Anti-inflammatory and anti-oxidant effect of Calea urticifolia lyophilized aqueous extract on lipopolysaccharide-stimulated RAW 264.7 macrophages

Torres-Rodríguez, María Lucina; García-Chávez, Erika; Berhow, Mark A.; Mejía, Elvira González de

doi:10.1016/j.jep.2016.04.057

Cited by 40 publications

(20 citation statements)

References 31 publications

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“…The nitric oxide assay was modified from the method previously reported by Torres-Rodríguez et al [13]. RAW 264.7 macrophage cells were grown and incubated at 37 • C in a humidified incubator with a 5% carbon dioxide (CO 2 ) atmosphere for 48 h. After, 2 × 10 5 cells were seeded in a six-well plate and incubated at 37 • C in a 5% CO 2 atmosphere for 48 h, the cells were then stimulated with lipopolysaccharides (LPS) and treated with different concentrations of BSS (a mixture of WF1 and WF2) between 0-1000 µg/mL.…”

Section: Nitric Oxide Assaymentioning

confidence: 99%

The Bioactivity Study of Active Compounds in Wolffia globosa Extract for an Alternative Source of Bioactive Substances

2017

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Wolffia globosa is a small plant found in the lagoons in tropical zones. The aim of our study was to examine the biological compounds found in W. globosa and their activities. The substances in W. globosa were extracted, isolated, and their chemical structures ascertained by Fourier transform infrared (FT-IR) spectroscopy and proton nuclear magnetic resonance ( 1 H-NMR) spectroscopy. The extract was tested for bioactivity, including antioxidant, anti-inflammatory, and cytotoxic activities. The results showed that the isolated compounds in fraction two were mainly β-sitosterol and stigmasterol. The sterols found in the extract were able to inhibit nitric oxide production in RAW 264.7 macrophage cells, which implied an anti-inflammatory activity. The extract was found to be non-toxic to human dermal fibroblast cells with an IC 50 of 106.38 ± 37.0 µg/mL.

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Section: Nitric Oxide Assaymentioning

confidence: 99%

The Bioactivity Study of Active Compounds in Wolffia globosa Extract for an Alternative Source of Bioactive Substances

2017

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“…The main active compounds from leaves are germacranolide, sesquiterpene lactones with marked cytotoxicity in vitro against human tumor cell lines HL60 and SW480 [ 42 ]. C. urticifolia extract prepared in boiling water (10 g/100 mL) and freeze-dried (yield 14.8%) was able to modulate anti-inflammatory activity through the suppression of the NF-κB signaling pathway by suppressing the nuclear translocation of p50 and p65 subunits of NF-κB that resulted in reduced iNOS expression, inhibited NO/ROS production and the decreased production of pro-inflammatory markers of chronic low-grade inflammation [ 43 ]. Phenolic compounds such as caffeoylquinic acids and flavonoids glycosides could be responsible for the anti-inflammatory and antioxidant properties of C. urticifolia tea since germacrolides did not inactivate NF-κB as expected and shown in parthenolide [ 42 ].…”

Section: Resultsmentioning

confidence: 99%

Solving the puzzle: what is behind our forefathers anti-inflammatory remedies?

Villanueva

Alonso‐Esteban

Villanueva

2017

J Intercult Ethnopharmacol

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Inflammation is a ubiquitous host response in charge of restoring normal tissue structure and function but is a double-edged sword, as the uncontrolled or excessive process can lead to the injury of host cells, chronic inflammation, chronic diseases, and also neoplastic transformation. Throughout history, a wide range of species has been claimed to have anti-inflammatory effects worldwide. Among them, Angelica sinensis, Tropaeolum majus, Castilleja tenuiflora, Biophytum umbraculum, to name just a few, have attracted the scientific and general public attention in the last years. Efforts have been made to assess their relevance through a scientific method. However, inflammation is a complex interdependent process, and phytomedicines are complex mixtures of compounds with multiple mechanisms of biological actions, which restricts systematic explanation. For this purpose, the omics techniques could prove extremely useful. They provide tools for interpreting and integrating results from both the classical medical tradition and modern science. As a result, the concept of network pharmacology applied to phytomedicines emerged. All of this is a step toward personalized therapy.

