Anti-Inflammatory Activity of Tinocrisposide by Inhibiting Nitric Oxide Production in Lipopolysaccharides-Stimulated Raw 264.7 Cells

Adnan, Adek Zamrud; Taher, Muhammad; Afriani, Tika; Fauzana, Annisa; Roesma, Dewi Imelda; Putra, Andani Eka

doi:10.22159/ajpcr.2018.v11i4.23739

Cited by 6 publications

(7 citation statements)

References 18 publications

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“…f. & Thomson was by far the most commonly reported species for treatments of diabetes in traditional Thai medicine. Apart from its use in diabetes treatments, it has been used as an anti-inflammatory based on its tinocriposide content [65], as a cardioprotective to treat hypertension [48] as well as an immunomodulatory, cytotoxic, and antimalarial herbal drug [66]. Results from several studies have confirmed that T. crispa is effective for diabetes treatment [67][68][69].…”

Section: Discussionmentioning

confidence: 99%

Thai Ethnomedicinal Plants Used for Diabetes Treatment

Phumthum¹,

Balslev²

2018

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Background: Diabetes is a metabolic disorder and a serious global health problem. Over 400 million people suffer from diabetes and it is estimated that the number will dramatically increase in the future. The cost of diabetes treatment for individual patients is very high. However, traditional knowledge could be used to support conventional diabetes treatments. Here, we identify medicinal plants that have been used as treatments for diabetes based on Thai ethnobotanical knowledge. Methods: We present a literature review of data for the use of ethnomedicinal plants for diabetes treatments used by people in Thailand. The data were obtained from 31 original references including theses, reports, journal articles, and books published from 1992-2015. Results: In total, 187 reports of 123 plants species that had been used traditionally to treat diabetes in Thailand were identified. Tinospora crispa (L.) Hook. f. & Thomson, Morinda citrifolia L. and Phyllanthus amarus Schumach. & Thonn were the three most commonly used species. There were also numerous reports of the use of Leguminosae, Lamiaceae, Phyllanthaceae, Rubiaceae, and Acanthaceae for the treatment of diabetes. Interestingly, the grass family (Poaceae), for which there were otherwise few medicinal use reports, ranked third in our data. Stems, roots, and leaves were most commonly used in diabetes

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Section: Discussionmentioning

confidence: 99%

Thai Ethnomedicinal Plants Used for Diabetes Treatment

Phumthum¹,

Balslev²

2018

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“…It is due to the content of tinocrisposid compounds, flavonoids, such as quercetin, aporphine, berberine, and palmatine as antiinflammatory and antimalarial compounds. [13] It inhibits tubular damage caused by immune responses during malaria infection, either by host immune cells or parasites. According to Rathe et al, (2009), the compounds compound flavonoids inhibit the production of Nitric oxide (NO), Inducible Nitrite oxide (INOS) and NF-kβ activation in macrophage cells stimulated by lipopolysaccharides.…”

Section: Histopathological Changes In Tubulesmentioning

confidence: 99%

“…The result is due to antiparasitic and anti-inflammatory effects. [12,13,15,30,31,32] Changes in NF-kβ expression levels…”

Section: Histopathological Changes In Tubulesmentioning

confidence: 99%

“…They are efficient as antimalarial, antipyretic, antidiabetic, anti-inflammatory, and analgesic. [11,12,13,14] We investigated whether brotowali extract can affect the histopathological features and NF-kβ expression on the renal tubules in mice infected by Plasmodium Berghei treated by artesunate. It was based on information about the role of NF-kβ in inflammatory reactions as pathogenesis of malaria in the kidneys and brotowali extract profile as described above.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Artesunate and Brotowali (Tinospora Crispa) Combination on Histopathological, and Expression of Nuclear Factor Kappa B (NF-kÎ²) in Renal Tubules of Mice Infected With Plasmodium Berghei

Merici

Fitri

Budiarti

2020

CRJIM

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Brotowali (BR) extract (Tinospora crispa) can be used as an antimalarial. Aim: to determine the effect of BR extract in histopathological and expression of NFκB in mice tubules infected by Plasmodium berghei treated by artesunate (AR). Method: we used 42 C57BL / 6J strain mice as experimental animals, which were randomly divided into 7 groups : negative control (NC), positive control (PC), treatment group consist of AR 32 mg/kb (group 1); BR 70 mg/kg (group 2), combination of AR+BR 50 mg/kg (group 3), AR+BR 60 mg/kg (group 4), and AR+BR 70 mg/kg (group 5). Histological examination (hematoxylin-eosin (HE) staining) and expression of NFKB (immunohistochemical staining) in the kidneys were performed on 7th and 14th. Result: compared to PC group, BR with doses of 70 mg until 14th day, improved the degree of tubular necrosis, interstitial fibrosis, tubular degeneration, and inflammatory cell infiltration (p <0.001) but did not reach NC group (p <0.05). The combination of AR+BR until the 14th day with dose of 50, 60, 70 mg all of dose improves significantly in-term of degree of tubular cell necrosis and inflammatory cell infiltration. The degree of interstitial fibrosis on 14th day only improved in group 4 and 5 (p<0.001 and p=0.003). The level of NF-kB expression on day 7 and day 14 was reduced in group 2, group 4, and group 5 compared to PC group. There was positive correlation on 7th and 14th between NF-kβ expression and tubular degeneration, tubular cell necrosis, inflammatory cell infiltration, and interstitial fibrosis. Conclusion: the combination of AR+BR extract can improve histopathological features and reduce NF-kβ expression in mice tubules infected by Plasmodium berghei with an optimal dose was 60 mg/day for 7-14 days or 70 mg for 7 days.

