Anti-inflammatory effects of chlorogenic acid in lipopolysaccharide-stimulated RAW 264.7 cells

Hwang, Su Jung; Kim, Yong‐Wan; Park, Yohan; Hj, Lee; Kim, Kyu‐Won

doi:10.1007/s00011-013-0674-4

Cited by 361 publications

(205 citation statements)

References 32 publications

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“…Chlorogenic acid was shown to decrease the levels of COX-2 and PGE2 and was also found to inhibit JNK, but not p38 and ERK (p44/42) phosphorylations on LPS induced Raw 264.7 macrophages [57]. Besides chlorogenic acid was found to inhibit the mRNA expressions of COX-2 and iNOS, and also decreased the levels of pro-inflammatory (IL-1β, TNFα, IL-6) cytokines [58]. Although it was reported that pretreatment of Raw 264.7 macrophage cells with chlorogenic acid up to 37.5 µg/mL concentration did not significantly affect iNOS and NO levels when the cells were treated with LPS for 10 to 12 hours, another study revealed that significant inhibitory effect is attainable at relatively lower concentrations such as 20 µM when cells were treated for 24 hrs [57,58].…”

Section: Structure Elucidation Of the Active Componentsmentioning

confidence: 93%

“…Besides chlorogenic acid was found to inhibit the mRNA expressions of COX-2 and iNOS, and also decreased the levels of pro-inflammatory (IL-1β, TNFα, IL-6) cytokines [58]. Although it was reported that pretreatment of Raw 264.7 macrophage cells with chlorogenic acid up to 37.5 µg/mL concentration did not significantly affect iNOS and NO levels when the cells were treated with LPS for 10 to 12 hours, another study revealed that significant inhibitory effect is attainable at relatively lower concentrations such as 20 µM when cells were treated for 24 hrs [57,58]. In the present study, CA exerted significant NO inhibitory activity at higher concentrations (100 µg/mL) without inducing notable toxicity to cells.…”

Section: Structure Elucidation Of the Active Componentsmentioning

confidence: 97%

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Evaluation of the in vitro Anti-inflammatory Activity of Nerium oleander L. Flower Extracts and Activity-Guided Isolation of the Active Constituents

Balkan¹,

Gören²,

Kırmızıbekmez³

et al. 2018

Rec.Nat.Prod.

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Abstract:The in vitro anti-inflammatory activity profile of the Nerium oleander flower EtOH extract/its subextracts (n-hexane, CH2Cl2, EtOAc, remaining H2O) were evaluated on LPS induced Raw 264.7 macrophages. The effects of the crude EtOH extract and its subextracts on nitric oxide (NO) production and cell viability were determined. The most active subextract was determined to be the EtOAc subextract without exerting any toxicity towards Raw 264.7 macrophages. This subextract significantly inhibited NO production of Raw 264.7 macrophages after LPS induction (62.56±1.91% at 200 µg/mL concentration). The levels of iNOS were reduced up to 67.50%. Moreover, this subextract slightly reduced the phosphorylation levels of MAP kinases (p-ERK, p-JNK, p-38). The highest inhibition was observed for ERK phosphorylation, which was inhibited by 20.53% at 200 µg/mL concentration. Through activity-guided fractionation procedures, kaempferol, kaempferol 3-O-β-glucopyranoside and chlorogenic acid were isolated as the main active components. The structures of the active compounds were determined by 2D-NMR techniques and HRMS analysis. All compounds significantly inhibited NO productions. Results of the present study supported the traditional use of N. oleander flowers to treat inflammatory complaints.

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Section: Structure Elucidation Of the Active Componentsmentioning

confidence: 93%

Section: Structure Elucidation Of the Active Componentsmentioning

confidence: 97%

Evaluation of the in vitro Anti-inflammatory Activity of Nerium oleander L. Flower Extracts and Activity-Guided Isolation of the Active Constituents

Balkan¹,

Gören²,

Kırmızıbekmez³

et al. 2018

Rec.Nat.Prod.

