In Vitro Anti-Inflammatory Activity Test of Tinocrisposide and Freeze-Dried Aqueous Extract of Tinospora Crispa Stems on Human Red Blood Cell by Increasing Membrane Stability Experiment

Adnan, Adek Zamrud; Armin, Fithriani; Sudji, Ikhwan Resmala; Novida, Mediola Dwi; Roesma, Dewi Imelda; Ali, Hana Afifah; Fauzana, Annisa

doi:10.22159/ajpcr.2019.v12i5.30690

“…The in vitro anti-in ammatory activity was evaluated using the membrane stabilization technique (Adnan et al 2019;Mir et al 2022). Among all the tested polymeric ammonium salts, polymer VI (QMCC-g-PAN) containing tris amine moiety signi cantly inhibits (p ≤ 0.05) the rupturing of the erythrocyte membranes caused by the hypotonicity with a value of 74% at a dosage of 1000 µg/ml (see Tables 3a-c).…”

Section: Membrane Stabilization Assaymentioning

confidence: 99%

Ammonium salts of microcrystalline cellulose-g-poly(acrylonitrile): toxicity, antioxidant and anti-inflammatory properties

Kenawy

¹

,

Khattab

²

,

Tenhu

³

et al. 2023

Preprint

0

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Cellulose is an excellent starting material for the construction of bioactive polymers. In the present work, we have synthesized quaternized graft copolymers of cellulose and tested their biological properties. Cellulose was grafted with acrylonitrile in a cerium ion catalyzed reaction. High yield of the grafting reaction, 89%, was achieved. Next, the poly (acrylonitrile) chains were aminated using three different amines and finally, the amino functions were quaternized using methyl iodide. In addition to chemical and physical characterization of the polymers, several tests on their bioactive properties have been conducted. The polymers turned out to have good antioxidant properties, as assessed studying how they scavenge ABTS radicals. Anti-inflammatory properties were investigated by a membrane stabilization method. The results showed that the quaternized polymers had anti-inflammatory effects and the one aminated with tris(2-aminoethyl)amine was the most significant compared with indomethacin. The cytotoxicity was evaluated in vitro against HepG2 and WI-38 cell lines. All quaternized polymers showed moderate effects against the cancerous cell line HEPG2. On the other hand, their effect against normal fibroblast WI-38 was weak. The acute toxicity in vivo was evaluated for one of the polymers, for which the LD50 was 6606 mg/kg. The high LD50 indicates the polymer is relatively non-toxic, and will be considered in future for in vivo studies.

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“…The content of flavonoids and terpenoids in the S. littoralis extract is believed to be able to stabilize the lysosomal membrane by binding cations. Tinocrisposide compounds, a terpenoid group from the Brotowali plant (Tinospora crispa) showed an increase the stability of erythrocyte membranes (Adnan et al, 2019).…”

Section: Figure 1 the Incubation Process Of The Erythrocyte Hemolysis...mentioning

confidence: 99%

In Vitro Antiinflammatory Activity of Bajakah (Spatholobus littoralis) Stem Extract

Rousdy,

Wardoyo

2023

J Bio Bio Edu

0

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The plant of Bajakah tampala (Spatholobus littoralis Hassk) has been utilized in traditional medication. Previous studies have proven the existence of in vivo anti-inflammatory activities of Bajakah plant (S. littoralis) in lowering the degree of carrageenan-induced paw oedema in mice. This study aims to determine the anti-inflammatory mechanism of S. littoralis extract in vitro through an approach of enzyme inhibition involved in the inflammatory reaction. The concentration of ethanol extract of Bajakah used was 0.1; 0.2; 0.4; 0.8; 1.6 mg/ml. The parameters measured were lipoxygenase enzyme inhibition, protein denaturation inhibition, protease enzyme inhibition, as well as plasma membrane stabilization. The results of the study showed the potential of the ethanol extract of Bajakah stems in inhibiting the inflammatory process viewed from the ability to inhibit inflammation-related enzymes. S. littoralis extract concentration of 1.6 mg/ml showed the best inhibition of the protein denaturation process (75.9%), the inhibition of trypsin protease enzyme (26.1%) and the stability of erythrocyte membrane (93.7%). However, the extracts of S. littoralis did not provide inhibition for the lipoxygenase enzyme in the range of 0.2-3.8%. This study proves the role of S. littoralis extract in the anti-inflammatory mechanism. It has the potential to be developed into standardized herbs.

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Ammonium salts of microcrystalline cellulose-g-poly (acrylonitrile): toxicity, antioxidant and anti-inflammatory properties

Kenawy,

Khattab,

Tenhu

et al. 2023

Cellulose

1

0

View full text Add to dashboard Cite

Cellulose is an excellent starting material for the construction of bioactive polymers. In the present work, we have synthesized quaternized graft copolymers of cellulose and tested their biological properties. Cellulose was grafted with acrylonitrile in a cerium ion catalyzed reaction. High yield of the grafting reaction, 89%, was achieved. Next, the poly (acrylonitrile) chains were aminated using three different amines and finally, the amino functions were quaternized using methyl iodide. In addition to chemical and physical characterization of the polymers, several tests on their bioactive properties have been conducted. The polymers turned out to have good antioxidant properties, as assessed studying how they scavenge ABTS radicals. Anti-inflammatory properties were investigated by a membrane stabilization method. The results showed that the quaternized polymers had anti-inflammatory effects and the one aminated with tris(2-aminoethyl)amine was the most significant compared with indomethacin. The cytotoxicity was evaluated in vitro against HepG2 and WI-38 cell lines. All quaternized polymers showed moderate effects against the cancerous cell line HEPG2. On the other hand, their effect against normal fibroblast WI-38 was weak. The acute toxicity in vivo was evaluated for one of the polymers, for which the LD50 was 6606 mg/kg. The high LD50 indicates the polymer is relatively non-toxic, and will be considered in future for in vivo studies. Graphical abstract

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In Vitro Anti-Inflammatory Activity Test of Tinocrisposide and Freeze-Dried Aqueous Extract of Tinospora Crispa Stems on Human Red Blood Cell by Increasing Membrane Stability Experiment

Cited by 9 publications

References 18 publications

Ammonium salts of microcrystalline cellulose-g-poly(acrylonitrile): toxicity, antioxidant and anti-inflammatory properties

Ammonium salts of microcrystalline cellulose-g-poly(acrylonitrile): toxicity, antioxidant and anti-inflammatory properties

In Vitro Antiinflammatory Activity of Bajakah (Spatholobus littoralis) Stem Extract

Ammonium salts of microcrystalline cellulose-g-poly (acrylonitrile): toxicity, antioxidant and anti-inflammatory properties

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