Anti-biofilm activity of chitosan gels formulated with silver nanoparticles and their cytotoxic effect on human fibroblasts

Pérez-Díaz, Mario Alberto; Alvarado-Gómez, Elizabeth; Magaña-Aquino, Martín; Sánchez-Sánchez, Roberto; Velasquillo, Cristina; González, Carmen; Ganem-Rondero, Adriana; Martínez-Castañón, Gabriel Alejandro; Zavala-Alonso, Norma Verónica; Martínez-Gutiérrez, Fidel

doi:10.1016/j.msec.2015.11.036

Cited by 96 publications

(37 citation statements)

References 38 publications

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“…Fibroblasts are the principal dermal cells and are integral to the wound healing process 60. Therefore, hFFs were used in a scratch closure assay, where the hFFs supplemented with BCM failed to close properly, as Kirker et al61 observed previously.…”

Section: Discussionmentioning

confidence: 98%

Evaluation of a 2-aminoimidazole variant as adjuvant treatment for dermal bacterial infections

Draughn¹,

Allen²,

Routh

et al. 2017

DDDT

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2-Aminoimidazole (2-AI)-based compounds have been shown to efficiently disrupt biofilm formation, disperse existing biofilms, and resensitize numerous multidrug-resistant bacteria to antibiotics. Using Pseudomonas aeruginosa and Staphylococcus aureus, we provide initial pharmacological studies regarding the application of a 2-AI as a topical adjuvant for persistent dermal infections. In vitro assays indicated that the 2-AI H10 is nonbactericidal, resensitizes bacteria to antibiotics, does not harm the integument, and promotes wound healing. Furthermore, in vivo application of H10 on swine skin caused no gross abnormalities or immune reactions. Taken together, these results indicate that H10 represents a promising lead dermal adjuvant compound.

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Section: Discussionmentioning

confidence: 98%

Evaluation of a 2-aminoimidazole variant as adjuvant treatment for dermal bacterial infections

Draughn¹,

Allen²,

Routh

et al. 2017

DDDT

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“…18 The broad-spectrum antibacterial effectiveness of AgNPs is due to their small size that enhances the accumulation in the microbial membrane and thus results in disruption and loss of integrity of the microbial membrane. 8,[19][20][21] AgNPs are fabricated by different methods including chemical, physical, and green methods. 1,3,22,23 Chemical and physical methods are usually expensive, with low production rates, and are not ecofriendly due to the toxicity of the used chemical-reducing agents and capping agents.…”

Section: Introductionmentioning

confidence: 99%

“…Several authors reported the preparation of topically applied formulations intended for the delivery of AgNPs. 21,24,29,30 The present work aimed at biological synthesis of AgNPs in a simple, ecofriendly, and cost-effective manner using fungus Fusarium verticillioides ASU1 (KT587649). A comparative detailed study based on the effect of three factors on AgNPs activity was provided.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels

Mekkawy

El‐Mokhtar²,

Nafady

et al. 2017

IJN

150

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In the present study, silver nanoparticles (AgNPs) were synthesized via biological reduction of silver nitrate using extract of the fungus Fusarium verticillioides (green chemistry principle). The synthesized nanoparticles were spherical and homogenous in size. AgNPs were coated with polyethylene glycol (PEG) 6000, sodium dodecyl sulfate (SDS), and β-cyclodextrin (β-CD). The averaged diameters of AgNPs were 19.2±3.6, 13±4, 14±4.4, and 15.7±4.8 nm, for PEG-, SDS-, and β-CD-coated and uncoated AgNPs, respectively. PEG-coated AgNPs showed greater stability as indicated by a decreased sedimentation rate of particles in their water dispersions. The antibacterial activities of different AgNPs dispersions were investigated against Gram-positive bacteria (methicillin-sensitive and methicillin-resistant Staphylococcus aureus ) and Gram-negative bacteria ( Escherichia coli ) by determination of the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs). MIC and MBC values were in the range of 0.93–7.5 and 3.75–15 µg/mL, respectively, which were superior to the reported values in literature. AgNPs-loaded hydrogels were prepared from the coated-AgNPs dispersions using several gelling agents (sodium carboxymethyl cellulose [Na CMC], sodium alginate, hydroxypropylmethyl cellulose, Pluronic F-127, and chitosan). The prepared formulations were evaluated for their viscosity, spreadability, in vitro drug release, and antibacterial activity, and the combined effect of the type of surface coating and the polymers utilized to form the gel was studied. The in vivo wound-healing activity and antibacterial efficacy of Na CMC hydrogel loaded with PEG-coated AgNPs in comparison to the commercially available silver sulfadiazine cream (Dermazin ® ) were evaluated. Superior antibacterial activity and wound-healing capability, with normal skin appearance and hair growth, were demonstrated for the hydrogel formulations, as compared to the silver sulfadiazine cream. Histological examination of the treated skin was performed using light microscopy, whereas the location of AgNPs in the skin epidermal layers was visualized using transmission electron microscopy.

