Fingerroot (Boesenbergia pandurata) Extract Inhibits Proliferation and Migration of 4T1 Metastatic Breast Cancer Cells

Nurrachma, Marsya Yonna; Maran, Gergorius Gena; Putri, Nindya Budiana; Esti, Yuni Fajar; Hermawan, Adam; Meiyanto, Edy; Jenie, Riris Istighfari

doi:10.14499/indonesianjcanchemoprev11iss3pp103-114

Cited by 2 publications

(1 citation statement)

References 37 publications

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“…Intracellular ROS levels of each cell were detected by the 2',7'-dichlorofluorescin diacetate (DCFDA) assay 16 . Cells were incubated with 25 M DCFDA (Thermo Fisher Scientific, Inc.) in supplemented buffer (10% FBS in phosphate buffer saline) for 30 min at 37℃ in the incubator.…”

Section: Ros Measurement With Dcfda Staining Using Flow Cytometrymentioning

confidence: 99%

The Potential of Moringa Leaf Extract to Prevent Aging Targeted Cellular Senescence

et al. 2023

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High exposure to ultraviolet light and chemicals causes cell senescence due to the overproduction of reactive oxygen species (ROS). Cell senescence is known as one of the causes of degenerative diseases. Reducing ROS levels using antioxidant compounds can overcome cell senescence. This study aimed to evaluate the potential of Moringa leaf extract (EDK) as an anti-aging agent and predict the molecular mechanisms underlying its activity. EDK active compounds were identified through qualitative phytochemical screening, a cytotoxic assay was evaluate using MTT assay, ROS level calculate under DCFDA flow cytometry assay, and cell senescence analyze using SA-β-galactosidase assay. The molecular mechanism that plays an important role in senescence was analyzed through bioinformatics studies and molecular docking in silico to investigate the interaction strength of the active compound EDK with the target protein. The results showed that the EDK ethanol extract contained flavonoid, phenolic, alkaloid, saponin, and steroid compounds. This extract did not give any cytotoxic effect on NIH3T3 cells, as evidenced by the IC50 value of 420μg/mL. EDK concentrations of ¼IC50 (105μg/mL) and ½IC50 (210μg/mL) significantly reduced ROS level in dose-dependent manner in the NIH3T3 cell population with high oxidative stress induced by Doxorubicin 10nM (Dox). The percentage of senescence cells also decreased due to EDK 210μg/mL administration up to 47.60% compared to the positive aging control Dox 10nM (78.90%). Bioinformatics studies found that p21 and TP53 proteins were most directly affected by EDK active compounds in the senescence pathway. The quercetin compound in EDK has the most robust interaction strength against p21 and TP53 proteins compared to ligands. In conclusion, it can be concluded that the active compound EDK can suppress intracellular ROS levels and compete with p21 and TP53 proteins in preventing cell senescence.

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Section: Ros Measurement With Dcfda Staining Using Flow Cytometrymentioning

confidence: 99%

The Potential of Moringa Leaf Extract to Prevent Aging Targeted Cellular Senescence

et al. 2023

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In vitro synergistic effect of hesperidin and doxorubicin downregulates epithelial-mesenchymal transition in highly metastatic breast cancer cells

Amalina

Salsabila

Zulfin

et al. 2023

J Egypt Natl Canc Inst

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Background We previously reported that in highly metastatic breast cancer cells, doxorubicin (DOX) at non-toxic concentrations promoted cell migration and invasion. Hesperidin (30, 5, 9-dihydroxy-40-methoxy-7-orutinosyl flavanone) is a flavonoid glycoside isolated from citrus/lemon plant that possesses a cytotoxic effect in several cancer cells. In this study, we investigate whether DOX efficacy is enhanced by hesperidin (Hsd) and the molecular pathway involved in highly metastatic breast cancer, 4T1. Methods Combined cytotoxicity of Hsd and DOX was evaluated with MTT assay and was analyzed using Chou-Talalay’s method. To better understand the underlying mechanism, several factors, including apoptosis and cell cycle arrest were analyzed by flow cytometry. In addition, antimigration activity was evaluated by scratch wound healing assay, MMP-9 expression by ELISA and gelatin zymography, and Rac-1 protein level using western blot. The data on survival rate and expression level of MMP-9 and Rac-1 were obtained from Gene Expression OMNIBUS (GEO). Results Under MTT assay, Hsd showed a cytotoxic effect in a concentration-dependent manner with an IC50 value of 284 µM on 4T1 cells. Hsd synergistically enhanced the cytotoxic effect of DOX which seemed to correlate with an increase in apoptotic cell death, G2/M cell cycle arrest and blocked the migration of 4T1 cells. At 10 nM, doxorubicin induced lamellipodia formation, and increased the level of Rac-1 and metalloproteinase-9 (MMP-9) expression. Interestingly, combined treatment of DOX and Hsd dramatically downregulated the expression of MMP-9 and Rac-1. These results indicated that Hsd block the cell migration induced by DOX under in vitro studies. Conclusion These findings strongly suggest that Hsd possesses a potential synergistic effect that can be developed to enhance the anticancer efficacy of DOX and reduce the risks of chemotherapy use in highly metastatic breast cancer.

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Fingerroot (Boesenbergia pandurata) Extract Inhibits Proliferation and Migration of 4T1 Metastatic Breast Cancer Cells

Cited by 2 publications

References 37 publications

The Potential of Moringa Leaf Extract to Prevent Aging Targeted Cellular Senescence

The Potential of Moringa Leaf Extract to Prevent Aging Targeted Cellular Senescence

In vitro synergistic effect of hesperidin and doxorubicin downregulates epithelial-mesenchymal transition in highly metastatic breast cancer cells

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