Anti-biofilm activity of Rosmarinus officinalis, Punica granatum and Tetradenia riparia against methicillin-resistant Staphylococcus aureus (MRSA) and synergic interaction with penicillin

Endo, Eliana Harue; G, Costa; Makimori, Regina Yasuko; Ueda-Nakamura, Tânia; Nakamura, Celso Vataru; Filho, Benedito Prado Dias

doi:10.1016/j.hermed.2018.07.001

Cited by 24 publications

(24 citation statements)

References 41 publications

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“…The MIC test was performed in a 96 well plate as described by Endo et al [100] with slight modification. Briefly, a 50 µL of bacterial suspension (1 × 10 6 CFU/mL) prepared in TSB media was loaded into each of the 96 well microplate except the negative control (TSB only).…”

Section: Mic and Mbc Of The Methanolic Extract Musc 125mentioning

confidence: 99%

Streptomyces sp. Strain MUSC 125 from Mangrove Soil in Malaysia with Anti-MRSA, Anti-Biofilm and Antioxidant Activities

et al. 2020

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There is an urgent need to search for new antibiotics to counter the growing number of antibiotic-resistant bacterial strains, one of which is methicillin-resistant Staphylococcus aureus (MRSA). Herein, we report a Streptomyces sp. strain MUSC 125 from mangrove soil in Malaysia which was identified using 16S rRNA phylogenetic and phenotypic analysis. The methanolic extract of strain MUSC 125 showed anti-MRSA, anti-biofilm and antioxidant activities. Strain MUSC 125 was further screened for the presence of secondary metabolite biosynthetic genes. Our results indicated that both polyketide synthase (pks) gene clusters, pksI and pksII, were detected in strain MUSC 125 by PCR amplification. In addition, gas chromatography-mass spectroscopy (GC-MS) detected the presence of different chemicals in the methanolic extract. Based on the GC-MS analysis, eight known compounds were detected suggesting their contribution towards the anti-MRSA and anti-biofilm activities observed. Overall, the study bolsters the potential of strain MUSC 125 as a promising source of anti-MRSA and antibiofilm compounds and warrants further investigation.

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Section: Mic and Mbc Of The Methanolic Extract Musc 125mentioning

confidence: 99%

Streptomyces sp. Strain MUSC 125 from Mangrove Soil in Malaysia with Anti-MRSA, Anti-Biofilm and Antioxidant Activities

et al. 2020

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“…The triterpene saponin glycyrrhetinic acid isolated from liquorice is a potent inhibitor of S. aureus alpha-haemolysin (Hla) at 1-8 µg/mL, concentrations well below its minimal inhibitory concentration (MIC) of >512 µg/mL and protects mice from S. aureus pneumonia [47]. Hydroalcoholic extracts of rosemary, similar to the extracts used in our study, have been shown to exhibit anti-MRSA biofilm activity as low as 20 µg/mL [48]. De Olivira et al have also found that rosemary extracts to be a safe and effective in controlling not only S. aureus, but also Candida albicans, Enterococcus faecalis, Streptococcus mutans, and Pseudomonas aeruginosa in both mono-and polymicrobial biofilms.…”

Section: Discussionmentioning

confidence: 63%

“…At these concentrations, there is little to no inhibition of bacterial growth. Endo et al measured the minimal inhibitory concentration (MIC) of a hydroalcoholic extract of R. officinalis for inhibition of various strains of MRSA in both planktonic and biofilm states [48]. Rosemary extracts showed MICs ranges from 15.6-62.5 µg/mL for planktonic growth and as low 45-250 µg/mL for preformed biofilms, depending on the MRSA isolate.…”

Section: Discussionmentioning

confidence: 99%

Rosmarinus officinalis L. (Rosemary) Extracts Containing Carnosic Acid and Carnosol are Potent Quorum Sensing Inhibitors of Staphylococcus aureus Virulence

