Anti-inflammatory Effect of Allium hookeri Root Methanol Extract in LPS-induced RAW264.7 Cells

김창현,; 이미애,; 김태운,; 장자영,; 김현주,

doi:10.3746/jkfn.2012.41.11.1645

Cited by 53 publications

(22 citation statements)

References 16 publications

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“…In this report, we showed that A. hookeri extracts exhibited strong antitumour activity, which may be related to NO production by macrophages. Previous study by Kim et al (2012) reported the anti-inflammatory effect of A. hookeri in LPS-induced mouse macrophage cells. In that test, the treatment with methanol extracts of roots significantly inhibited LPS-induced nitric oxide formation in dose-dependent manner and also decreased TNF-a and IL-6 production.…”

Section: Discussionmentioning

confidence: 99%

“…Supplementation of standard diet with fermented and nonfermented A. hookeri powder has various effects, including regulation of blood lipid levels and antidiabetic activities, in type 2 diabetic mice Lee et al, 2015b). Additionally, an in vitro study characterised the anti-inflammatory and antioxidant effects of A. hookeri in rat macrophages (Kim et al, 2012). However, until now, no reports exist describing the effects of A. hookeri in chickens.…”

Section: Discussionmentioning

confidence: 99%

“…The root of A. hookeri contains an abundance of organo-sulphur compounds, volatile sulphur compounds, proteins, prostaglandins, fructans, vitamins, and polyphenols as well as Allicin, the major flavour compound of garlic (A. sativum) (Dziri et al, 2012). Allicin and organic sulphur compounds are known to reduce cholesterol levels, decrease the risk of heart attack, and exert anti-inflammatory effects (Bae and Bae, 2012;Kim et al, 2012). However, few studies have characterised the effects of these medicinal plants on immunity.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Analysis of the Immunomodulating Effects of Allium Hookeri on Lymphocytes, Macrophages, and Tumour Cells

Lee

Jeong

et al. 2017

Jpn. Poult. Sci.

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The present study investigated the effects of ethanol extracts of Allium hookeri (leaf, root, and fermented root) on parameters of innate immunity, tumour cell viability and antioxidant effect in vitro. Innate immunity was measured by spleen lymphocyte proliferation, nitric oxide production by chicken macrophage HD11 cells and suppressive effect on tumour cell viability was assessed using chicken RP9 cells. Free radical scavenging capacity as a measure of antioxidant capacity was determined by 0.15 mM of DPPH solution. In vitro culture of chicken spleen lymphocytes with ethanol extract of Allium hookeri (62.5-500 μg/mL) significantly induced higher proliferation compared with media control. Stimulation of macrophages with ethanol extract of Allium hookeri (62.5-500 μg/mL) showed increased Nitric oxide production. Tumor cells growth was significantly inhibited by extracts of Allium hookeri at 15.6-125 μg/mL compared with medium control and all extracts exhibited greater than 80% scavenging activity at 1000 μg/mL compared with ethanol vehicle control. Above all, fermented root extracts showed strongest effects on antioxidant activity compared to leaf and root extracts.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In Vitro Analysis of the Immunomodulating Effects of Allium Hookeri on Lymphocytes, Macrophages, and Tumour Cells

Lee

Jeong

et al. 2017

Jpn. Poult. Sci.

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“…hookeri has recently gained significant attention because it produces many useful compounds, such as high amounts of phenolic antioxidants, phytosterols, fiber, ascorbic acid, flavonoids, and allicin, as well as unknown organic sulfur compounds distinct from those of A. cepa (Ayam, 2011;Bae and Bae, 2012;Kala, 2005;Kim et al, 2012;Pandey et al, 2008;Rhyu and Won et al, 2013). Allicins are sulfurcontaining compounds which have been demonstrated to have potent pharmacological properties, including antimicrobial activity, the ability to lower cholesterol levels, antiplatelet aggregation effects, the ability to lower the risk of heart attacks, anti-carcinogenic activity, and antiinflammatory effects (Ayam, 2011;Bae and Bae, 2012;Kim et al, 2012;Rahman and Lowe, 2006;Rhyu and Park, 2013). However, the chemical structure of many novel compounds in A. hookeri and their biological effects on health remain to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

High-frequency Plant Regeneration from Cultured Flower Bud Receptacles of Allium hookeri L.

