Evaluation of the in vitro Anti-inflammatory Activity of Nerium oleander L. Flower Extracts and Activity-Guided Isolation of the Active Constituents

Balkan, İrem Atay; Gören, Ahmet; Kırmızıbekmez, Hasan; Yeşilada, Erdem

doi:10.25135/rnp.15.17.05.100

Cited by 19 publications

(9 citation statements)

References 21 publications

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“…As mentioned in earlier studies that IL-1β and IL-6 induced during chronic inflammation [30,31,32]. In the present in silico study flavonoids such as Kaempferol and Chlorogenic acid of N. oleander are suitable in relation to binding energy and molecular interaction with amino acids of target receptors when compared to Indomethacin, which is an evidence of experimental study that flavonoids are suitable for anti-inflammation [4]. In other study, a flavonoid…”

Section: Resultssupporting

confidence: 64%

“…The flavonoids viz. kaempferol and chlorogenic acid from Nerium oleander and Indomethacin as synthetic ligand were selected as per literature [4]. For all the selected compounds, canonical [23], to determine some specific contacts between the atoms of the test compounds and amino acids of the IL-β and IL-6.…”

Section: Selection Of Ligandsmentioning

confidence: 99%

“…Among several phytocompounds in crude extracts of this medicinal plant are well-known for experimental study. In experimental study, the flavonoids such as kaempferol, kaempferol 3-O-β-glucopyranoside and chlorogenic acid from Nerium oleander are suitable phytochemicals for the prevention of pain and inflammation [4]. There are several inflammatory mediators to make reaction during inflammation.…”

Section: Introductionmentioning

confidence: 99%

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2019

RJLBPCS

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The objective was to predict the binding affinity and energy of established phytochemicals as flavonoids (Kaempferol and Chlorogenic acid) of Nerium oleander compared to synthetic drug (Indomethacin) against two proinflammatory cytokine receptors especially interleukins through molecular docking and interaction along with druggability assessment of these small molecules. The software, PyRx (Version 0.8) for the structure-based virtual screening to know receptor-ligand binding affinity and energy. These interleukins as receptors were obtained (PDB IDs: 2NVH and 1P9M) from the European Protein Data Bank (PDBe) and the information on selected flavonoids (phytochemicals) and one synthetic ligand (Indomethacin) were obtained from PubChem database. The prediction of pharmacokinetics, bioavailability and druglikeness for these small molecules was done by using SwissADME online tool. Present computational prediction (molecular docking) indicates that favourable binding energy (Kcal/mol) was observed in Chlorogenic acid (-7.0) followed by Kaempferol (-6.8) while Kaempferol (-7.7) followed by Chlorogenic acid (-7.1) of N. oleander when compared to Indomethacin (-6.7) on IL-1β and IL-6 receptors.The pharmacokinetics, bioavailability and druglikeness predictions showed Kaempferol can be suitable drug candidate. Present in silico study by using software, the phytoligands Chlorogenic acid and Kaempferol of N. oleander may be considered as lead molecules to inhibit the activity of these interleukins while drug candidate may only be considered as Kaempferol, which may prevent inflammation and pain. In future, further functional (in vivo and in vitro) assay is suggested to validate the present predictive results.

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Section: Resultssupporting

confidence: 64%

Section: Selection Of Ligandsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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2019

RJLBPCS

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“…This pathology, which can also occur as a result of the immune system attacking the synovial membrane, is accompanied by swelling, stiffness, pain, and a reduction or loss of joint function [4]. During the establishment and development of rheumatoid arthritis, many inflammatory mediators play a key role in bone destruction and inflammation of the synovial membrane, including tumor necrosis factor (TNF- α ), interleukin-1 β , interleukin-6, nitric oxide (NO), prostaglandins, reactive oxygen species (ROS), platelet-activating factor, leukotrienes, enzymes (lipoxygenases, cyclooxygenases (COX-1 and COX-2), and phospholipases) [5, 6].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Anti-Inflammatory and In Vivo Antiarthritic Activities of Aqueous and Ethanolic Extracts of Dissotis thollonii Cogn. (Melastomataceae) in Rats