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“…In this present result, PE has better anti‐inflammatory effects than γ‐oryzanol because of containing other bioactivities components such as flavonoids, anthocyanidin, and GABA. Flavonoids reduce ROS‐ and free radical‐induced inflammation by the removal of reactive oxygen species (ROS) and free radicals (Belemets et al, ; Torres‐Rodriguez, Garcia‐Chavez, Berhow, & Mejia, ). Other than that, flavonoids and anthocyanin ameliorate hyperglycemia and hyperlipidemia by improving insulin sensitivity, glucose, and lipid homeostasis (Beseni et al, ; Dada et al, ).…”

Section: Discussionmentioning

confidence: 99%

Efficiency comparison of PGBR extract and γ‐oryzanol in antioxidative stress and anti‐inflammatory properties against metabolic syndrome

Lin

Shen

et al. 2019

J Food Biochem

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This research aims to delineate the anti‐inflammatory effect of pregerminated brown rice extract (PE) and γ‐oryzanol on improving metabolic features of high‐fat diet (HFD)‐induced metabolic syndrome (MetS) mouse model. C57BL/6 mice were randomly divided into eight groups: regular diet (RD), HFD, HFD‐combined treatment of 0.5, 5, or 10 mg kg−1 day−1 oral gavage γ‐oryzanol, and 30, 300, or 600 mg kg−1 day−1 PE for 18 weeks. HFD‐fed mice showed overweight, hyperglycemia, hyperlipidemia signs of metabolic disorder, and elevation of inflammatory cytokines such as IL‐6, TNF‐α, IFN‐γ, NO, PGE2 in serum and MAPKs, transcription factor p65, iNOS, and MDA in the liver. In contrast, HFD‐fed mice showed lower levels of adiponectin in serum and antiperoxidation enzymes GPx, SOD, and catalase in the liver. While HFD‐fed mice cotreated with PE or γ‐oryzanol, HFD‐induced metabolic disorders, ROS, and inflammation were improved. The anti‐MetS, antioxidative stress and anti‐inflammation properties of PE were more potent than γ‐oryzanol. Practical applications Our study showed that PE or γ‐oryzanol supplement could help control metabolic disorders, oxidative stress, chronic inflammation, and related complications.

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Anti-inflammatory and anti-oxidant effect of Calea urticifolia lyophilized aqueous extract on lipopolysaccharide-stimulated RAW 264.7 macrophages

Cited by 40 publications

References 31 publications

The Bioactivity Study of Active Compounds in Wolffia globosa Extract for an Alternative Source of Bioactive Substances

The Bioactivity Study of Active Compounds in Wolffia globosa Extract for an Alternative Source of Bioactive Substances

Solving the puzzle: what is behind our forefathers anti-inflammatory remedies?

Efficiency comparison of PGBR extract and γ‐oryzanol in antioxidative stress and anti‐inflammatory properties against metabolic syndrome

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Anti-inflammatory and anti-oxidant effect of Calea urticifolia lyophilized aqueous extract on lipopolysaccharide-stimulated RAW 264.7 macrophages

Cited by 40 publications

References 31 publications

The Bioactivity Study of Active Compounds in Wolffia globosa Extract for an Alternative Source of Bioactive Substances

The Bioactivity Study of Active Compounds in Wolffia globosa Extract for an Alternative Source of Bioactive Substances

Solving the puzzle: what is behind our forefathers&#146; anti-inflammatory remedies?

Efficiency comparison of PGBR extract and γ‐oryzanol in antioxidative stress and anti‐inflammatory properties against metabolic syndrome

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Solving the puzzle: what is behind our forefathers anti-inflammatory remedies?