Anti-inflammatory efficiency of Ankaferd blood stopper in experimental distal colitis model

Abstract

Background/Aim:Ankaferd blood stopper (ABS) is a herbal extract that enhances mucosal healing. In this study, we aimed to investigate the efficiency of ABS in the treatment of experimental distal colitis.Materials and Methods:Twenty one male albino rats were divided into three groups: Sham control (Group 1), colitis induced by acetic acid and treated with saline (Group 2), colitis induced by acetic acid and treated with ABS (Group 3). At end of the 7th day of induction, all the rats were lightly anesthetized with intramuscular ketamine (8 mg/kg) and thereafter laparotomy and total colectomy were performed. The distal colon segment was assessed macroscopically and microscopically. In addition malondialdehyde (MDA), superoxide dismutase (SOD) and nitric oxide (NO) levels of the colonic tissue and changes in body weight were measured.Results:The MDA and NO levels of the colonic tissues and weight loss were significantly higher in Group 2 compared to Group 1 and Group 3. Microscopic and macroscopic damage scores were significantly higher in Group 2 and Group 3 than Group 1 (P: 0.001, P: 0.004, respectively). Although the microscopic and macroscopic damage scores in Group 3 were slightly lower than Group 2, the difference was not statistically significant. The SOD levels of the colonic tissues were not different between the three groups.Conclusion:Weight alterations and high-levels of the colonic tissue MDA and NO suggested that ABS might have anti-inflammatory effects on experimental distal colitis. However, this suggestion was not supported by histopathological findings.

Effect of Ankaferd Blood Stopper on Skin Superoxide Dismutase and Catalase Activities in Warfarin-Treated Rats

“…41 Kocak et al suggested that ABS had an antioxidant action in the intestine in an experimental distal colitis model which could be attributed to herbal mixtures including G glabra and T. Vulgaris. 42 Consistent with these results in our study, ABS treatment significantly increased SOD activity in the warfarin group at the end of the eighth day when compared to the no hemostatic agent applied group. The SOD is an enzyme that repairs cells and reduces the damage done to them by superoxide, the most common free radical in the body.…”

Assessment of the efficacy of Ankaferd blood stopper on the prevention of postoperative pericardial adhesions : cardiovascular topic

“…Besides its homeostatic activity, Kocak et al 29 reported that Ankaferd might also have anti-inflammatory effects 7. Tests have demonstrated its safety, efficacy, sterility and non-toxicity for external usage 8,30…”

An immunohistochemistry and histopathological study of ankaferd blood stopper in a rat model of cervical inflammation

“…6,7 Besides from these hemostatic properties, the anti-inflammatory effect of ABS are reported to be based on the effect of proinflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and its antioxidant potential. [8][9][10] Difficult to cure in nature, inflammation of the cervix can be treated with topical application of ABS based on its anti-inflammatory effect, which was proved in several studies. 10,11 Unfortunately, to our knowledge, no study in the literature evaluates the beneficial effect of ABS explicitly on inflammatory response during cervicitis.…”

“…[8][9][10] Difficult to cure in nature, inflammation of the cervix can be treated with topical application of ABS based on its anti-inflammatory effect, which was proved in several studies. 10,11 Unfortunately, to our knowledge, no study in the literature evaluates the beneficial effect of ABS explicitly on inflammatory response during cervicitis. Therefore, in this study, we attempted to define and characterize the possible role of ABS in the elimination of a chronic inflammatory process in a rat model of cervicitis.…”