Nguemnang

Tsafack

Mbiantcha

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Dissotis thollonii Cogn. (Melastomataceae) is a tropical plant widely used in traditional Cameroonian medicine to relieve and treat many pathologies. It is widespread in the western region where it is used to treat typhoid fever, gastrointestinal disorders, and inflammatory diseases. The purpose of this study is to scientifically demonstrate the anti-inflammatory and antiarthritic properties of the aqueous and ethanolic extracts of the leaves of Dissotis thollonii. The anti-inflammatory properties were evaluated in vitro by inhibition tests for cyclooxygenase, 5-lipoxygenase, protein denaturation, extracellular ROS production, and cell proliferation; while antiarthritic properties were evaluated in vivo in rats using the zymosan A-induced monoarthritis test and the CFA-induced polyarthritis model. This study shows that aqueous and ethanolic extracts at a concentration of 1000 μg/ml inhibit the activity of cyclooxygenase (47.07% and 63.36%) and 5-lipoxygenase (66.79% and 77.7%) and protein denaturation (42.51% and 44.44%). Similarly, both extracts inhibited extracellular ROS production (IC50 = 5.74 μg/ml and 2.96 μg/ml for polymorphonuclear leukocytes, 7.47 μg/ml and 3.28 μg ml for peritoneal macrophages of mouse) and cell proliferation (IC50 = 16.89 μg/ml and 3.29 μg/ml). At a dose of 500 mg/kg, aqueous and ethanolic extracts significantly reduce edema induced by zymosan A (69.30% and 81.80%) and CFA (71.85% and 79.03%). At the same dose, both extracts decreased sensitivity to mechanical hyperalgesia with 69.00% and 70.35% inhibition, respectively. Systemic and histological analyzes show that both extracts maintain the studied parameters very close to normal and greatly restored the normal architecture of the joint in animals. Dissotis thollonii would therefore be a very promising source for the treatment of inflammatory diseases.

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“…For e.g. seed drug shows antioxidant activity (Hussain et al, 2016), leaf drug showsantidiabetic activity (Arumugam et al, 2008), bark drug shows-antibacterial activity (Muthkar et al, 2006) flower drug shows -anti-inflammatory activity (Balkan et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Quality Control Parameters of Leaf and Stem of Phyla nodiflora L. Greene (Verbenaceae)

Trivedi¹,

Donga²,

Pande³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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Phyla nodiflora L. Greene (Verbenaceae) is a medicinal plant commonly known as Ratoliya in Gujarati and Bhujokra in Hindi. Traditionally it is used to cure many diseases like diarrhoea, ulcers, asthma, bronchitis, knee joint pain, gonorrhoea and also there are many reported pharmacological activities like antibacterial, antifungal, antiinflammation, antiulcer, antidiuretic, etc. Thus, it is very essential to lay down quality control parameters so that the therapeutic efficacy of the plant can be maintained. In order to achieve this, in the present work, Phyla nodiflora leaf and stem were evaluated for their physicochemical, phytochemical and pharmacognostic parameters. For phytochemical analysis, different phytoconstituents like saponins, tannins, glycosides, phenols, triterpenes, flavonoids, steroids, etc. were evaluated. For physicochemical analysis, different ash values, extractive values and loss on drying was determined following all standard procedures. Both leaf and stem were rich in phenols, flavonoids and alkaloids; however, leaf had steroids and stem had saponins in maximum amount. The ash values were more in leaf than in stem while loss on drying was almost same (> 10%) in both the parts. Likewise, extractive value was maximum in methanol and minimum in petroleum ether. The diagnostic characters laid down in this study will be helpful for its correct identification and ensure its purity. They are the most simple, easy and cheapest quality control parameters.

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Evaluation of the in vitro Anti-inflammatory Activity of Nerium oleander L. Flower Extracts and Activity-Guided Isolation of the Active Constituents

Cited by 19 publications

References 21 publications

Untitled

Untitled

In Vitro Anti-Inflammatory and In Vivo Antiarthritic Activities of Aqueous and Ethanolic Extracts of Dissotis thollonii Cogn. (Melastomataceae) in Rats

Comparison of Quality Control Parameters of Leaf and Stem of Phyla nodiflora L. Greene (Verbenaceae)

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