Antibacterial Activity of Cuttlefish Sepia sp. (Cephalopoda,) Ink Extract Against Aeromonas hydrophila

Abstract

Cephalopods ink has shown potential antiretroviral activity. The ink extracts of cuttlefish showed an antibacterial effect. This study aims to investigate the antibacterial activity of the methanolic extract of the ink of cuttlefish Sepia sp. against Aeromonas hydrophila. A. hydrophila are opportunists and associated with aquatic fish and shrimp disease. The shade-dried ink sample from approximately 30g ink sacs obtained from 15 animals was immersed separately in methanol (1:3 w/v) solvents for overnight. The experiment in this study used the dried extract of cuttlefish ink. The isolate of A. hydrophila was originated from Jepara Brackishwater Aquaculture Center. The average yield percentage of cuttlefish ink extract obtained was 4.86%. The results of the MIC test in Table V show that the highest average absorbance value obtained was at a concentration of 50 ppm, which was equal to 1.716 nm, and the lowest absorbance was obtained at a treatment dose of 300 ppm at 0.841 nm, while the Mc Farland tube was 0.933 nm. The results of antibacterial test on Table II showed antibacterial activity of cuttlefish ink extract at negative concentration control showed diameter zone of 5 ± 1.2 mm, at positive control showed diameter zone of 31 ± 1.2 mm, at 250 ppm result 19 ± 0.9 mm, at 300 ppm result 22 ± 1.4 mm, at 350 ppm result 31 ± 1.2 mm.

Eco-friendly strategy for producing bio-based silver nanoparticles (AgNPs) employing Sepioteuthis lessoniana ink, in addition to biological and degradation of dye applications

“…The bacterial strains (Escherichia coli, Klebsiella pneumonia, Streptococcus pneumonia, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus) in the freshly prepared subculture were used for antibacterial activity by the agar disc diffusion method [13]. Various concentrations of squid ink silver nanoparticles (25,50,75, and 100 µg/ml) were used for this activity.…”

“…The synthesis of SI-AgNP was carried out using a biological process similar to that of Ray et al [13] with minor modi cations. The silver nitrate solution was prepared at 0.1 mM in 100 ml of distilled water.…”

“…Reduction of AgNPs in the solution was monitored and evaluated by using UV-Visible spectroscopy on aliquots (1 ml) of the aqueous component. The spectrum of these aliquots was investigated, as well as the response of the silver nanoparticles, with a UV-Visible spectrophotometer (Shimadzu UV-1700 Spectrometer) with a resolution of 1 nm from 200 to 800 nm was taken [13].…”

Characteristics of Tuna Viscera (Thunnus sp.) Hydrolysate Protein Fermented by Bacillus licheniformis

“…Preparation of bacteria conducted according to the published method (Islamy, 2019). The inoculum (pure culture) used is the bacterium B. licherniformis B1008 (collection of the Microbiology Laboratory LIPI Cibinong).…”

Potential the Black Soldier Fly (Hermetia illucens) in Feed Formulation for Growth of Common Carp (Cyprinus carpio L.)

“…Besides that, insect cultivation can reduce KEYWORDS Black soldier fly; Cultivation media; Protein source; Substitution, Common carp. organic waste, which has the potential to pollute the surrounding environment (Li et al, 2011) where the waste pollution can lead to a negative impact on the environment (Islamy et al, 2017;Kilawati and Islamy, 2019) and develop a negative microorganism (Islamy, 2019). Another advantageous factor is that insect-based protein sources do not compete with humans, so it is suitable for animal feed ingredients, including poultry and fish (Veldkamp et al, 2012).…”