Anti-inflammatory Steroid from Phragmitis rhizoma Modulates LPS-Mediated Signaling Through Inhibition of NF-κB Pathway

Park, Sook Jahr; Kim, Young Woo; Park, Moon Ki; Byun, Sung Hui; Kim, Sang Chan; Lee, Jong Rok

doi:10.1007/s10753-015-0299-6

Cited by 27 publications

(19 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, stigmasta‐3, 5‐dien‐7‐one and vanillin were probably responsible for the resistance to fungal attack. The former is known to possess anti‐inflammatory activity . The latter has since long been used in certain medical applications and as a flavoring agent, because of its inhibitory properties against fungi and bacteria and synergistic effect to enhance the antifungal effect of plant essential oils against Botrytis cinerea .…”

Section: Resultsmentioning

confidence: 99%

Effects of biocide treatments on durability of wood and bamboo/high density polyethylene composites against algal and fungal decay

Feng

Chen

et al. 2017

J of Applied Polymer Sci

View full text Add to dashboard Cite

The main objective of this study was to investigate the algal and fungal resistance of biocide‐treated wood flour (WF)/high density polyethylene (HDPE) and bamboo flour (BF)/HDPE composites. The biocides included 4,5‐dichloro‐2‐octyl‐isothiazolone (DCOIT), zinc pyrithione (ZPT), and carbendazim (MBC). Resistance to algae and fungi was evaluated by artificially accelerated tests. Treated and untreated samples were exposed to algae (Chlorella vulgaris, Ulothrix sp., Scenedesmus quadricauda, and Oscillatoria sp.) and fungi (Coriolus versicolor and Poria placenta) for 21 days and 12 weeks, respectively. The volatile components of WF and BF extractives were analyzed by gas chromatography‐mass spectrometry (GC‐MS). The results indicated that incorporation of DCOIT, ZPT, and MBC effectively enhanced the durability of WF/HDPE and BF/HDPE composites against algal and fungal decay. Accordingly, DCOIT, ZPT, and MBC can be used as potential biocides for both WF/HDPE and BF/HDPE composites. GC‐MS analysis suggested that palmitic acid, oleic acid, stigmasta‐3,5‐dien‐7‐one, and vanillin in WF possibly provided some resistance to fungal attack, whereas di (2‐ethylhexyl) phthalate and linoleic acid in BF were responsible for algal resistance. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45148.

show abstract

Section: Resultsmentioning

confidence: 99%

Effects of biocide treatments on durability of wood and bamboo/high density polyethylene composites against algal and fungal decay

Feng

Chen

et al. 2017

J of Applied Polymer Sci

View full text Add to dashboard Cite

show abstract

“…HO-1 is known as a representative antioxidant enzyme which plays an important role in protecting cells from oxidative stress and is regulated by the transcriptional activity of nuclear factor erythroid 2-related factor 2 (Nrf2) [ 53 , 54 ]. Therefore, we investigated whether phenylheptatriyne ( 1 ) expresses the antioxidant enzyme HO-1, and whether the expression of HO-1 was increased ( Figure 7 ).…”

Section: Discussionmentioning

confidence: 99%

Anti-Neuroinflammatory and Anti-Inflammatory Activities of Phenylheptatriyne Isolated from the Flowers of Coreopsis lanceolata L. via NF-κB Inhibition and HO-1 Expression in BV2 and RAW264.7 Cells

