Anti-phospholipid Antibodies in HIV Infection and SLE With or Without Anti-phospholipid Syndrome: Comparisons of Phospholipid Specificity, Avidity and Reactivity with β2-GPI

Petrovas, Constantinos; Vlachoyiannopoulos, Panayiotis G.; Kordossis, T.; Moutsopoulos, Haralampos Μ.

doi:10.1006/jaut.1999.0324

Cited by 93 publications

(82 citation statements)

References 35 publications

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“…19 More recently, aCL in HIV infection were shown to be of the ''infectious'' type, binding being reduced if b 2 GPI was added. Anti-b 2 GPI was detected in only 5% of a series by Petrovas et al, 11 a finding which was confirmed by Gonzales et al…”

Section: Discussionmentioning

confidence: 57%

Anticardiolipin, anti- 2-glycoprotein I and antiprothrombin antibodies in black South African patients with infectious disease

Loizou

Singh²,

Wypkema³

et al. 2003

Annals of the Rheumatic Diseases

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Objectives: To investigate IgG, IgM, and IgA, antiphospholipid antibodies (aPL), against cardiolipin (aCL), b 2 -glycoprotein I (anti-b 2 GPI), and prothrombin (anti-PT), in black South African patients with infectious disease. Unlike patients with systemic lupus erythematosus (SLE) and the antiphospholipid syndrome (APS), raised levels of aPL in infectious diseases are not usually associated with thrombotic complications. Patients and methods: Serum samples from 272 patients with a variety of infectious diseases (100 HIV positive, 112 leprosy, 25 syphilis, 25 malaria, and 10 HCV patients) were studied and compared with autoantibody levels in 100 normal controls. All three aPL were measured using commercial enzyme linked immunosorbent assay (ELISA) kits. Results: Raised levels of all thee aPL were found in all patient groups studied: aCL in 7%, anti-b 2 GPI in 6%, and aPT in 43% of 100 HIV patients, in 29%, 89%, and 21% of 112 patients with leprosy, in 8%, 8%, and 28% of 25 patients with syphilis, in 12%, 8%, and 28% of 25 patients with malaria, and in 20%, 30%, and 30% of 10 HCV patients studied, respectively. Conclusions: The prevalence of aCL and anti-b 2 GPI in black South African HIV positive patients, or those with syphilis, malaria, or hepatitis C virus is lower than reported for mixed race or white populations. aPT were the most prevalent aPL detected in these patient groups, except in patients with leprosy, for whom anti-b 2 GPI was the most prevalent, and where the spectrum of aPL was similar to that seen in patients with SLE and APS.

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Section: Discussionmentioning

confidence: 57%

Anticardiolipin, anti- 2-glycoprotein I and antiprothrombin antibodies in black South African patients with infectious disease

Loizou

Singh²,

Wypkema³

et al. 2003

Annals of the Rheumatic Diseases

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“…This is partly correlated with an increased serum level of antiphospholipid and anticardiolipin antibodies in these patients [13][14][15][16][17]. In a prospective study of 41 pregnant women, Jansen et al [18] showed that HIV-infected pregnant women have 157-fold higher risk of thromboembolism compared with HIV-negative pregnant women and have 120-fold higher risk of thromboembolism compared with HIV-positive men.…”

Section: Introductionmentioning

confidence: 99%

Serum concentrations of antiphospholipid and anticardiolipin antibodies are higher in HIV-infected women

Abdollahi

Morteza

2011

Rheumatol Int

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Manifestations of viral infections like human immune deficiency virus (HIV) differ between women and men. There are some reports due to a higher risk of thrombosis due to antiphospholipid syndrome in HIV-infected women compared with men. The purpose of this study was demonstrating the impact of gender on serum concentration of antiphospholipid and anticardiolipin antibodies in HIV-infected patients. A total of 58 HIV-infected patients and 58 age, sex-matched healthy adults were enrolled. We measured platelets count, hemoglobin, partial thromboplastin time (PTT); prothrombin time (PT), international normalized ratio (INR), antiphospholipid IgG/IgM and anticardiolipin IgG/IgM serotypes in our studied population. Age, male-to-female ratio, hemoglobin level, PT, INR, antiphospholipid IgM isotype, anticardiolipin IgM and IgG isotypes levels were not significantly different between HIV-infected patients and healthy individuals, when CD4+ T-cell count, CD8+ T-cell count, platelet count, and PTT were significantly lower, and antiphospholipid IgG isotype levels was significantly higher in HIV patients. Antiphospholipid IgG isotypes (3.2 [2.8-4.2] vs. 2.5 [2.3-3.1]; P < 0.001) and anticardiolipin IgG isotypes (2.2 [1.9-2.7] vs. 1.4 [1.4-1.9]; P < 0.001) were significantly higher in HIV-infected women than in HIV-infected men. Antiphospholipid IgG/IgM and anticardiolipin IgG/IgM were significantly correlated in HIV-infected men and women when they did not had any correlation in controls. We suggest that HIV infection accelerate thrombosis in women more profoundly than in men, as a result of differences in disease progression and response to therapy in these patients.

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“…Since then, evidence has suggested that ␤2GPI reactivity is related to the pathogenic potential of APLs, leading to the classification of ␤2GPI-dependent autoimmune-and ␤2GPI-independent infection-associated types of APLs (6,7 ).…”

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confidence: 99%

Biosensor Analysis of β2-Glycoprotein I–Reactive Autoantibodies: Evidence for Isotype-Specific Binding and Differentiation of Pathogenic from Infection-Induced Antibodies

et al. 2007

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Background: For the laboratory diagnosis of the antiphospholipid syndrome (APS) we developed a biosensor with the ability to distinguish between diseaserelevant anti-␤2-glycoprotein I (␤2GPI) autoantibodies (anti-␤2GPI) and pathogen-specific ␤2GPI cross-reactive antibodies that occur transiently during infections. Methods: We used a surface plasmon resonance (SPR) biosensor device. For the detection of anti-␤2GPI in serum samples, affinity-purified human ␤2GPI was covalently attached to a functionalized n-alkanethiol self-assembling monolayer on the biosensor chip. After verifying the specificity of the biosensor system with a panel of monoclonal antibodies to ␤2GPI, we analyzed sera from healthy donors and patients suffering from APS, systemic lupus erythematosus (SLE), syphilis, or parvovirus B19 infections. The SPR results were compared with ␤2GPI-specific ELISA. Results: Using the SPR biosensor, we recorded antigen binding curves with response levels in the range of 50 -500, resonance units (RU) for anti-␤2GPI ELISApositive APS patient sera. The amplitudes of the antiphospholipid antibody (APL) responses in the biosensor correlated with the overall IgG and IgM anti-␤2GPI ELISA titers with a correlation coefficient of 0.87. Moreover, we observed immunoglobulin isotype-specific association and dissociation profiles for APL binding of

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Anti-phospholipid Antibodies in HIV Infection and SLE With or Without Anti-phospholipid Syndrome: Comparisons of Phospholipid Specificity, Avidity and Reactivity with β2-GPI

Cited by 93 publications

References 35 publications

Anticardiolipin, anti- 2-glycoprotein I and antiprothrombin antibodies in black South African patients with infectious disease

Anticardiolipin, anti- 2-glycoprotein I and antiprothrombin antibodies in black South African patients with infectious disease

Serum concentrations of antiphospholipid and anticardiolipin antibodies are higher in HIV-infected women

Biosensor Analysis of β2-Glycoprotein I–Reactive Autoantibodies: Evidence for Isotype-Specific Binding and Differentiation of Pathogenic from Infection-Induced Antibodies

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