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“…Furthermore, in our previous research, various concentrations of tinocrisposide were tested against the LPSstimulated RAW 264.7 cells, and the results showed the decrease of NO level production in concentration activity-dependent manner, with half-maximal inhibition concentration of 46.92 μM. Thus, it can be developed as anti-inflammatory candidate drug because NO is a reactive nitrogen species which is produced by NO synthase [10]. S. Chanda and A.R.…”

Section: Introductionmentioning

confidence: 97%

In Vitro Anti-Inflammatory Activity Test of Tinocrisposide and Freeze-Dried Aqueous Extract of Tinospora Crispa Stems on Human Red Blood Cell by Increasing Membrane Stability Experiment

Adnan

Armin

Sudji

et al. 2019

Asian J Pharm Clin Res

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Objective: This study was aimed to evaluate the anti-inflammatory effect of isolated tinocrisposide and freeze-dried aqueous extract of Tinospora crispa stems on human red blood cell (HRBC) by increasing membrane stability in vitro models. Methods: Anti-inflammatory effect of tinocrisposide and FDAETCS was evaluated by in vitro HRBC membrane stabilization method. The study was separated into two steps which were a hemolytic and a membrane stabilization experiment. The hemoglobin that was released throughout the damaged erythrocytes membrane was then quantified at the wavelength of (λ) 560 nm. Results: The hemoglobin in the HBRC supernatant that treated with tinocrisposide at concentration of 100, 200, 400, 600, 800, and 1000 μg/ml showed an absorbance at λ 560 nm of 0.060, 0.061, 0.071, 0.072, 0.075, and 0.0793, respectively, and the calculated hemolysis percentage was 0.032, 0.097, 1.203, 1.236, 1.641, and 2.079%, respectively. We found a linear correlation between concentration and hemolytic activity of tinocrisposide, with regression equation, y=0.0023x−0.1312 (r=0.929). Meanwhile, the HBRC supernatant that treated with FDAETCS at concentration of 100, 200, 400, 600, and 800 μg/ml showed an absorbance at λ 560 nm of 0.063, 0.064, 0.066, 0.067, and 0.077, respectively, and revealed the hemolytic percentage of 0.347, 0.473, 0.693, 0.992, and 1.896%, respectively. It also gave a linear correlation between FDAETCS concentration and hemolytic activity percentage, with regression equation, y=0.002x+0.0222 (r=0.895). Moreover, in HRBC membrane stability experiment, tinocrisposide concentration of 100, 200, 400, 800, and 1000 μg/ml gave absorbance at λ 560 nm of 0.818, 0.808, 0.798, 0.789, 0.773, and 0.761, respectively, and calculated HRBC membrane stabilization activity as much as 5.437, 6.533, 7.707, 8.748, 10.597, and 12.100%, respectively. Meanwhile, the positive control ibuprofen 25 μg/ml only exerted the membrane stability of 5.620%. It was found a linear correlation between tinocrisposide concentration and membrane stability percentage, with the regression equation, y=0.0072x+4.8312 (r=0.9932). Treated FDAETCS in the concentration of 100, 200, 400, and 800 μg/ml gave the absorbance at λ 560 nm of 0.802, 794, 0.777, 0.791, and 0.792, with stability membrane percentage of 7.283, 8.208, 10.944, 8.555, and 8.401%, respectively. It can be seen that the FDAETCS concentrations and its hemolytic percentage showed a parabolic relationship, which gave a maximum at a concentration of the extract of 400 mg/ml with membrane stabilizing of 10.944%. Conclusion: It can be concluded that tinocrisposide and FDAETCS have an anti-inflammatory activity by increase the membrane stability of lysosome cell that has equal physiological properties with erythrocytes membrane cell and it has no hemolytic activity.

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Anti-Inflammatory Activity of Tinocrisposide by Inhibiting Nitric Oxide Production in Lipopolysaccharides-Stimulated Raw 264.7 Cells

Cited by 6 publications

References 18 publications

Thai Ethnomedicinal Plants Used for Diabetes Treatment

Thai Ethnomedicinal Plants Used for Diabetes Treatment

The Effect of Artesunate and Brotowali (Tinospora Crispa) Combination on Histopathological, and Expression of Nuclear Factor Kappa B (NF-kÎ²) in Renal Tubules of Mice Infected With Plasmodium Berghei

In Vitro Anti-Inflammatory Activity Test of Tinocrisposide and Freeze-Dried Aqueous Extract of Tinospora Crispa Stems on Human Red Blood Cell by Increasing Membrane Stability Experiment

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