Anti-dopaminergic effect of the methanolic extract of Morus alba L. leaves

Yadav, Adhikrao Vyankatrao; Nade, Vandana S

doi:10.4103/0253-7613.44154

Cited by 32 publications

(14 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using those findings, we characterized 1-3 as D 1 R/D 2L R antagonists. In a previous study, M. alba leaf extract possessed D 2 R-mediated anti-dopaminergic activity, suggesting a possible clinical application for M. alba leaves in psychiatric disorders [46]. Our findings suggest that 1-3 could have antipsychotic effects.…”

Section: Discussionsupporting

confidence: 58%

Novel Diels–Alder Type Adducts from Morus alba Root Bark Targeting Human Monoamine Oxidase and Dopaminergic Receptors for the Management of Neurodegenerative Diseases

Paudel

Park

Seong

et al. 2019

IJMS

View full text Add to dashboard Cite

In this study, we delineate the human monoamine oxidase (hMAO) inhibitory potential of natural Diels–Alder type adducts, mulberrofuran G (1), kuwanon G (2), and albanol B (3), from Morus alba root bark to characterize their role in Parkinson’s disease (PD) and depression, focusing on their ability to modulate dopaminergic receptors (D1R, D2LR, D3R, and D4R). In hMAO-A inhibition, 1–3 showed mild effects (50% inhibitory concentration (IC50): 54‒114 μM). However, 1 displayed moderate inhibition of the hMAO-B isozyme (IC50: 18.14 ± 1.06 μM) followed by mild inhibition by 2 (IC50: 57.71 ± 2.12 μM) and 3 (IC50: 90.59 ± 1.72 μM). Our kinetic study characterized the inhibition mode, and the in silico docking predicted that the moderate inhibitor 1 would have the lowest binding energy. Similarly, cell-based G protein-coupled receptors (GPCR) functional assays in vector-transfected cells expressing dopamine (DA) receptors characterized 1–3 as D1R/D2LR antagonists and D3R/D4R agonists. The half-maximum effective concentration (EC50) of 1–3 on DA D3R/D4R was 15.13/17.19, 20.18/21.05, and 12.63/‒ µM, respectively. Similarly, 1–3 inhibited 50% of the DA response on D1R/D2LR by 6.13/2.41, 16.48/31.22, and 7.16/18.42 µM, respectively. A computational study revealed low binding energy for the test ligands. Interactions with residues Asp110, Val111, Tyr365, and Phe345 at the D3R receptor and Asp115 and His414 at the D4R receptor explain the high agonist effect. Likewise, Asp187 at D1R and Asp114 at D2LR play a crucial role in the antagonist effects of the ligand binding. Our overall results depict 1–3 from M. alba root bark as good inhibitors of hMAO and potent modulators of DA function as D1R/D2LR antagonists and D3R/D4R agonists. These active constituents in M. alba deserve in-depth study for their potential to manage neurodegenerative disorders (NDs), particularly PD and psychosis.

show abstract

Section: Discussionsupporting

confidence: 58%

Novel Diels–Alder Type Adducts from Morus alba Root Bark Targeting Human Monoamine Oxidase and Dopaminergic Receptors for the Management of Neurodegenerative Diseases

Paudel

Park

Seong

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…Brain dopamine level has been reported to increase in FSIA. [ 20 ] The 0.8 mA current was given to the animals consecutively for 7 days. Pretreatment with B. vulgaris (300 mg/kg) for 7 days significantly increased the number of fighting attacks, therefore suggesting a possible dopaminergic activity of B. vulgaris in FSIA.…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective potential of Beta vulgaris L. in Parkinson′s disease

