Anti-angiogenic effect of the total flavonoids in Scutellaria barbata D. Don

Abstract

BackgroundAngiogenesis is closely related to the growth, invasion and metastasis of tumors, also considered as the key target of anticancer therapy. Scutellaria barbata D. Don (S. barbata), a traditional Chinese medicine, is being used to treat various diseases, including cancer. However, the antitumor molecular mechanism of S. barbata was still unclear. This study aimed to investigate the inhibitory effects of the total flavones in S. barbata (TF-SB) on angiogenesis.MethodsHuman umbilical vein endothelial cells (HUVECs) were treated with various concentrations of TF-SB. Cell viability was examined using the MTT assay. The scratch assay was used to detect the migration of HUVECs after treatment with TF-SB. The ability of HUVECs to form network structures in vitro was demonstrated using the tube formation assay. The chick embryo chorioallantoic membrane assay was performed to detect the in vivo anti-angiogenic effect. The expression of VEGF was measured by the enzyme-linked immunosorbent.ResultsResults showed that TF-SB inhibited the proliferation and migration of HUVECs in a dose- dependent manner. Simultaneously, TF-SB significantly suppressed HUVEC angiogenesis in vitro and in vivo. Furthermore, VEGF was downregulated in both HUVECs and MHCC97-H cells after TF-SB treatment.ConclusionTF-SB could suppress the process of angiogenesis in vitro and in vivo. TF-SB potentially suppresses angiogenesis in HUVECs by regulating VEGF. These findings suggested that TF-SB may serve as a potent anti-angiogenic agent.

Traditional Chinese medicine in the prevention and treatment of cancer and cancer metastasis

“…Similar effects were observed in vivo using a chorioallantois membrane assay, whereby treatment with TF-SB significantly decreased the number of blood vessels formed compared with the control groups. The study further established that TF-SB treatment affected the expression of and decreased the levels of VEGF, a trend that was also correlated with the treatment concentration (89). This result is consistent with that of an earlier study, which revealed an inhibitory effect of S. barbata extract on endothelial cell migration and proliferation (90).…”

Characterization and quantification of the chemical compositions of Scutellariae Barbatae herba and differentiation from its substitute by combining UHPLC–PDA–QTOF–MS/MS with UHPLC–MS/MS

“…Modern pharmacological studies have showed that S. barbata (SB) has a wide variety of medical applications due to its antioxidant, anticancer, as well as anti-angiogenic effects, etc. [2][3][4]. Phytochemistry research found that flavonoids constituents, such as scutellarin, naringenin, apigenin, luteolin and wogonin, are the main effective components of SB [5][6][7][8][9].…”

Constituents from Scutellaria barbata Inhibiting Nitric Oxide Production in LPS‐Stimulated Microglial Cells

“…[19] In addition, S. barbata also exhibited anti-tumor effects on liver cancer. [20][21] The flavonoids in S. barbata reduced [22] the invasion of hepatocarcinoma cells [23] by inhibiting the angiogenesis. The flavonoids responsible for this activity are including scutellarin, baicalin, luteolin, naringin, wogonin, and apigenin.…”