Anti-Neuroinflammatory and Anti-Inflammatory Activities of Phenylheptatriyne Isolated from the Flowers of Coreopsis lanceolata L. via NF-κB Inhibition and HO-1 Expression in BV2 and RAW264.7 Cells

Lee, Hwan; Liu, Zhiming; Yoon, Chi–Su; Dong, Linsha; Ko, Wonmin; Woo, Eun‐Rhan; Lee, Dong-Sung

doi:10.3390/ijms22147482

Cited by 7 publications

(10 citation statements)

References 55 publications

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“…As we known, nuclear factor kappa-B (NF-κB) is a hub for pro-inflammatory transcription factors that induce the expression of many pro-inflammatory genes, such as cell adhesion molecules, cyclooxygenase 2 (COX-2), inducible nitric oxide synthase (iNOS), tumor necrosis factor α (TNF-α), nuclear factor-kappa B (NF-kappaB), and interleukin (IL) ( 31 – 33 ). When endotoxin interacts with Toll-like receptor 4 (TLR4), TNF-α interacts with tumor necrosis factor receptor (TNFR), it will activate the κB kinase (IKK) complex of MAPK/EKK kinase, promote the dissociation of phosphorylated NK-beta inhibitors (IKBα) from the IKBα/NF-κB complex and produce NF-κB monomers, which translocate into the nucleus and produce pro-inflammatory factors, such as TNF-α, IL-1β, IL-6, and IFN-γ) and inflammatory markers (iNOS and COX-2) ( 34 , 35 ).…”

Section: Inflammatory Bowel Disease and Bowel Cancer Seriously Damage...mentioning

confidence: 99%

Probiotics Interact With Lipids Metabolism and Affect Gut Health

Wang

Xiong

et al. 2022

Front. Nutr.

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Probiotics have attracted much attention due to their ability to modulate host intestinal microbe, participate in nutrient metabolism or immunomodulatory. Both inflammatory bowel disease (IBD) and bowel cancer are digestive system disease, which have become a global public health problem due to their unclear etiology, difficult to cure, and repeated attacks. Disturbed gut microbiota and abnormal lipid metabolism would increase the risk of intestinal inflammation. However, the link between lipid metabolism, probiotics, and IBD is unclear. In this review, we found that different lipids and their derivatives have different effects on IBD and gut microbes. ω-3 polyunsaturated fatty acids (PUFAs) docosahexaenoic acid, eicosapentaenoic acid, and their derivatives resolvin E1, resolvin D can inhibit oxidative stress and reactive oxygen species activate NFκB and MAPk pathway. While ω-6 PUFAs linoleic acid and arachidonic acid can be derived into leukotrienes and prostaglandins, which will aggravate IBD. Cholesterol can be converted into bile acids to promote lipid absorption and affect microbial survival and colonization. At the same time, it is affected by microbial bile salt hydrolase to regulate blood lipids. Low denstiy lipoprotein (LDL) is easily converted into oxidized LDL, thereby promoting inflammation, while high denstiy lipoprotein (HDL) has the opposite effect. Probiotics compete with intestinal microorganisms for nutrients or ecological sites and thus affect the structure of intestinal microbiota. Moreover, microbial short chain fatty acids, bile salt hydrolase, superoxide dismutase, glutathione, etc. can affect lipid metabolism and IBD. In conclusion, probiotics are directly or indirectly involved in lipids metabolism and their impact on IBD, which provides the possibility to explore the role of probiotics in improving gut health.

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Section: Inflammatory Bowel Disease and Bowel Cancer Seriously Damage...mentioning

confidence: 99%

Probiotics Interact With Lipids Metabolism and Affect Gut Health

Wang

Xiong

et al. 2022

Front. Nutr.

