Anti-Microbial Activity and Spectro-Chemical Investigation of Ink Extracts of <i> Sepiella inermis</i> (Van Hasselt 1835)

Abstract

The crude petroleum ether and methanol ink extracts of Sepiella inermis were tested for their antimicrobial activity against human pathogenic fungi and bacteria by disc diffusion method. Spectral analysis was carried out by UV-VIS spectrophotometer, FT-IR, Raman IR and GC-MS. Of the two solvent extracts, only methanol extract was active and no activity was detected in petroleum ether extract. The human pathogenic fungus Candida albicans and bacterium Proteus vulgaris were found to be highly sensitive, with an inhibition zone of 20 and 19 mm respectively. GC-MS of methanol ink extract revealed sixteen compounds belonging to the derivatives of dihydroxy indole-2-carboxylic acid and dihydroxyindole. These investigations proved that methanol ink extract of Sepiella inermis possess significant antimicrobial property against both fungus and gram -ve bacteria. Since ink of sepia is available abundantly as a waste material, studies focused on isolation and characterization of bioactive substances pave the way for new antimicrobial compounds.

Antibacterial Activity of Melanin from Cuttlefish and Squid Ink

“…Pertumbuhan memasuki fase stasioner setelah jam ke-12 hingga jam ke-24. Vasantharaja et al (2014) melaporkan bahwa ekstrak metanol tinta Sepiella inermis dengan menggunakan GC-MS menunjukkan adanya campuran dari struktur oligomer yang merupakan gabungan antara dihidroksi indol-2-asam karboksilat dan dihidroksiindol. Ekstrak metanol ini memiliki aktivitas penghambatan terutama terhadap bakteri Gram negatif misalnya Proteus vulgaris, Pseudomonas aeruginosa dan E. coli.…”

Antibacterial Activity of Cuttlefish Sepia sp. (Cephalopoda,) Ink Extract Against Aeromonas hydrophila

“…However, the melanin concentration of cuttlefish ink extract (0.010 g / mL) was able to kill bacteria so that after 24-hour incubation, there was only one living cell (inhibition reached 99.99%) (Fitrial and Khotimah, 2017). Besides, Vasantharaja et al (2014) reported that methanol extracts of Sepia sp. ink using GC-MS showed a mixture of oligomeric structures, which was a combination of dihydroxy indol-2-carboxylic acid and dihydroxyindole.…”