Anti-Inflammatory Potential of Cultured Ginseng Roots Extract in Lipopolysaccharide-Stimulated Mouse Macrophages and Adipocytes

Park, Hyun Ju; Jo, Sang-Mi; Seo, Seok Hee; Lee, Myoungsook; Lee, Yunkyoung; Kang, Inhae

doi:10.3390/ijerph17134716

Cited by 12 publications

(9 citation statements)

References 43 publications

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“…The ginger powder was extracted using the pressurized hot water extraction method (modified from [ 25 ]) as previously described. The final stock concentration of ginger was prepared at 100 mg/mL with several aliquots for in vitro experiments.…”

Section: Methodsmentioning

confidence: 99%

Ginger (Zingiber officinale) Attenuates Obesity and Adipose Tissue Remodeling in High-Fat Diet-Fed C57BL/6 Mice

Seo

Fang

Kang

2021

IJERPH

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Obesity is characterized by excessive fat accumulation in adipose tissue, which is an active endocrine organ regulating energy metabolism. Ginger (Zingiber officinale) is known to have antioxidant, anti-inflammatory, and antiobesity effects, but the role of ginger in modulating adipocyte metabolism is largely unknown. In this study, we hypothesized that ginger supplementation inhibits high-fat (HF)-diet-mediated obesity. C57BL/6 male mice were randomly assigned to three diets for 7 weeks: low fat (LF, 16% kcal from fat), HF (HF, 60% kcal from fat), or HF with 5% ginger powder in diet (HF + G). The HF diet increased body weight (BW) and BW gain, as well as fasting glucose, total cholesterol, and hepatic lipid levels, compared to the LF diet-fed group. Ginger supplementation significantly improved HF-diet-induced BW gain, hyperglycemia, hypercholesterolemia, and hepatic steatosis without altering food intake. Next, we investigated whether ginger modulates adipocyte remodeling. HF-mediated adipocyte hypertrophy with increased lipogenic levels was significantly improved by ginger supplementation. Furthermore, the HF+G group showed high levels of the fatty-acid oxidation gene, carnitine palmitoyltransferase 1 (CPT1), which was accompanied by a reduction in adipocyte inflammatory gene expression. Taken together, our work demonstrated that ginger supplementation attenuated HF-diet-mediated obesity and adipocyte remodeling in C57BL/6 mice.

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Section: Methodsmentioning

confidence: 99%

Ginger (Zingiber officinale) Attenuates Obesity and Adipose Tissue Remodeling in High-Fat Diet-Fed C57BL/6 Mice

Seo

Fang

Kang

2021

IJERPH

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“…LPS interacts with macrophages since they are Toll-like receptor 4 (TLR4)-expressing host cells. The TLR4 activation produces a dysregulation of pro-inflammatory cytokines, including IL-6 and TNF-α, and ROS production [ 19 , 31 ].…”

Section: Resultsmentioning

confidence: 99%

Liposomes Containing Amaranth Unsaponifiable Matter and Soybean Lunasin Suppress ROS Production in Fibroblasts and Reduced Interleukin Production in Macrophages

Dávila-Ortíz

Castañeda-Reyes

Juárez-Palomo

et al. 2022

IJERPH

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Inflammation is a normal response in defense to agents that may cause damage to the human body. When inflammation becomes chronic, reactive oxygen species (ROS) are produced; which could lead to diseases such as cancer. The aim was to assess liposomes’ antioxidant and anti-inflammatory capacity loaded with amaranth unsaponifiable matter and soybean lunasin (UM + LunLip) in an in vitro model using fibroblasts and macrophages. To evaluate ROS production, fibroblasts CHON-002 ABAP were added to promote ROS production; and the cells were treated with UM + LunLip. For inflammation markers production, lipopolysaccharides (LPS)-stimulated RAW 264.7 and peritoneal macrophages were treated with empty liposomes (EmLip), liposomes loaded with unsaponifiable matter (UMLip), liposomes loaded with lunasin (LunLip), and UM + LunLip. ROS production was significantly decreased by 77% (p < 0.05) when fibroblasts were treated with UM + LunLip at 2 mg lunasin/mL compared with the control treated with ABAP. Treatment with UMLip was the most effective in reducing tumor necrosis factor-α (71–90%) and interleukin-6 (43–55%, p < 0.001). Both liposomes containing unsaponifiable matter (UMLip and UM + LunLip) were more effective than EmLip or LunLip. In conclusion, amaranth unsaponifiable matter-loaded liposomes are effective in decreasing pro-inflammatory cytokine production.

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“…LPS cause an oxidative stress starts from weak inflammation to cell destruction (Cheng et al 2018). It releases many pro-inflammatory cytokines such as IL-1 beta, IL-6 and TNF (Park et al 2020) that cause oxidative stress for different types of cells. OEO modulate bacterial, viral and fungal harmful effect in accordance with our results (Puškárová1 et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Oregano-oil antagonist lipopolysaccharide (LPS) induced toxicity in pre- and post-hatch chick embryo

Bekhet

Sayed

2021

Journal of Applied Animal Research

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Oregano essential oil (OEO), one kind of Labiata has been known for a long time as a popular remedy. The essential oil of oregano has anti-bacterial, anti-oxidant, anti-fungal and nematicidal properties mainly due to the basic constituents. Once the shell's microbial barriers have been breached, Gram-negative bacteria are more capable of withstanding the antimicrobials present in the albumen. Eggs were divided into, control group (GI), LPS group (GII) and LPS plus OEO 0.5% group (GIII), as natural disinfectants. Statistical data were analysed, histopathological, RT-PCR and in situ hybridization studies were performed to explain the OEO modulatory role. Lipopolysaccharide (LPS) group represented the highest (P < 0.05) increase of embryonic mortality percentage, greatest significance (P < 0.01) egg weight loss, a decrease of hatchability and high teratogenic effect compared with those for other groups in all types of experiments. Chicks produced from egg subjected to oregano 0.5% and LPS significantly (P < 0.05) recorded large body weight gain, enhancement of hatchability and produces no teratogenic effects in the developing chicken. OEO modulates the effect of LPS in histopathological, RT-PCR and in situ hybridization studies throughout modulation of the Daam2 and Tbx2 gene expression towards the normal one. Using Oregano oil as a natural disinfectant in this study modulate significantly the effect of LPS oxidative effect on the chick embryo even after hatching, so, we conclude that OEO is an effective anti-oxidant that recommended to be used in hatchery for good quality chicks, chick growth and finally improvement of laying production performance.

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Anti-Inflammatory Potential of Cultured Ginseng Roots Extract in Lipopolysaccharide-Stimulated Mouse Macrophages and Adipocytes

Cited by 12 publications

References 43 publications

Ginger (Zingiber officinale) Attenuates Obesity and Adipose Tissue Remodeling in High-Fat Diet-Fed C57BL/6 Mice

Ginger (Zingiber officinale) Attenuates Obesity and Adipose Tissue Remodeling in High-Fat Diet-Fed C57BL/6 Mice

Liposomes Containing Amaranth Unsaponifiable Matter and Soybean Lunasin Suppress ROS Production in Fibroblasts and Reduced Interleukin Production in Macrophages

Oregano-oil antagonist lipopolysaccharide (LPS) induced toxicity in pre- and post-hatch chick embryo

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