Nade¹,

Kawale²,

Zambre³

et al. 2015

Indian J Pharmacol

Self Cite

View full text Add to dashboard Cite

Objective:The objective was to investigate the neuroprotective role of Beta vulgaris in Parkinson's disease (PD).Materials and Methods:PD was induced by administration of reserpine (5 mg/kg/day, i.p for 5 consecutive days), haloperidol (1 mg/kg, i.p.), and tacrine (2.5 mg/kg, i.p.) in experimental animals. The symptoms of PD such as tremors, akinesia, rigidity, catalepsy, and vacuous chewing movements (VCMs) were evaluated. Foot shock-induced aggression (FSIA) model was used to confirm anti-parkinsonian activity. The methanolic extract of Beta vulgaris (MEBV) was administered at doses of 100, 200, and 300 mg/kg, p.o. The combination of L-dopa and carbidopa was used as a standard drug. Behavioral studies such as locomotor activity and grip strength were determined, and oxidative stress was evaluated in FSIA model in rat brain.Results:Pretreatment with MEBV (200 and 300 mg/kg) significantly reduced the intensity of muscular rigidity, duration of catalepsy, akinesia, the number of tremors, VCMs, and increase fighting behavior. The locomotor activity and grip strength were significantly increased by MEBV. In FSIA, the biochemical analysis of brain revealed the increased level of lipid peroxidation (LPO) and decreased levels of superoxide dismutase (SOD) and catalase (CAT). MEBV significantly reduced LPO level and restored the defensive antioxidant enzymes SOD and CAT in rat brain.Conclusions:The results indicated the protective role of B. vulgaris against PD. The mechanism of protection may be due to augmentation of cellular antioxidants.

show abstract

“…The increase in BUN and serum creatinine and reduction in urinary creatinine is due to the disturbance in the out flow of urine. The result impaired GFR 14 . Treatment with extract significantly decreased the plasma creatinine, BUN and increased the urine creatinine levels.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Anti-Nephrolithiatic Activity of Ethanolic Leaf Extract of Morus Alba L. In Animal Models

S¹,

Pramod²

2014

Int. Res. J. Pharm.

View full text Add to dashboard Cite

Kidney stone is a common painful condition of multifactorial etiopathogenesis, and calcium oxalate (CaOx) is the commonest component of stone. The effect of the ethanolic leaf extract of Morus alba L. against Calculi-Producing Diet induced nephrolithiasis in Wistar rats is summarized in the present study. Nephrolithiasis was induced by fed with Calculi-Producing Diet (CPD: Rat pellet mixed with 5 % sodium oxalate) and it was manifested by high calcium, oxalate, serum creatinine, Blood Urea Nitrogen, reduction in urine volume and creatinine clearance. The extraction was done by successive cold maceration and evaporation by distillation using 95 % ethanol. After the extraction the crude extract were subjected to phytochemical screening. The preliminary phytochemical screening showed the presence of alkaloids, glycosides, flavonoids, phenols, tannins, carbohydrates, proteins, saponins and sterols. The extract of Morus alba L. showed a potent percentage inhibition in different in vitro models.The treatment with plant extract (500 and 1000 mg/kg/day), exhibited a significant effect in preventing calcium oxalate formation and also in dissolving the preformed stones. Significant effect is exhibited by the treatment groups with 1000 mg/kg/day. Histopathological evaluation also supports the protective effect of Morus alba L.

show abstract

Anti-dopaminergic effect of the methanolic extract of Morus alba L. leaves

Cited by 32 publications

References 19 publications

Novel Diels–Alder Type Adducts from Morus alba Root Bark Targeting Human Monoamine Oxidase and Dopaminergic Receptors for the Management of Neurodegenerative Diseases

Novel Diels–Alder Type Adducts from Morus alba Root Bark Targeting Human Monoamine Oxidase and Dopaminergic Receptors for the Management of Neurodegenerative Diseases

Neuroprotective potential of Beta vulgaris L. in Parkinson′s disease

Evaluation of Anti-Nephrolithiatic Activity of Ethanolic Leaf Extract of Morus Alba L. In Animal Models

Contact Info

Product

Resources

About