Anti‐fatigue activity of sea cucumber peptides prepared from <i>Stichopus japonicus</i> in an endurance swimming rat model

Ye, Jing; Shen, Caihong; Huang, Yayan; Zhang, Xueqin; Xiao, Mingqing

doi:10.1002/jsfa.8322

Cited by 58 publications

(43 citation statements)

References 54 publications

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“…All mice ran on the treadmill at 10 m/min for 10 min, followed by 16 m/min for 10 min and 21 m/min for 10 min once a week for three weeks. The time to exhaustion for exercise (10 m/min for 5 min, followed by 16,18,21,24,26,29,32,34 and 37 m/min for 3 min each, and 40 m/min until exhaustion) was determined on the 21st day. The time to exhaustion during treadmill running was defined as the time between the commencement and the first failure to maintain treadmill exercise for >3 min (24).…”

Section: Methodsmentioning

confidence: 99%

“…Leucine has important biochemical functions in the brain, such as the synthesis of dopamine, which is derived from aromatic amino acids, and leucine supplementation limits the development of fatigue (6). In addition, glycine-rich sea cucumber peptides were reported to exhibit an anti-fatigue activity (21). Therefore, the present study investigated the anti-fatigue effects of Danish porcine placenta (DPP) and its dipeptides, leucine-glycine (LG) and glycine-leucine (GL), in mouse models of exercise-induced fatigue using a treadmill test and a forced swimming test (FST), which are useful for screening anti-fatigue drugs (22,23).…”

Section: Introductionmentioning

confidence: 99%

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Leucine and glycine dipeptides of porcine placenta ameliorate physical fatigue through enhancing dopaminergic systems

Han

Kim

et al. 2017

Mol Med Report

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Fatigue is a common and serious health problem, and various dietary interventions have previously been employed to ameliorate fatigue. The aim of the current study was to investigate the anti‑fatigue effects of Danish porcine placenta (DPP) and its major dipeptides, including leucine‑glycine (LG) and glycine‑leucine (GL). The anti‑fatigue effects of orally administered DPP, LG and GL were determined using a treadmill exercise test and a forced swimming test (FST) in mice. Additionally, the anti‑inflammatory effects of DPP, LG and GL were investigated in activated splenocytes. The results demonstrated that oral treatment of mice with DPP, LG and GL increased the time to exhaustion during treadmill exercise. Furthermore, DPP, LG and GL enhanced the levels of dopamine, brain‑derived neurotrophic factor and phosphorylated-extracellular signal‑regulated kinase in the brains of mice with treadmill exercise‑induced exhaustive fatigue, and decreased levels of certain proinflammatory cytokines in the serum and spleen, as determined by ELISA and western blot analysis. Following treadmill exercise, commercial kits were employed to demonstrate that DPP, LG and GL reduced the levels of lactate dehydrogenase, lactate, creatine kinase, blood urea nitrogen, alanine transaminase and aspartate transaminase in the muscle and/or serum of mice. In addition, DPP, LG and GL enhanced the muscle and liver glycogen levels, catalase activity in the liver and serum superoxide dismutase activity. DPP, LG and GL also increased the proliferation of splenocytes and inhibited proinflammatory cytokine production by reducing the activation of caspase‑1 and nuclear factor‑κB in activated splenocytes, as determined by MTT assays, ELISA and western blotting, respectively. Furthermore, DPP, LG and GL reduced immobility time in the FST in mice. In conclusion, DPP may limit intensive exercise‑induced fatigue by increasing dopaminergic systems and inhibiting inflammatory responses.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Leucine and glycine dipeptides of porcine placenta ameliorate physical fatigue through enhancing dopaminergic systems