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“…Methods for measuring IL-6 and TNF-α were performed as previously described using kits commercially available from Bio-Legend (San Diego, CA, USA) [53]. Compounds 16, 17 and 18 isolated from L. erythrocarpa were treated at a concentration of 40 µM, and the supernatant of each well in which an inflammatory reaction occurred was analyzed at a wavelength of 450 nm.…”

Section: Il-6 and Tnf-α Assaymentioning

confidence: 99%

“…The expression levels of the pro-inflammatory proteins iNOS and COX-2 were performed as described above [53]. Cell lysis was lysed with Tris-HCl buffer (20 mM, pH 7.4) containing the protease inhibitor mixture (5 mg/mL pepstatin A, 5 mg/mL aprotinin, 1 mg/mL chymostatin and 0.1 mM phenylmethanesulfonyl fluoride).…”

Section: Western Blot Analysismentioning

confidence: 99%

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Effects of Compounds Isolated from Lindera erythrocarpa on Anti-Inflammatory and Anti-Neuroinflammatory Action in BV2 Microglia and RAW264.7 Macrophage

Yoon

Lee

Liu

et al. 2022

IJMS

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Lindera erythrocarpa contains various constituents such as cyclopentenedione-, flavonoid-, and chalcone-type components. In this study, a novel bi-linderone derivative and 17 known compounds were isolated from the leaves of L. erythrocarpa by using various chromatographic methods. The structures of the components were determined from nuclear magnetic resonance and mass spectrometry data. All isolated compounds were tested for anti-inflammatory and anti-neuroinflammatory activities in lipopolysaccharide (LPS)-induced BV2 and RAW264.7 cells. Some of these compounds showed anti-inflammatory effects by inhibiting the nitric oxide (NO) produced by LPS. In particular, linderaspirone A (16), bi-linderone (17) and novel compound demethoxy-bi-linderone (18) showed significant inhibitory effects on the production of prostaglandin E2 (PGE2), tumor necrosis factor-α, and interleukin-6. The three compounds also inhibited the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), which are pro-inflammatory proteins, and the activation of nuclear factor κB (NF-κB). Therefore, linderaspirone A (16), bi-linderone (17), and demethoxy-bi-linderone (18) isolated from the leaves of L. erythrocarpa have therapeutic potential in neuroinflammatory diseases.

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“…Briefly, the concentration of nitrite in the conditioned media was determined using a method based on the Griess reaction [53]. The details of the assay have been described previously [54].…”

Section: Measurement Of No Productionmentioning

confidence: 99%

Kuwanon T and Sanggenon a Isolated from Morus alba Exert Anti-Inflammatory Effects by Regulating NF-κB and HO-1/Nrf2 Signaling Pathways in BV2 and RAW264.7 Cells

Liu

Kim

et al. 2021

Molecules

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We previously investigated the methanolic extract of Morus alba bark and characterized 11 compounds from the extract: kuwanon G (1), kuwanon E (2), kuwanon T (3), sanggenon A (4), sanggenon M (5), sanggenol A (6), mulberofuran B (7), mulberofuran G (8), moracin M (9), moracin O (10), and norartocarpanone (11). Herein, we investigated the anti-inflammatory effects of these compounds on microglial cells (BV2) and macrophages (RAW264.7). Among them, 3 and 4 markedly inhibited the lipopolysaccharide (LPS)-induced production of nitric oxide in these cells, suggesting the anti-inflammatory properties of these two compounds. These compounds inhibited the production of prostaglandin E2, interleukin-6, and tumor necrosis factor-α, and the expression of inducible nitric oxide synthase and cyclooxygenase-2 following LPS stimulation. Pretreatment with 3 and 4 inhibited the activation of the nuclear factor kappa B signaling pathway in both cell types. The compounds also induced the expression of heme oxygenase (HO)-1 through the activation of nuclear factor erythroid 2-related factor 2. Suppressing the activity of HO-1 reversed the anti-inflammatory effects caused by pretreatment with 3 and 4, suggesting that the anti-inflammatory effects were regulated by HO-1. Taken together, 3 and 4 are potential candidates for developing therapeutic and preventive agents for inflammatory diseases.

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Anti-Neuroinflammatory and Anti-Inflammatory Activities of Phenylheptatriyne Isolated from the Flowers of Coreopsis lanceolata L. via NF-κB Inhibition and HO-1 Expression in BV2 and RAW264.7 Cells

Cited by 7 publications

References 55 publications

Probiotics Interact With Lipids Metabolism and Affect Gut Health

Probiotics Interact With Lipids Metabolism and Affect Gut Health

Effects of Compounds Isolated from Lindera erythrocarpa on Anti-Inflammatory and Anti-Neuroinflammatory Action in BV2 Microglia and RAW264.7 Macrophage

Kuwanon T and Sanggenon a Isolated from Morus alba Exert Anti-Inflammatory Effects by Regulating NF-κB and HO-1/Nrf2 Signaling Pathways in BV2 and RAW264.7 Cells

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