Anti-Proliferative Activity and Apoptosis Induction of an Ethanolic Extract of Boesenbergia pandurata (Roxb.) Schlecht. against HeLa and Vero Cell Lines

Abstract

Rhizomes of Boesenbergia pandurata (Roxb.) Schlecht have been reported to contain active compounds with anticancer properties. This research was carried out to examine anti-proliferative and apoptotic induction against HeLa and Vero cells-line. Dried powder of B. pandurata rhizomes was extracted by a maceration method using 90% ethanol. Cytotoxic assays to determine IC 50 and anti-proliferative effects were carried out by MTT methods. Observation of apoptosis was achieved with double staining using acridine orange and ethidium bromide. The results showed that ethanolic extract of B. pandurata was more cytotoxic against HeLa cells (IC 50 of 60 µg/ mL) than Vero cells (IC 50 of 125 µg/mL). The extract had higher anti-proliferative activity as well as apoptotic induction in HeLa than Vero cells. Therefore, it was concluded that the ethanolic extract of B. pandurata had anti-proliferative as well as apoptosis induction activity dependent on the cell type.

Fingerroot (Boesenbergia pandurata) Extract Inhibits Proliferation and Migration of 4T1 Metastatic Breast Cancer Cells

“…This finding indicated that FE had high cytotoxic potency towards 4T1 cells according to Prayong, et al (2008). FE was already known to exhibit high cytotoxic potential in various cells, such as MCF-7 breast cancer cells and HeLa cervical cancer cells (Zaeoung, et al, 2005;Listyawati, et al, 2016). Furthermore, flavonoid compound, in the form of isolates and synthesized from fingerroot, such as panduratin A, boesenbergin A, cardamonin, and pinostrobin, are also known to exhibit anticancer activity towards various cancer cell line (Cheah, et al, 2011;Isa, et al;, Jia, et al, 2016Xian, et al, 2012).…”

Panduratin A: a possible potential treatment for keloids identified by in vitro inhibition of fibroblast migration on scratch wound healing assays

“…9,10 Panduratin A is a phytochemical compound which is known to have potent anti-proliferation, antioxidation, and anti-in ammation effects. [9][10][11] As many compounds with antioxidant, anti-proliferative, and anti-in ammation properties are proposed for anti-keloid treatment, 12 panduratin A could be a potential treatment in keloid patients.…”

“…This results in reduced collagen type 1 deposition, inhibited broblast proliferation, and induction of cell apoptosis. 9,12 PDGF is also a key proin ammatory cytokine that causes keloid formation and is highly expressed in keloid tissue compared to normal tissue. 19 It is responsible for a central signaling pathway in the development of brosis.…”

A Potential Anticancer Mechanism of Finger Root (Boesenbergia rotunda) Extracts against a Breast Cancer Cell Line

“…Previous research mentioned that B. rotunda hexane extract was toxic to liver, lung, and colon cancer cell lines [ 20 ]. Previous studies stated that the ethanol extract of B. rotunda has antiproliferative activity and induces apoptosis in HeLa cervical cancer cells [ 21 ]. The anticancer activity of B. rotunda is predicted because of the presence of their bioactive compounds.…”