Han

Kim

et al. 2017

Mol Med Report

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“…Thus far, the preparation and characterization of collagen from Parastichopus californicus [13], Stichopus vastus [14], Holothuria parva [15], Stichopus japonicus [16], Stichopus monotuberculatus [17], and Acaudina leucoprocta [18] have been reported. Furthermore, bioactive peptides produced by the hydrolysis of sea cucumber collagen have also been extensively studied [19][20][21]. One highly valued sea cucumber, Acaudina molpadioides, is widely distributed at the sandy bottoms of the East China Sea.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical Properties of Collagen from Acaudina Molpadioides and Its Protective Effects against H2O2-Induced Injury in RAW264.7 Cells

et al. 2020

Marine Drugs

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Collagen is a promising biomaterial used in the beauty and biomedical industries. In this study, the physicochemical characterization, antioxidant activities, and protective effects against H2O2-induced injury of collagen isolated from Acaudina molpadioides were investigated. The amino acid composition analysis showed that the collagen was rich in glycine (Gly), alanine (Ala), and glutamic acid (Glu), but poor in tyrosine (Tyr) and phenylalanine (Phe). Zeta potential analysis revealed that the isoelectric point (pI) of collagen from Acaudina molpadioides was about 4.25. It possessed moderate scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals in a dose-dependent manner. In addition, the collagen was able to effectively improve cell viability and morphology, inhibit the production of Malondialdehyde (MDA), and increase the activities of Superoxide Dismutase (SOD) and Glutathione Peroxidase (GSH-Px) in cultured RAW264.7 cells, resulting in a protective effect against H2O2-induced injury. Overall, the results showed that collagen extracted from A. molpadioides has promising prospects in the beauty and cosmetics industries.

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“…Excessive energy consumption and the accumulation of metabolic products as a result of physical and mental stress may cause irreversible oxidative tissue damage and lead to depressed immunity, accelerated ageing and cardio-cerebrovascular disease (28,29). The present study identified the anti-fatigue properties of TM in a mouse model by performing forced swimming, rotary rod and exhausted running tests, which are considered to be valid methods of evaluating the exercise capacity of mice (30).…”

Section: Discussionmentioning

confidence: 93%

The anti‑fatigue activities of Tuber melanosporum in a mouse model

Jiang

Chu

et al. 2018

Exp Ther Med

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Abstract. Tuber melanosporum (TM) is an edible fungus that exhibits antioxidant and anti-tumor activity via its unique bioactive metabolites. The present study analyzed the anti-fatigue effects of TM using a BALB/c mouse model. The anti-fatigue properties of TM were evaluated by assessing the endurance of mice by performing forced swimming, rotary rod and running tests. Following 2 weeks TM treatment, hepatic and muscular ATP, and glycogen levels were increased in mice subjected to 30 min swimming, compared with controls. Similarly, levels of serum lactic acid and lactic dehydrogenase were decreased in the same group, compared with the control. Additionally, TM treatment reduced reactive oxygen species and malondialdehyde levels, and increased superoxide dismutase and glutathione peroxidase levels in the muscle, liver and/or serum. The effect of TM on hormone levels was also investigated in the present study, as different efficacies of TM were observed in male and female mice. TM treatment increased serum levels of progesterone, estradiol and testosterone in female and male mice, whereas a decrease in serum luteinizing hormone levels was only observed in females. A decrease in serum follicle-stimulating hormone levels was identified in females, whereas an increase was observed in males. The current study demonstrated that the anti-fatigue effects of TM occur via the regulation of oxidative stress, energy metabolism and hormone levels.

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Anti‐fatigue activity of sea cucumber peptides prepared from Stichopus japonicus in an endurance swimming rat model

Abstract: SCP has anti-fatigue activity and it exerted its anti-fatigue effect probably through normalizing energy metabolism as well as alleviating oxidative damage and inflammatory responses. © 2017 Society of Chemical Industry.

Cited by 58 publications

References 54 publications

Leucine and glycine dipeptides of porcine placenta ameliorate physical fatigue through enhancing dopaminergic systems

Leucine and glycine dipeptides of porcine placenta ameliorate physical fatigue through enhancing dopaminergic systems

Physicochemical Properties of Collagen from Acaudina Molpadioides and Its Protective Effects against H2O2-Induced Injury in RAW264.7 Cells

The anti‑fatigue activities of Tuber melanosporum in a mouse model

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