&lt;i&gt;In Vitro &lt;/i&gt;Analysis of the Immunomodulating Effects of &lt;i&gt;Allium Hookeri &lt;/i&gt;on Lymphocytes, Macrophages, and Tumour Cells

Lee, Youngsub; Lee, Sung Hyen; Jeong, Mi Sun; Kim, Jung Bong; Choe, Jeong‐Sook; Kim, Dong Wook; Lillehoj, Hyun S.

doi:10.2141/jpsa.0160108

Cited by 14 publications

(16 citation statements)

References 28 publications

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“…Total phenolic content (TPC) analysis was modified from the method of Senhaji et al [ 13 ]. Twenty microliters of samples (50, 100, 250, 500, and 1000 μg/mL) were mixed with 80 μL of distilled water and 40 μL of 100% Folin–Ciocalteu’s phenol reagent (Sigma-Aldrich Co., St. Louis, MO, USA) in a 96-well plate, and it was reacted for 3 min.…”

Section: Methodsmentioning

confidence: 99%

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Antioxidant and Immune Stimulating Effects of Allium hookeri Extracts in the RAW 264.7 Cells and Immune-Depressed C57BL/6 Mice

et al. 2022

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We investigated the antioxidant and immune-enhancing effects of the extracts from Allium hookeri leaves and roots (AHL and AHR) in in vitro and in vivo models. Their antioxidant effects were determined by total phenolic content (TPC), DPPH and ABTS radical scavenging activities, and superoxide dismutase and catalase activities. The immunomodulatory effects were evaluated by nitric oxide (NO) production and cytokine concentrations produced from RAW 264.7, and by serum IgA and IgG levels, cytokine levels, and NK cell activities in the immunosuppressed C57BL/6 mice. AHL and AHR extracts improved antioxidant activities and productions of NO and cytokines without cytotoxicity in the RAW 264.7 cells. AHL and AHR groups showed significantly higher serum IgA and IgG levels, Th1 cytokine concentrations, splenocyte proliferations, and NK cell activities than the NC group which was not treated with AHL or AHR extract. AHR extract showed higher values than AHL extract in the factors evaluated in this study. The results show that they have high antioxidant and immunomodulatory effects and can be used as novel potential therapeutic candidates to treat related diseases and to improve public health.

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Section: Methodsmentioning

confidence: 99%

“…Organosulfur compounds are considered major chemical markers in AH, showing anti-inflammatory and anti-obesity in previous studies [ 9 , 10 , 11 ]. However, there is only limited information available on the anti-oxidant and immune stimulatory effects of AH leaves [ 13 , 14 ] compared with those of AH roots.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Immune Stimulating Effects of Allium hookeri Extracts in the RAW 264.7 Cells and Immune-Depressed C57BL/6 Mice

et al. 2022

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“…As these components exhibit antioxidant activities, the use of A. hookeri is promising as a medical food [14,15]. A. hookeri exhibits immunomodulatory effects in lymphocytes, macrophages, and tumor cells in in-vitro chicken cell experiments [16]. In vivo experiments have also suggested that A. hookeri inhibits the inflammatory response in the pancreas of diabetic rats and LPS-induced young broiler chickens [17,18].…”

Section: Introductionmentioning

confidence: 99%

Effects of Allium hookeri on gut microbiome related to growth performance in young broiler chickens

et al. 2020

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Healthy food promotes beneficial bacteria in the gut microbiome. A few prebiotics act as food supplements to increase fermentation by beneficial bacteria, which enhance the host immune system and health. Allium hookeri is a healthy food with antioxidant and anti-inflammatory activities. A. hookeri is used as a feed supplement for broiler chickens to improve growth performance. Although the underlying mechanism is unknown, A. hookeri may alter the gut microbiome. In the current study, 16S rRNA sequencing has been carried out using samples obtained from the cecum of broiler chickens exposed to diets comprising different tissue types (leaf and root) and varying amounts (0.3% and 0.5%) of A. hookeri to investigate their impact on gut microbiome. The microbiome composition in the groups supplemented with A. hookeri leaf varied from that of the control group. Especially, exposure to 0.5% amounts of leaf resulted in differences in the abundance of genera compared with diets comprising 0.3% leaf. Exposure to a diet containing 0.5% A. hookeri leaf decreased the abundance of the following bacteria: Eubacterium nodatum, Marvinbryantia, Oscillospira, and Gelria. The modulation of gut microbiome by leaf supplement correlated with growth traits including body weight, bone strength, and infectious bursal disease antibody. The results demonstrate that A. hookeri may improve the health benefits of broiler chickens by altering the gut microbiome.

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“…The spleen lymphocyte transformation capacity can reflect the state of the body's cellular immune function and malignant tumors lead to a decrease in lymphocyte transformation capacity (Lee et al., 2017). The spleen lymphocyte transformation capacity of all test groups was significantly enhanced in a dose‐dependent manner compared with the capacity of the TC group (Table 3).…”

Section: Resultsmentioning

confidence: 99%

Investigation on the biological activity of anthocyanins and polyphenols in blueberry

Yang

Wang

et al. 2021

Journal of Food Science

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Blueberry (Vaccinium spp.) is a fruit recognized in the world as healthy, and many of its active ingredients have important physiological functions. This study analyzed the antioxidant activity, antitumor activity, and immune function of anthocyanins and polyphenols extracted from blueberries. The crude extracts of anthocyanins and polyphenols were obtained from blueberries and then purified, and the extract exhibited excellent dose‐dependent antitumor activity and antioxidant activity in vivo and in vitro. The purified anthocyanins and polyphenol compounds showed higher antioxidant activity, whereas the crude extract had a better inhibitory effect on tumor proliferation than pure extract, and the blueberry anthocyanin and polyphenol crude product mixture showed a more powerful tumor suppressor, which may be the result of the synergistic effect of multiple compounds. The crude extracts were also more efficient at improving immune function, as reflected by measurements of change in body weight, thymus and spleen indices, macrophage phagocytosis, lymphocyte transformation capacity, superoxide dismutase activity, malondialdehyde content, and serum nitric oxide levels. These results indicate that blueberry anthocyanins and polyphenol extracts can improve immune function and reduce the metastasis and proliferation of cancer cells. This study reveals the functions of important active substances in blueberries and provides support for the development of functional health products and therapeutic drugs. Practical Application We compared the biological activity of crude and purified anthocyanins and polyphenol extracts from blueberries and tested their effects on improving immune function. This study contributes to a better understanding of the bioactivity of blueberry extracts and is valuable for further applications of blueberries in medicine.

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<i>In Vitro </i>Analysis of the Immunomodulating Effects of <i>Allium Hookeri </i>on Lymphocytes, Macrophages, and Tumour Cells

Cited by 14 publications

References 28 publications

Antioxidant and Immune Stimulating Effects of Allium hookeri Extracts in the RAW 264.7 Cells and Immune-Depressed C57BL/6 Mice

Antioxidant and Immune Stimulating Effects of Allium hookeri Extracts in the RAW 264.7 Cells and Immune-Depressed C57BL/6 Mice

Effects of Allium hookeri on gut microbiome related to growth performance in young broiler chickens

Investigation on the biological activity of anthocyanins and polyphenols in blueberry

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