Anti-Biofilm Effects of Synthetic Antimicrobial Peptides Against Drug-Resistant Pseudomonas aeruginosa and Staphylococcus aureus Planktonic Cells and Biofilm

Park, Seong-Cheol; Lee, Min Young; Kim, Jin‐Young; Kim, Hyeonseok; Jung, Myunghwan; Shin, Min-Kyoung; Cheong, Gang-Won; Lee, Jung Ro; Jang, Mi-Kyeong

doi:10.3390/molecules24244560

Cited by 37 publications

(22 citation statements)

References 39 publications

(54 reference statements)

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“…The combined use of a biofilm inhibitor and an antibiotic causes a certain degree of damage to the biofilm and increases the opportunity for the antibiotic to contact the bacteria in the biofilm, resulting in synergistic antibacterial activity and the destruction of the biofilm [ 56 , 57 ]. APEC biofilms on the slides were not destroyed after treatment with florfenicol alone at the MIC (64 μg/mL).…”

Section: Discussionmentioning

confidence: 99%

In vitro antibiofilm activity of resveratrol against avian pathogenic Escherichia coli

et al. 2021

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Background Avian pathogenic Escherichia coli (APEC) strains cause infectious diseases in poultry. Resveratrol is extracted from Polygonum cuspidatum, Cassia tora Linn and Vitis vinifera, and displays good antimicrobial activity. The present study aimed to investigate the antibiofilm effect of resveratrol on APEC in vitro. The minimum inhibitory concentration (MIC) of resveratrol and the antibiotic florfenicol toward APEC were detected using the broth microdilution method. Then, the effect of resveratrol on swimming and swarming motility was investigated using a semisolid medium culture method. Subsequently, the minimum biofilm inhibitory concentration (MBIC) and the biofilm eradication rate were evaluated using crystal violet staining. Finally, the antibiofilm activity of resveratrol was observed using scanning electron microscopy (SEM). Meanwhile, the effects of florfenicol combined with resveratrol against biofilm formation by APEC were evaluated using optical microscopy (OM) and a confocal laser scanning microscopy (CLSM). Results The MICs of resveratrol and florfenicol toward APEC were 128 μg/mL and 64 μg/mL, respectively. The swimming and swarming motility abilities of APEC were inhibited in a resveratrol dose-dependent manner. Furthermore, resveratrol showed a significant inhibitory activity against APEC biofilm formation at concentrations above 1 μg/mL (p < 0.01). Meanwhile, the inhibitory effect of resveratrol at 32 μg/mL on biofilm formation was observed using SEM. The APEC biofilm was eradicated at 32 μg/mL of resveratrol combined with 64 μg/mL of florfenicol, which was observed using CLSM and OM. Florfenicol had a slight eradication effect of biofilm formation, whereas resveratrol had a strong biofilm eradication effect toward APEC. Conclusion Resveratrol displayed good antibiofilm activity against APEC in vitro, including inhibition of swimming and swarming motility, biofilm formation, and could eradicate the biofilm.

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Section: Discussionmentioning

confidence: 99%

In vitro antibiofilm activity of resveratrol against avian pathogenic Escherichia coli

et al. 2021

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“…Natural or synthetic AMP sequences with positively charged residues (arginine or lysine) and their amphipathic structure can express activity against a broad spectrum of both Gram-positive and Gram-negative bacteria and are not prone to resistance development, as they act against multiple targets in the bacteria (Hale and Hancock 2007;Sang and Blecha 2008;Torcato et al 2013). The AMPs can be active against planktonic living bacteria and/or show anti-biofilm properties, for example, inhibit biofilm formation or eradicate mature biofilms (de la Fuente-Nunez et al 2013; de la Fuente-Nunez et al 2016;Park et al 2019; Der Torossian Torres and de la Fuente-Nunez 2019). Remarkably, there is not necessarily a correlation between the anti-planktonic and antibiofilm effectiveness of AMPs (de la Fuente-Nunez et al 2013; Der Torossian Torres and de la Fuente-Nunez 2019).…”

Section: Introductionmentioning

confidence: 99%

“…2016; Park et al . 2019; Der Torossian Torres and de la Fuente‐Nunez 2019). Remarkably, there is not necessarily a correlation between the anti‐planktonic and anti‐biofilm effectiveness of AMPs (de la Fuente‐Nunez et al .…”