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“…[22][23][24] RV is a naturally occurring polyphenolic phytoalexin found in many plants and contained in foods and drinks such as in Mediterranean diets and French wine, and it is known as a poor water-soluble antioxidant. 25,26) Regarding concerns about the bioavailability of polyphenols, we examined the effects of short-term treatment with each polyphenol. Furthermore, whether or not short-term treatment with polyphenols can improve endothelial dysfunction, impaired NO production, and increased endothelium-derived contraction factors related to diabetes is unknown.…”

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confidence: 99%

Effect of Short-term Polyphenol Treatment on Endothelial Dysfunction and Thromboxane A<sub>2</sub> Levels in Streptozotocin-Induced Diabetic Mice

Taguchi

Hida

Matsumoto

et al. 2014

Biological & Pharmaceutical Bulletin

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Diabetes is characterized by the development of endothelial dysfunction, which affects both nitric oxide (NO)-mediated relaxation and endothelium-derived contracting factors, associated with vascular oxidative stress. There is a growing body of evidence suggesting that polyphenols have several beneficial effects, such as antioxidant and anti-inflammatory activities. This study investigated whether short-term treatment with polyphenols (chlorogenic acid (CA), morin (MO), resveratrol (RV)) can improve endothelial dysfunction related to diabetes. Aorta reactivity was determined in organ chambers, and we measured NO production and thromboxane B 2 (TXB 2 ; a metabolite of TXA 2 ) from aortas in response to acetylcholine (ACh). Streptozotocin (

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“…Chlorogenic acid and rutin were polyphenols found in the highest concentrations in plant extract and have been implicated in inhibiting inflammatory transcription factors (Palikova et al 2009;Zdarilova et al 2010). In addition, Hwang et al (2014) demonstrated that chlorogenic acid and ferulic acid, two constituents present in the studied extract, attenuated pro-inflammatory cytokines including IL-1 and TNF-a in a dose-dependent manner. Therefore, the anti-inflammatory activity of H. cheirifolia methanol extract might be due to the presence of bioactive constituents such as chlorogenic acid, 4-hydroxy benzoic acid, ferulic acid, p-coumaric acid, cinnamic acid or rutin determined by HPLC-TOF/MS in this study.…”

Section: Discussionmentioning

confidence: 84%

Phytochemical screening and anti-inflammatory properties of AlgerianHertia cheirifoliamethanol extract

Bouriche

Kada

Assaf

et al. 2016

Pharmaceutical Biology

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Context: Hertia cheirifolia L. (Asteraceae) is traditionally used in Northern Africa to treat various inflammatory infections. However, few studies on this plant have been reported. Objective: The anti-inflammatory activity of methanol extract of H. cheirifolia leaves was investigated using different experimental models. Materials and methods: Phytochemical analysis was performed to determine phenolic compounds. Acute toxicity of the extract (2000 mg/kg) was examined in Swiss albino mice for 14 days, before croton oilinduced ear oedema in mice, carrageenan-induced paw oedema in Swiss albino rats, cotton pellet-induced granuloma in rats and carrageenan-induced air pouch in mice were conducted. The IL-1b and TNF-a release from concanavalin A-stimulated monocytes was measured by ELISA. Results: Methanol extract of H. cheirifolia is rich in polyphenols and flavonoids. Cinnamic acid and rutin represent the major constituents. Methanol extract up to 2000 mg/kg did not produce any toxic effects. Topical application of 2 mg/ear of the extract produced 78.7% of inhibition on ear swilling. Oral pre-treatment of rats with 200 and 400 mg/kg of the extract inhibited paw oedema by 70% and 89%, respectively. At 200 mg/kg, granuloma dry and wet weights were reduced by 41.85% and 61.72%, respectively. Moreover, the treatment with methanol extract at 1 mg/kg exerted 62.7% of inhibition on leucocytes migrated into the ear pouch. TNF-a and IL-1b release was reduced by 69% and 78%, respectively, with 1 lg/mL of the extract. Conclusion: Methanol extract of H. cheirifolia possesses a strong anti-inflammatory activity and may be considered an interesting source of effective anti-inflammatory compounds.ARTICLE HISTORY

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Anti-inflammatory effects of chlorogenic acid in lipopolysaccharide-stimulated RAW 264.7 cells

Abstract: Chlorogenic acid may be beneficial for the prevention and treatment of anti-inflammatory diseases.

Cited by 361 publications

References 32 publications

Evaluation of the in vitro Anti-inflammatory Activity of Nerium oleander L. Flower Extracts and Activity-Guided Isolation of the Active Constituents

Evaluation of the in vitro Anti-inflammatory Activity of Nerium oleander L. Flower Extracts and Activity-Guided Isolation of the Active Constituents

Effect of Short-term Polyphenol Treatment on Endothelial Dysfunction and Thromboxane A<sub>2</sub> Levels in Streptozotocin-Induced Diabetic Mice

Phytochemical screening and anti-inflammatory properties of AlgerianHertia cheirifoliamethanol extract

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