Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis

Abstract

BackgroundNanocrystalline silver dressings have anti-inflammatory activity, unlike solutions containing Ag+ only, which may be due to dissolution of multiple silver species. These dressings can only be used to treat surfaces. Thus, silver-containing solutions with nanocrystalline silver properties could be valuable for treating hard-to-dress surfaces and inflammatory conditions of the lungs and bowels. This study tested nanocrystalline silver-derived solutions for anti-inflammatory activity.MethodsInflammation was induced on porcine backs using dinitrochlorobenzene. Negative and positive controls were treated with distilled water. Experimental groups were treated with solutions generated by dissolving nanocrystalline silver in distilled water adjusted to starting pHs of 4 (using CO2), 5.6 (as is), 7, and 9 (using Ca(OH)2). Solution samples were analyzed for total silver. Daily imaging, biopsying, erythema and oedema scoring, and treatments were performed for three days. Biopsies were processed for histology, immunohistochemistry (for IL-4, IL-8, IL-10, TNF-α, EGF, KGF, KGF-2, and apoptotic cells), and zymography (MMP-2 and -9). One-way ANOVAs with Tukey-Kramer post tests were used for statistical analyses.ResultsAnimals treated with pH 7 and 9 solutions showed clear visual improvements. pH 9 solutions resulted in the most significant reductions in erythema and oedema scores. pH 4 and 7 solutions also reduced oedema scores. Histologically, all treatment groups demonstrated enhanced re-epithelialisation, with decreased inflammation. At 24 h, pMMP-2 expression was significantly lowered with pH 5.6 and 9 treatments, as was aMMP-2 expression with pH 9 treatments. In general, treatment with silver-containing solutions resulted in decreased TNF-α and IL-8 expression, with increased IL-4, EGF, KGF, and KGF-2 expression. At 24 h, apoptotic cells were detected mostly in the dermis with pH 4 and 9 treatments, nowhere with pH 5.6, and in both the epidermis and dermis with pH 7. Solution anti-inflammatory activity did not correlate with total silver content, as pH 4 solutions contained significantly more silver than all others.ConclusionsNanocrystalline silver-derived solutions appear to have anti-inflammatory/pro-healing activity, particularly with a starting pH of 9. Solutions generated differently may have varying concentrations of different silver species, only some of which are anti-inflammatory. Nanocrystalline silver-derived solutions show promise for a variety of anti-inflammatory treatment applications.

Silver Nanoparticles as Real Topical Bullets for Wound Healing

“…In vitro and in vivo experimental findings show that silver nanoparticles are effective at decreasing inflammation in peritoneal adhesions without significant toxic effects. 99 Nadworny et al 100 found that nanocrystalline silver-derived solutions appear to have anti-inflammatory and prohealing activity, predominantly with a starting pH of 9. Solutions has been generated differently having various silver species with varying concentrations, only some of which are anti-inflammatory.…”

“…Solutions has been generated differently having various silver species with varying concentrations, only some of which are anti-inflammatory. 100 These Figure 5 Role of the Different Cytokines and Mediators at the Various Stages of Normal Wound Healing. ILGF-1, insulin-like growth factor 1; KGF, keratinocyte growth factor; PDGF, platelet-derived growth factor; VEGF, vascular endothelial growth factor; TGF-b 3, transforming growth factor beta 3; TGF-a, transforming growth factor alpha; HBEGF, heparin-binding epidermal growth factor; BFGF, basic fibroblast growth factor; AFGF, acidic fibroblast growth factor; TGF-b 1, 2, transforming growth factor beta 1, 2; TNF-a, tumor necrosis factor alpha; IL-1, -10, interleukin 1, 10; CSF-1, colony stimulating factor 1.…”

Synthesis of silver nanoparticles by using tea leaf extract from Camellia Sinensis

“…Silver nanoparticles are well known as one of the most universal antimicrobial substances in the field of biology and medicine due to their strong biocidal effect against microbial species, which has been used for centuries to prevent and treat various diseases, most notably infections. 14 Silver nanoparticles also reported to possess anti-fungal, 15 anti-inflammatory, 16 anti-viral, 17 anti-angiogenesis 18 and anti-platelet activity. 19 Recently, the development of silver nanoparticles is expanding.…”

Hybrid Amniotic Membrane Dressing with Green Silver Nanoparticles as Bioengineered Skin for Wounds and Burns: A Pilot Studies

“…The eco-friendly biosynthesis of silver nanoparticles using curcumin makes them superior wound healing product with enhanced biofilm activity [22][23][24]. Nano silver nitrate incorporated hybrid hAM bandage increases their manageability, provides the easier application to the burned area and creates a bactericidal effect, therefore reducing the risk of contamination and infection.…”