potential for antisickling properties in a study from Nigeria. 22 These results provide a useful platform for future studies about medicinal plants used by Dozo hunters. As mentioned before, a similar study in the same village and surrounding villages could focus on the frequency of use of the plants noted in this study.…”

Modulatory Role of Moringa Extract and Vitamin E Contra Zinc Oxide Nanoparticles-Induced Nephropathy in Male Albino Rats

“…leaves and pods make it virtually an ideal nutritional supplement. [25] It possesses various biomedical properties such as anti-inflammatory, antioxidant, antimicrobial, antifertility, anticancer, antihepatotoxic and antiulcer activities. [26] VE has a tocopherol structure.…”

IN VITRO ANTIBACTERIAL ACTIVITIES OF DIFFERENT EXTRACT OF Moringa oleifera LEAF ON SOME PATHOGENIC BACTERIA

“…Antibacterial activity of each plant extract was determined by using modified kirby bauer or agar well diffusion method [7]. Broth culture of test bacteria was spread on the Muller Hinton agar Media in duplicates and microbes broth culture was applied on media by swabbing using swab stick under aseptic conditions.…”

“…The plants is claimed to have many pharmaceutical benefits: antibacterial, anticancer, antiasthma, antihypertensive, immunity booster and antinflammatory. It improves fertility and reproductive health and nutritional supplement [6,7]. It is a well-documented world renowned plant herb and also used in water purification and therefore helps in reducing the incidence of water borne diseases [8].…”