2020

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Staphylococcus aureus is an opportunistic pathogen and a common cause of skin infection. S. aureus also plays a role in the pathogenesis of the chronic inflammatory skin disease, atopic dermatitis. S. aureus virulence involves activation of the quorum sensing agr operon. In this paper, we show that the diterpene carnosic acid, present in R. officinalis L. (rosemary) leaves, is a specific inhibitor of S. aureus agr expression as low as 5 μM. Carnosol and rosmarinic acid are two other phytochemicals present in rosemary leaves. Carnosol, but not rosmarinic acid, is also a potent agr expression inhibitor. Natural rosemary extracts containing carnosic acid and carnosol inhibit S. aureus agr expression, both in luciferase reporter strains and in wild type strains isolated from patients with atopic dermatitis. Specific inhibition of S. aureus virulence using topical formulations of rosemary extract may offer a practical approach to preventing and treating flares of atopic dermatitis.

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“…Los principales usos del romero son en cosméticos, saborizantes de comida (Basheer, 2018;Borges et al, 2018), mejoradores de la vida de anaquel en alimentos perecederos (Fidan et al, 2019;Said, Waheed & Khalifa, 2019), estimulantes del crecimiento del cabello, como aroma en la preparación de perfumes y fragancias (Endo et al, 2018), como aditivo para shampoo, crema y jabón (Akhbari, Masoum, Aghababaei & Hamedi, 2018), en colorantes naturales de lociones y enjuagues bucales (Gomes et al, 2020).…”

Section: Romero (R Officinalis L)unclassified

“…La obtención de extractos acuosos del romero mediante la maceración de sus hojas con agua destilada, requiere un adecuado control de la temperatura con el fin de evitar el crecimiento de microorganismos (Zoral et al, 2018). Se opta por usar una solución hidroalcohólica como agente extractor en las hojas del romero (Endo et al, 2018). También se trabaja con sus flores como materia prima (Karadağ et al, 2019).…”

Section: Extracción De Metabolitos Secundarios Del Romero (R Officinunclassified

Romero (Rosmarinus officinalis L.): su origen, importancia y generalidades de sus metabolitos secundarios

et al. 2020

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El presente trabajo, es una revisión que muestra el impacto que tiene el uso de las plantas medicinales en la salud humana. Desde la antigüedad, por tradición, se extendió el uso mayoritario de algunas familias vegetales como la Lamiaceae que destaca por sus propiedades aromáticas y aplicación culinaria en numerosas culturas, así como en el tratamiento y prevención de una gran variedad de enfermedades y malestares. Dentro de las especies pertenecientes a esta familia se encuentra el romero (Rosmarinus officinalis), planta que ha ganado importancia en el campo de la investigación por sus diversos atributos biológicos como: antiinflamatorio, antimicrobiano, antioxidante y anticancerígeno, entre otros; resultados que debe a sus metabolitos secundarios como: el ácido carnósico, el carnosol, el ácido rosmárico y el alcanfor, entre otros más, aunado a un potencial efecto cuando es aplicado. También se mencionan algunas metodologías que buscan la extracción de los componentes biológicamente activos del romero.

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Anti-biofilm activity of Rosmarinus officinalis, Punica granatum and Tetradenia riparia against methicillin-resistant Staphylococcus aureus (MRSA) and synergic interaction with penicillin

Cited by 24 publications

References 41 publications

Streptomyces sp. Strain MUSC 125 from Mangrove Soil in Malaysia with Anti-MRSA, Anti-Biofilm and Antioxidant Activities

Streptomyces sp. Strain MUSC 125 from Mangrove Soil in Malaysia with Anti-MRSA, Anti-Biofilm and Antioxidant Activities

Rosmarinus officinalis L. (Rosemary) Extracts Containing Carnosic Acid and Carnosol are Potent Quorum Sensing Inhibitors of Staphylococcus aureus Virulence

Romero (Rosmarinus officinalis L.): su origen, importancia y generalidades de sus metabolitos secundarios

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