Anti-Hyperglycemic Effect of Tecomella undulata Extract by Ameliorating Pancreatic Dysfunction in Streptozotocin Induced Diabetic Albino Rats

Abstract

The primary objective of the present investigation is to evaluate the antihyperglycemic activity of the 50% ethanolic extract of the Tecomella undulata Seem. (TUE) bark in the streptozotocin induced diabetic rats. Methods: Single intraperitoneal injection (i.p.) of streptozotocin (60 mg/kg body weight) was used for induction of diabetes is wistar albino rats. The induction of diabetes was confirmed after 3 days after STZ injection and rats with fasting blood glucose levels greater than 200 mg/dl were considered to be diabetic used in the experiment. TUE at a once a daily dose of 250 mg/kg, and 500 mg/kg, p.o. along with glibenclamide 600µg/kg, p.o. was also given for 21 days. On the 21 st day rats from all the groups fasted overnight and the blood was collected from the puncturing the retro orbit of the eye under mild ether anesthetic condition. The oral acute toxicity studies did not show any toxic effect till the dose at 2000 mg/kg. Results: The statistical data indicated that the different doses of the TUE significantly decreases the level of fasting blood glucose, total cholesterol, triglycerides, low density lipoprotein and increase high density lipoprotein in STZ induced diabetic rats. This result indicated that T. undulata extract can protect pancreatic βcells from STZ-induced damage which is confirmed by the results of the histopathological examination of the pancreas. Conclusion: Our investigation has clearly indicated that the bark extract of Tecomella undulata Seem. showed remarkable antihyperglycemic activity due to its possible systematic effect involving in the pancreatic and extra pancreatic mechanism. Moreover, the antihyperlipidemic activity was exerted possible by lowering the higher level of lipid profile.

Antidiabetic and antihyperlipidemic activity of Euphorbia thymifolia L. extracts on streptozotocin-nicotinamide induced type 2 diabetic rats

“…Reitman and Frankel (1957) were suggested method for estimation of hepatic amino transferase enzymes which includes aspartate amino transferase (AST or SGOT) and alanine aminotransferase (ALT or SGPT). The serum total cholesterol (TC), triglyceride (TG), low density lipoproteins (LDL) and high density lipoproteins (HDL) concentrations were determined using commercial kits by enzymatic photocolorimetric methods (Das, et al, 2015) …”

In-Silico Prediction of Phytoconstituents from Manilkara Hexandra for Antidiabetic Activity Targeting GKRP (Glucokinase Regulatory Protein)

“…According to the study's findings, 50% of the ethanolic extract exhibits considerable antidiabetic activity and a robust hypolipidemic potential in diabetic circumstances at various doses. (30) This research backs up our in-silico research that suggests quercetin and kaempferol, which have the lowest binding energies (-9.3 kcal/mol and -9.1 kcal/ mol, respectively) in complex with GKRP, may be useful in the treatment of diabetes. Regardless of the makeup of the phytoconstituents, the previously reported activity, however, is consistent with the extract's overall activity.…”

Histopathological effects with marwar teak (tecomella undulata) bark extract and N-acetylcysteine on acetaminophen induced hepatotoxicity in albino rats.

“…Then it was filtrated and extracted on rota vapour. 14 Acute toxicity test of other studies found 200 mg/ Kg of Tecomalla bark extract to be the safe dose. 15 All experimental animals were divided into four groups.…”