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“…This, together with the sustained drug release via hydrogel, can result in higher drug/active substances' concentrations at the skin site while reducing possible systemic absorption and consequent adverse effects [15].Chitosan represents one of the most studied natural polymers for the development of effective hydrogel-based wound dressings due to its numerous intrinsic biological properties. Its biocompatibility and bioadhesiveness, together with intrinsic bacteriostatic effect, make this biopolymer effective in promoting wound healing while also preventing biofilm formation [16,17]. In spite of the numerous advantages of chitosan hydrogels, the hydrophilic environment provided by chitosan hydrogel limits the incorporation of lipophilic drugs/active compounds and remains a pharmaceutical challenge.Liposomes as carriers for poorly soluble drugs/active molecules assist in overcoming this hydrogel limitation; the combination of these two delivery systems is a promising approach to achieve controlled dermal drug delivery and effective localized skin therapy [18][19][20].…”

mentioning

confidence: 99%

“…Chitosan represents one of the most studied natural polymers for the development of effective hydrogel-based wound dressings due to its numerous intrinsic biological properties. Its biocompatibility and bioadhesiveness, together with intrinsic bacteriostatic effect, make this biopolymer effective in promoting wound healing while also preventing biofilm formation [16,17]. In spite of the numerous advantages of chitosan hydrogels, the hydrophilic environment provided by chitosan hydrogel limits the incorporation of lipophilic drugs/active compounds and remains a pharmaceutical challenge.…”

mentioning

confidence: 99%

Curcumin-In-Deformable Liposomes-In-Chitosan-Hydrogel as a Novel Wound Dressing

et al. 2019

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A liposomes-in-hydrogel system as an advanced wound dressing for dermal delivery of curcumin was proposed for improved chronic wound therapy. Curcumin, a multitargeting poorly soluble active substance with known beneficial properties for improved wound healing, was incorporated in deformable liposomes to overcome its poor solubility. Chitosan hydrogel served as a vehicle providing superior wound healing properties. The novel system should assure sustained skin delivery of curcumin, and increase its retention at the skin site, utilizing both curcumin and chitosan to improve the therapy outcome. To optimize the properties of the formulation and determine the effect of the liposomal charge on the hydrogel properties, curcumin-containing deformable liposomes (DLs) with neutral (NDLs), cationic (CDLs), and anionic (ADLs) surface properties were incorporated in chitosan hydrogel. The charged DLs affected the hydrogel's hardness, cohesiveness, and adhesiveness. Importantly, the incorporation of DLs, regardless of their surface charge, in chitosan hydrogel did not decrease the system's bioadhesion to human skin. Stability testing revealed that the incorporation of CDLs in hydrogel preserved hydrogel´s bioadhesiveness to a higher degree than both NDLs and ADLs. In addition, CDLs-in-hydrogel enabled the most sustained skin penetration of curcumin. The proposed formulation should be further evaluated in a chronic wound model.Curcumin has been proposed as one of the most promising [5]. Moreover, curcumin is a molecule able to active multiple pathways, contributing to improved wound healing [6]. The antioxidant, anti-inflammatory, and anti-bacterial properties of this pleiotropic molecule need to be more utilized [7,8]. Although curcumin is a very promising molecule to be included in wound dressings, its hydrophobicity, extensive metabolism, and limited skin penetration often hamper its wider use [9]. We have recently proven that curcumin, a multitargeting active substance, exhibits superior anti-inflammatory properties when incorporated in tailored deformable liposomes [10]. In this work, we went a step further in the development of curcumin-based wound dressings and combined the beneficial properties of curcumin as an active moiety and chitosan hydrogel as a superior vehicle to form a bioactive wound dressing targeting the treatment of chronic wounds.An ideal dressing should assure an enhanced therapeutic outcome and reduction of pain accompanied with wound dressing changes [11]. Among the possible material to be used in the manufacturing of bioactive dressings, natural polymer-based wound dressings, such as hydrogels, represent promising advanced delivery systems with a low risk of toxicity and side effects due to their biodegradability and biocompatibility [12,13]. Hydrogels mimic the biochemical, biomechanical, and structural features of the extracellular matrix (ECM), serving as superior matrices for wound treatment [13,14]. Hydrogels additionally possess good bioadhesiveness that can contribute to a prolonged retention...

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Anti-biofilm activity of chitosan gels formulated with silver nanoparticles and their cytotoxic effect on human fibroblasts

Cited by 96 publications

References 38 publications

Evaluation of a 2-aminoimidazole variant as adjuvant treatment for dermal bacterial infections

Evaluation of a 2-aminoimidazole variant as adjuvant treatment for dermal bacterial infections

In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels

Curcumin-In-Deformable Liposomes-In-Chitosan-Hydrogel as a Novel Wound Dressing

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