Anti-inflammatory Activity Test of Astaxanthin Extract from Litopenaeus vannamei Shrimp Waste Against the Number of Neutrophils and Lymphocytes in White Rats (Rattus norvegicus) Injected with Carrageenin

Abstract

Shrimp is one of the main export commodities in Indonesia which has the potential to have bioactive compounds. Shrimp waste contains carotenoids, one of which is astaxanthin, a red-orange xanthophyll carotenoid which has potential pharmacological effects including anti-inflammatory. The purpose of this study was to test the anti-inflammatory activity of astaxanthin extract from Litopenaeus vannamei shrimp waste against the decrease in the number of neutrophils and lymphocytes in carrageenin-injected white rats. Tests carried out in this study include extraction, qualitative tests using Thin Layer Chromatography (TLC), Salkowski test, quantitative tests using UV-Vis spectrophotometry, High Performance Liquid Chromatography (HPLC), purification using column chromatography, and preclinical tests using edema models of white rat feet with 1% carrageenin injection and various doses of the fraction given were 50 mg / kgBW, 100 mg / kgBW and 150 mg / kgBW, positive control (celexocib 18 mg / kgBW) and a negative control (propylene glycol 1 ml). The relative values of neutrophils and lymphocytes per 100 cells were statistically analyzed using SPSS, with a repeated measure ANOVA for the parametric test and Friedman test for the non-parametric test. The results showed that Litopenaeus vannamei shrimp waste extract contained terpenoids and astaxanthin with concentrations of 0.52 mg/gr crude extract and 220.77 mg/g fraction. The results of parametric and non-parametric analysis (p> 0.05) showed that there was no significant difference between each treatment in the decrease of the relative value of neutrophils and lymphocytes, at 0, 4 and 8 hour. The relative value of neutrophils and lymphocytes was still within the normal limit, which might also indicate that fraction did not change the relative value of neutrophils and lymphocytes which can cause neutrophilia (increased neutrophils) or neutropenia (decreased neutrophils) and lymphocytopenia (decreased lymphocytes) or ..........

The Effect of Shrimp Shell (Litopenaeus vannamei) Extract on Testicular Parameters of Streptozotocin-induced Diabetic Rats

“…Then, shrimp shells were ground into powder. Dried shrimp shell powder was subjected to a 3-day extraction using a maceration method as described by Najoan et al (2021). Ethanol (70%) was used as the solvent at a 1:10 (v/v) ratio with water.…”

“…The maceration was repeated two times for 3 days, respectively. Subsequently, evaporation was carried out using a rotary evaporator at 5 rpm and 40°C, and then continued in an oven at 40°C until the consistency was like a paste (Najoan et al, 2021).…”

Dietary Astaxanthin Can Promote the Growth and Motivate Lipid Metabolism by Improving Antioxidant Properties for Swimming Crab, Portunus trituberculatus

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