Anti-proliferative Activities of Metallic Nanoparticles in an in Vitro Breast Cancer Model

Abstract

Aims: To investigate effect of metallic nanoparticles, silver (AgNPs) and gold nanoparticles (AuNPs) as antitumor treatment in vitro against human breast cancer cells (MCF-7) and their associated mechanisms. This could provide new class of engineered nanoparticles with desired physicochemical properties and may present newer approaches for therapeutic modalities to breast cancer in women. Materials and Methods: A human breast cancer cell line (MCF-7) was used as a model of cells. Metallic nanoparticles were characterized using UV-visible spectra and transmission electron microscopy (TEM). Cytotoxic effects of metallic nanoparticles on MCF-7 cells were followed by colorimetric SRB cell viability assays, microscopy, and cellular uptake. Nature of cell death was further investigated by DNA analysis and flow cytometry. Results: Treatment of MCF-7 with different concentrations of 5-10nm diameter of AgNPs inhibited cell viability in a dose-dependent manner, with IC50 value of 6.28μM, whereas treatment of MCF-7 with different concentrations of 13-15nm diameter of AuNPs inhibited cell viability in a dose-dependent manner, with IC50 value of 14.48μM. Treatment of cells with a IC50 concentration of AgNPs generated progressive accumulation of cells in the S phase of the cell cycle and prevented entry into the M phase. The treatment of cells with IC50 concentrations of AuNPs similarly generated progressive accumulation of cells in sub-G1 and S phase, and inhibited the entrance of cells into the M phase of the cell cycle. DNA fragmentation, as demonstrated by electrophoresis, indicated induction of apoptosis. Conclusions: Our engineered silver nanoparticles effectively inhibit the proliferation of human breast carcinoma cell line MCF-7 in vitro at high concentration (1000 µM) through apoptotic mechanisms, and may be a beneficial agent against human carcinoma but further detailed study is still needed.

The Synergistic Cytotoxic Effect of Laser-Irradiated Gold Nanoparticles and Sorafenib Against the Growth of a Human Hepatocellular Carcinoma Cell Line

“…Therapeutic options in advanced irresectable HCC are limited to sorafenib, which is the available conventional chemotherapy for HCC treatment. However, the acquired resistance to sorafenib, in addition to its severe side effects and toxicity, limits its beneficial effects (Loutfy et al, 2015;Abdel Hamid et al, 2018;Raoul et al, 2018). Therefore, the current study aimed at minimizing the toxic effects of sorafenib through investigating additive or synergistic potential of photothermal therapy (PTT) by using laser-irradiated gold nanoparticles.…”

Jolkinolide B induces apoptosis and inhibits tumor growth in mouse melanoma B16F10 cells by altering glycolysis

“…The cytotoxicity of the JB against B16F10 cells was tested by Sulforhodamine B (SRB) assay27. In brief, B16F10 cells were collected after digestion with 0.05% Trypsin-EDTA and cultured in 96-well microplates (1 × 10 5  cells/mL, 100 μL).…”

Preparation and cytotoxic activity of resveratrol-gold nanoparticles conjugated to folic acid against MCF-7 cell line

“…This result is reasonable and can be explained in two ways. The first is that metal nanoparticles has been proved to be possess a cytotoxic activity thus can act synergistically with anti-cancer agents [18,19]. Secondly folic acid on FA-AuNp-Rsv conjugate can act as a helper that increase the amount of conjugate presence on the MCF-7 cells surface through binding with folate receptors and by that maximize the drugs that enter the cells.…”