Anti-inflammatory activities of arthropod peptides: a systematic review

Santos, Ariane Teixeira dos; Cruz, Gabriela Silva; Baptista, Gandhi Rádis

doi:10.1590/1678-9199-jvatitd-2020-0152

Cited by 6 publications

(4 citation statements)

References 157 publications

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“…However, using viable counts to enumerate the colony-forming units after treatment with the AMPs would have allowed differentiation between bacteriostatic and bactericidal properties of the AMPs, which will be important for the characterization of purified AMP products. AMPs from mammalian sources and insects are naturally anti-inflammatory in addition to their antimicrobial role [38,39]. All of the 117 'potent' putative AMPs were predicted to be potentially anti-inflammatory, with more than 90% having a probability above 0.6 (Table S2).…”

Section: Discussionmentioning

confidence: 99%

In Silico and In Vitro Analyses Reveal Promising Antimicrobial Peptides from Myxobacteria

Arakal

Whitworth

James

et al. 2022

Probiotics & Antimicro. Prot.

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Antimicrobial resistance (AMR) is a global concern, and as soon as new antibiotics are introduced, resistance to those agents emerges. Therefore, there is an increased appetite for alternative antimicrobial agents to traditional antibiotics. Here, we used in silico methods to investigate potential antimicrobial peptides (AMPs) from predatory myxobacteria. Six hundred seventy-two potential AMP sequences were extracted from eight complete myxobacterial genomes. Most putative AMPs were predicted to be active against Klebsiella pneumoniae with least activity being predicted against Staphylococcus aureus. One hundred seventeen AMPs (defined here as ‘potent putative AMPs’) were predicted to have very good activity against more than two bacterial pathogens, and these were characterized further in silico. All potent putative AMPs were predicted to have anti-inflammatory and antifungal properties, but none was predicted to be active against viruses. Twenty six (22%) of them were predicted to be hemolytic to human erythrocytes, five were predicted to have anticancer properties, and 56 (47%) were predicted to be biofilm active. In vitro assays using four synthesized AMPs showed high MIC values (e.g. So_ce_56_913 250 µg/ml and Coral_AMP411 125 µg/ml against E. coli). However, antibiofilm assays showed a substantial reduction in numbers (e.g. Coral_AMP411 and Myxo_mac104 showed a 69% and 73% reduction, respectively, at the lowest concentration against E. coli) compared to traditional antibiotics. Fourteen putative AMPs had high sequence similarity to proteins which were functionally associated with proteins of known function. The myxobacterial genomes also possessed a variety of biosynthetic gene clusters (BGCs) that can encode antimicrobial secondary metabolites, but their numbers did not correlate with those of the AMPs. We suggest that AMPs from myxobacteria are a promising source of novel antimicrobial agents with a plethora of biological properties.

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Section: Discussionmentioning

confidence: 99%

In Silico and In Vitro Analyses Reveal Promising Antimicrobial Peptides from Myxobacteria

Arakal

Whitworth

James

et al. 2022

Probiotics & Antimicro. Prot.

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“…AMPs from mammalian sources and insects are naturally anti-in ammatory in addition to their antimicrobial role [38,39]. All of the 117 'potent' putative AMPs, were predicted to be potentially anti-in ammatory, with more than 90% having a probability above 0.6 (Table S2).…”

Section: Discussionmentioning

confidence: 99%

Insilico and Invitro Analyses Reveal Promising Antimicrobial Peptides from Myxobacteria

Arakal

Whitworth²,

James

et al. 2022

Preprint

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Antimicrobial resistance (AMR) is a global concern, and as soon as new antibiotics are introduced, resistance to those agents emerges. Therefore, there is an increased appetite for alternative antimicrobial agents to traditional antibiotics. Here, we used in silico methods to investigate potential antimicrobial peptides (AMPs) from predatory myxobacteria. 672 potential AMP sequences were extracted from eight complete myxobacterial genomes. Most putative AMPs were predicted to be active against Klebsiella pneumoniae with least activity being predicted against Staphylococcus aureus. 117 AMPs (defined here as ‘potent putative AMPs’) were predicted to have very good activity against more than two bacterial pathogens and these were characterized further in silico. All potent putative AMPs were predicted to have anti-inflammatory and antifungal properties, but none were predicted to be active against viruses. 26 (22%) of them were predicted to be hemolytic to human erythrocytes, five were predicted to have anticancer properties and 56 (47%) were predicted to be biofilm active. Invitro assays using four synthesized AMPs showed promising antibiofilm activities despite high MIC values. 14 putative AMPs had high sequence similarity to proteins which were functionally associated with proteins of known function. The myxobacterial genomes also possessed a variety of biosynthetic gene clusters (BGCs) that can encode antimicrobial secondary metabolites, but their numbers did not correlate with those of the AMPs. We suggest that AMPs from myxobacteria are a promising source of novel antimicrobial agents with a plethora of biological properties.

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“…One of the remarkable aspects of animal peptides is the diversity in their sources and structures. Venomous creatures, such as snakes, spiders [40,41], and cone snails, are well-known for producing peptides with potent bioactivities, including antinociceptive effects [42]. Additionally, peptides derived from insects or the skin secretions of amphibians have also shown promise in pain modulation.…”

Section: Antinociceptive Peptides Of Animal Originmentioning

confidence: 99%

Analgesic Peptides: From Natural Diversity to Rational Design

Gach-Janczak,

Biernat,

Kuczer

et al. 2024

Molecules

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Pain affects one-third of the global population and is a significant public health issue. The use of opioid drugs, which are the strongest painkillers, is associated with several side effects, such as tolerance, addiction, overdose, and even death. An increasing demand for novel, safer analgesic agents is a driving force for exploring natural sources of bioactive peptides with antinociceptive activity. Since the G protein-coupled receptors (GPCRs) play a crucial role in pain modulation, the discovery of new peptide ligands for GPCRs is a significant challenge for novel drug development. The aim of this review is to present peptides of human and animal origin with antinociceptive potential and to show the possibilities of their modification, as well as the design of novel structures. The study presents the current knowledge on structure-activity relationship in the design of peptide-based biomimetic compounds, the modification strategies directed at increasing the antinociceptive activity, and improvement of metabolic stability and pharmacodynamic profile. The procedures employed in prolonged drug delivery of emerging compounds are also discussed. The work summarizes the conditions leading to the development of potential morphine replacements.

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Anti-inflammatory activities of arthropod peptides: a systematic review

Cited by 6 publications

References 157 publications

In Silico and In Vitro Analyses Reveal Promising Antimicrobial Peptides from Myxobacteria

In Silico and In Vitro Analyses Reveal Promising Antimicrobial Peptides from Myxobacteria

Insilico and Invitro Analyses Reveal Promising Antimicrobial Peptides from Myxobacteria

Analgesic Peptides: From Natural Diversity to Rational Design

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