Section: Introductionmentioning

confidence: 99%

Synthetic anti‐endotoxin peptides interfere with Gram‐positive and Gram‐negative bacteria, their adhesion and biofilm formation on titanium

Subh

Correa

Pinkvos

et al. 2020

J. Appl. Microbiol.

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Aims: Implant-associated infections arise from the formation of bacterial biofilms, which are difficult to be treated with conventional antibiotics. Therefore, there is a need for new implant functionalizations, which inhibit biofilm formation. The aim of the present study was to characterize the effect of synthetic peptides to assess their applicability for this purpose. Methods and Results: Two synthetic anti-endotoxin peptides, Pep19-2.5 and Pep19-4LF (Aspidasept I and II) were tested against both Gram-positive (Staphylococcus aureus and Streptococcus oralis) and Gram-negative (Pseudomonas aeruginosa and Aggregatibacter actinomycetemcomitans) bacteria associated with implant infections. Their activity was evaluated against different states of biofilm formation on the implant material titanium using CFU, live/dead fluorescence staining and confocal microscopy. Both peptides inhibited planktonic bacteria growth, impacted initial bacterial adhesion, reduced biofilm volume and increased the proportion of dead cells. Additionally, cytotoxicity analyses showed that neither peptide harmed human gingival fibroblasts nor osteoblasts at lower concentrations. Conclusion: A concentration-dependent antibacterial activity of both peptides against biofilms of four clinically relevant bacteria could be demonstrated. Significance and Impact of the Study: The results of this study serve as a promising basis for the improvement of these peptides in order to finally achieve a peptide-equipped antibacterial implant surface.

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“…Similarly to other antibiofilm peptides against human pathogens ( Mishra and Guangshun, 2017 ; Park et al, 2019 ; Qi et al, 2020 ), the antibiofilm activity of BP525 , 1037 , and R-FV7-I16 showed a dose–effect relationship that fitted well with a Michaelis–Menten saturation curve. Interestingly, they showed low ED 50 values, which means that low peptide concentrations already display high antibiofilm activity.…”

Section: Discussionmentioning

confidence: 74%

Antimicrobial Peptides With Antibiofilm Activity Against Xylella fastidiosa

et al. 2021

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Xylella fastidiosa is a plant pathogen that was recently introduced in Europe and is causing havoc to its agriculture. This Gram-negative bacterium invades the host xylem, multiplies, and forms biofilm occluding the vessels and killing its host. In spite of the great research effort, there is no method that effectively prevents or cures hosts from infections. The main control strategies up to now are eradication, vector control, and pathogen-free plant material. Antimicrobial peptides have arisen as promising candidates to combat this bacterium due to their broad spectrum of activity and low environmental impact. In this work, peptides previously reported in the literature and newly designed analogs were studied for its bactericidal and antibiofilm activity against X. fastidiosa. Also, their hemolytic activity and effect on tobacco leaves when infiltrated were determined. To assess the activity of peptides, the strain IVIA 5387.2 with moderate growth, able to produce biofilm and susceptible to antimicrobial peptides, was selected among six representative strains found in the Mediterranean area (DD1, CFBP 8173, Temecula, IVIA 5387.2, IVIA 5770, and IVIA 5901.2). Two interesting groups of peptides were identified with bactericidal and/or antibiofilm activity and low-moderate toxicity. The peptides 1036 and RIJK2 with dual (bactericidal–antibiofilm) activity against the pathogen and moderate toxicity stand out as the best candidates to control X. fastidiosa diseases. Nevertheless, peptides with only antibiofilm activity and low toxicity are also promising agents as they could prevent the occlusion of xylem vessels caused by the pathogen. The present work contributes to provide novel compounds with antimicrobial and antibiofilm activity that could lead to the development of new treatments against diseases caused by X. fastidiosa.

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Anti-Biofilm Effects of Synthetic Antimicrobial Peptides Against Drug-Resistant Pseudomonas aeruginosa and Staphylococcus aureus Planktonic Cells and Biofilm

Cited by 37 publications

References 39 publications

In vitro antibiofilm activity of resveratrol against avian pathogenic Escherichia coli

In vitro antibiofilm activity of resveratrol against avian pathogenic Escherichia coli

Synthetic anti‐endotoxin peptides interfere with Gram‐positive and Gram‐negative bacteria, their adhesion and biofilm formation on titanium

Antimicrobial Peptides With Antibiofilm Activity Against Xylella fastidiosa

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