Physicochemical Properties of Collagen from Acaudina Molpadioides and Its Protective Effects against H2O2-Induced Injury in RAW264.7 Cells

Abstract

Collagen is a promising biomaterial used in the beauty and biomedical industries. In this study, the physicochemical characterization, antioxidant activities, and protective effects against H2O2-induced injury of collagen isolated from Acaudina molpadioides were investigated. The amino acid composition analysis showed that the collagen was rich in glycine (Gly), alanine (Ala), and glutamic acid (Glu), but poor in tyrosine (Tyr) and phenylalanine (Phe). Zeta potential analysis revealed that the isoelectric point (pI) of collagen from Acaudina molpadioides was about 4.25. It possessed moderate scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals in a dose-dependent manner. In addition, the collagen was able to effectively improve cell viability and morphology, inhibit the production of Malondialdehyde (MDA), and increase the activities of Superoxide Dismutase (SOD) and Glutathione Peroxidase (GSH-Px) in cultured RAW264.7 cells, resulting in a protective effect against H2O2-induced injury. Overall, the results showed that collagen extracted from A. molpadioides has promising prospects in the beauty and cosmetics industries.

Collagen peptides from Acaudina molpadioides prevent CCl4‐induced liver injury via Keap1/Nrf2‐ARE, PI3K/AKT, and MAPKs pathways

“…On the contrary, the contents of positively charged amino acids (histidine [His] and lysine [Lys]) and hydrophobic amino acids (methionine [Met], tyrosine [Tyr], and phenylalanine [Phe]) were very low. The amino acid compositions of AMP were consistent with those of collagen obtained from A. molpadioides (J. Li et al., 2020). It was reported that Met, Tyr, His, and Cys (cysteine) could enhance the antioxidant activities of peptides (Ao & Li, 2012).…”

“…Collagen of A. molpadioides was obtained according to the method reported in our previous study (J. Li et al., 2020). The collagen was added into a buffer solution (pH = 10.0, w/v = 1/100) and pretreated for 30 min under microwave irradiation of 250 W. The mixtures were hydrolyzed at 45°C for 1 h by alkaline protease (≥200 U/mg).…”

“…Collagen of A. molpadioides was obtained according to the method reported in our previous study (J. Li et al, 2020).…”

Acaudina molpadioides mediates lipid uptake by suppressing PCSK9 transcription and increasing LDL receptor in human liver cells

“… Compound / metabolites Biological activities References Cerebroside Reduces monosaturated fatty acid biosynthesis Hu et al, 2014a , Hu et al, 2014b Cerebroside Increases insulin sensitivity, reduces serum and hepatic LDL level, decreases atherosclerotic lesion formation and attenuates inflammation by decreasing the levels of inflammatory cytokines, such as CRP, TNFα, IL-6 Zhang et al, 2018 Fucosylated polysaccharide sulfate Anticoagulant activity Dong et al, 2014 Fucoidan Attenuates TGF-β1 signal which associated with the improvement against hyperglycemia, obesity, oxidative stress and inflammation Hu et al, 2017 Peptides Anti-angiotensin converting enzyme (ACE) activity Jiang et al, 2018 Fucosylated chondroitin sulfate Promotes antiadipogenic activity by increasing β-catenin, a negative regulator in adipogenesis, and decreases the expression of SREBP-1c, PPARγ and C/EBPα. Xu et al, 2015 Fucoidan Exhibits antiadipogenic activity by modulating the Wnt/β-catenin pathway and downregulating the expression of SREBP-1c PPARγ and C/EBPα, while SREBP-1c Xu et al, 2014 Fucoidan Antihypertensive activity Zhao et al, 2009 Fucosylated chondroitin sulphate Mitigates hyperglycaemia /insulin resistance by increasing glucose uptake and GLUT4 translocation Hu et al, 2014b Cerebroside Ameliorates nonalcoholic fatty liver disease (NAFLD) through suppression of stearoyl-CoA desaturase activity, reduces hepatic triglyceride (TG) and total cholesterol (TC) levels Xu et al, 2011 Collagen Antioxidant activity Li et al, 2020 Cerebroside Antitumor activity by inducing apoptosis Du et al, 2012 Collagen Potent antioxidant activity by preventing the injury of nerve cells induced by hydrogen peroxide Yu and Chen, 2014 …”

Protective and Anti-Aging Effects of 5 Cosmeceutical Peptide Mixtures on Hydrogen Peroxide-Induced Premature Senescence in Human Skin Fibroblasts

“…GSH-Px converts reduced glutathione to oxidized glutathione while reducing lipid hydroperoxides to their corresponding alcohols or free hydrogen peroxide to water [25]. Figure 5b shows that the GSH-Px activity in the H 2 O 2 group was significantly lower than that of the blank control group but increased significantly after pretreatment with those peptide mixtures.…”