Lee

Liu

Yoon

et al. 2021

IJMS

View full text Add to dashboard Cite

Aging is associated with immune disregulation and oxidative stress which lead to inflammation and neurodegenerative diseases. We have tried to identify the anti-neuroinflammatory and anti-inflammatory components of Coreopsis lanceolata L. The dried flowers of C. lanceolata were extracted with 70% EtOH, and the obtained extract was divided into CH2Cl2, EtOAc, n-BuOH, and H2O fractions. The CH2Cl2 fraction was separated using silica gel and C-18 column chromatography to yield phenylheptatriyne (1), 2′-hydroxy-3,4,4′-trimethoxychalcone (2), and 4′,7-dimethoxyflavanone (3). Additionally, the EtOAc fraction was subjected to silica gel, C-18, and Sephadex LH-20 column chromatography to yield 8-methoxybutin (4) and leptosidin (5). All the compounds isolated from C. lanceolata inhibited the production of nitric oxide (NO) in LPS-induced BV2 and RAW264.7 cells. In addition, phenylheptatriyne and 4′,7-dimethoxyflavanone reduced the secretion of inflammatory cytokines, tumor necrosis factor alpha (TNF-α), and interleukin (IL)-6. Among them, phenylheptatriyne was significantly downregulated in the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). Subsequently, phenylheptatriyne also effectively inhibited nuclear factor-kappa B (NF-κB) activation in LPS-stimulated BV2 and RAW264.7 cells. Based on these results, the anti-neuroinflammatory effect of phenylheptatriyne isolated from C. lanceolata was confirmed, which may exert a therapeutic effect in treatment of neuroinflammation-related diseases.

show abstract

“…Previous studies demonstrated that corticosteroids like Dex phosphate contributed to an anti-inflammatory effect through inhibiting the nuclear factor-kappa B (NF-κB) signal pathway. [5, 6] However, whether corticosteroids eye drops are proper for infectious keratitis cure remains a controversial topic. [7] Lu et al proved that dexamethone could inhibit IL-1-induced collagen degradation and decrease stroma scarring.…”

Section: Introductionmentioning

confidence: 99%

Corticosteroids effects on LPS-induced rat inflammatory keratocyte cell model

et al. 2017

View full text Add to dashboard Cite

PurposeCorticosteroids are efficient anti-inflammation treatments. However, there are still arguments on whether it should be used in keratitis. This study was to observe the effect of corticosteroids on keratocytes both in normal condition and inflammation status in vitro.MethodsRat keratocytes were cultured and used for examination. 10 μg/ml lipopolysaccharide (LPS) was used to establish the inflammatory keratocyte cell model, and prednisolone acetate (PA), dexamethasone (Dex) and fluorometholone (Flu) were used as corticosteroids treatments. 5 d-growth curve and cell viabilities were assayed by CCK8, and cell morphologies and migration rate were studied. TNF-α, IL-6 and IL-1β levels were examined by ELISA. Western blotting was used to quantified type VI collagen (Col VI) and matrix metalloproteinase 9 (MMP9) expressions, and immunofluorescence staining assays of Col I and Col VI were carried out.ResultsIn normal condition, proliferation and migration of keratocytes were slightly influenced in PA, Dex and Flu groups. The secretion of Col I and Col VI was suppressed and MMP9 expression increased in corticosteroids groups. But no significant difference was seen in TNF-α, IL-6 and IL-1β expression levels. In inflammatory status, TNF-α, IL-6 and MMP9 levels increased in LPS group, while they significantly decreased in corticosteroids groups. Although keratocytes viabilities and migration were slightly affected in 24 h, no significant differences were seen between LPS group and corticosteroids groups in 5-d proliferation. Col I and Col VI secretion in LPS-keratocytes was maintained with corticosteroids treatments.ConclusionsCorticosteroids showed lightly effects on keratocytes proliferation and migration, but it successfully decreased TNF-α, IL-6 level and maintained the secretion of and Col I and Col VI, while suppressed the expression of MMP9 in LPS-induced keratocytes. PA was suggested to use in early stage of keratitis clinical treatment.

show abstract

Anti-inflammatory Steroid from Phragmitis rhizoma Modulates LPS-Mediated Signaling Through Inhibition of NF-κB Pathway

Cited by 27 publications

References 29 publications

Effects of biocide treatments on durability of wood and bamboo/high density polyethylene composites against algal and fungal decay

Effects of biocide treatments on durability of wood and bamboo/high density polyethylene composites against algal and fungal decay

Anti-Neuroinflammatory and Anti-Inflammatory Activities of Phenylheptatriyne Isolated from the Flowers of Coreopsis lanceolata L. via NF-κB Inhibition and HO-1 Expression in BV2 and RAW264.7 Cells

Corticosteroids effects on LPS-induced rat inflammatory keratocyte cell model

Contact Info

Product

Resources

About