Koo¹

2014

HST

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Allium hookeri L. (Alliaceae family) is an important ethnomedicinal plant native to the Himalayan region of Asia. The aim of this research was to establish a high-frequency plant regeneration system for in vitro propagation of A. hookeri. Among the tissue types examined, receptacle explants derived from immature flower buds showed the highest regeneration rate of shoots (93.33 ± 4.63%), roots (76.67 ± 7.85%), and calli (80.00 ± 7.43%) when cultured on Gamborg B5 (B5) medium containing 10 μM 6-benzylaminopurine (BA) + 1 μM naphthalene acetic acid (NAA), 0.5 μM BA + 5 μM NAA, and 1 μM BA + 10 μM NAA, respectively. Shoot multiplication was superior when cultured in liquid rather than on solid medium and relatively high concentrations of BA, ranging from 5 to 10 μM. Efficient bulblet formation following root induction from shoot clumps was achieved with culture in liquid B5 medium containing 7% (w/v) sucrose. Regenerated bulblets were successfully acclimatized to ex vitro conditions with a greater than 95% survival rate. By this method, a maximum of 62 plantlets per receptacle could be propagated within 9 weeks of initial culture. The in vitro propagation system established in this study will promote A. hookeri biotechnology, including large-scale production of healthy and aseptic clones, preserving parental genotypes with desirable traits, and genetic manipulation to enhance medicinal value.

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“…Allium hookeri (A. hookeri), a member of Liliaceae family, is widely found in Sri Lanka, India, China and Bhutan, and has anti-oxidant and anti-inflammatory activities [3,7,12]. Water extract of A. hookeri protected oxidative stress-mediated inflammatory responses and pancreatic β-cell damage in streptozotocin-induced diabetic rats [12].…”

Section: Introductionmentioning

confidence: 99%

Allium Hookeri Extract Enhances Glucose Uptake through GLUT4 Up-regulation in 3T3-L1 Cells

Kang¹,

Choi²,

Park³

et al. 2017

Journal of Life Science

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Diabetes mellitus is associated with insulin resistance, which leads to down-regulation of insulin signaling and the decreased glucose uptake. Adipocytes are sensitive to insulin, and closely implicated in insulin resistance and diabetes. Insulin stimulates differentiation of preadipocytes to adipocytes, and increases glucose transport. Allium species have been used as traditional medicine and health-promoting foods. Allium hookeri (A. hookeri) is reported to improve the pancreatic β-cell damage and exhibit pancreatic anti-inflammatory activity in streptozotocin-induced diabetic rats. We investigated whether A. hookeri extract (AHE) may stimulate glucose uptake in adipocytes through increasing insulin sensitivity. AHE enhanced fat accumulation, a differentiation biomarker, under the partial induction of differentiation by insulin. PPARγ, a transcription factor highly expressed in adipocytes, promotes adipocyte differentiation and insulin sensitivity. AHE increased the differentiation of preadipocytes through up-regulation of PPARγ. The activation of PPARγ increases the GLUT4 expression during adipocyte differentiation. GLUT4 is responsible for glucose uptake into the adipocytes. AHE increased the expression of GLUT4 in adipocytes, and subsequently enhanced the insulin-stimulated glucose uptake. These results suggest that AHE promotes adipocyte differentiation through activation of PPARγ, and leads to enhance glucose uptake in adipocytes along with GLUT4 up-regulation. Thus, AHE may be effective for the insulin-sensitizing and anti-diabetic activities.

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Anti-inflammatory Effect of Allium hookeri Root Methanol Extract in LPS-induced RAW264.7 Cells

Cited by 53 publications

References 16 publications

<i>In Vitro </i>Analysis of the Immunomodulating Effects of <i>Allium Hookeri </i>on Lymphocytes, Macrophages, and Tumour Cells

<i>In Vitro </i>Analysis of the Immunomodulating Effects of <i>Allium Hookeri </i>on Lymphocytes, Macrophages, and Tumour Cells

High-frequency Plant Regeneration from Cultured Flower Bud Receptacles of Allium hookeri L.

Allium Hookeri Extract Enhances Glucose Uptake through GLUT4 Up-regulation in 3T3-L1 Cells

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