Antagonistic potential of Streptomyces cellulosae SM12 against Ganoderma sp. TB3 and Ganoderma sp. TB4

Abstract

A study was carried out to screen 25 Actinomycetes isolates that have ability to act as biocontrol agents Actinomycetes against Ganoderma sp. TB3 and Ganoderma sp. TB4. Among 25 Actinomycetes isolates, only Actinomycetes SM12 can inhibit the growth of both Ganoderma sp. TB3 (52 %) and Ganoderma sp. TB4 (60 %). Antagonistic assay using cross streak method with a longer period of delayed antagonism showed higher inhibition effect to the growth of Ganoderma sp. TB3 (76 %) and Ganoderma sp. TB4 (85 %). Identification of Actinomycetes SM12 based on 16S rRNA revealed that Actinomycetes SM12 belong to Streptomyces cellulosae. The Actinomycetes SM12 isolate produces rectinaculum-apertum spore chain and forms a colony that exhibited white to greyish color. Enzyme activity test showed that the cultures are positive in amylase, protease, lipase and cellulase production respectively.

Streptomyces sp. AC04842: Genomic Insights and Functional Expression of Its Latex Clearing Protein Genes (lcp1 and lcp2) When Cultivated With Natural and Vulcanized Rubber as the Sole Carbon Source

“…S. cellulosae (species and subspecies) have been reported to produce more than 7 useful compounds. Compounds include gabisones, carba sugars, antitumor compound (hexacyclinic acid), proteases, lipases (detergent compatible), phenolic antioxidant compounds, biocontrol compounds ( Ganoderma and Sclerotium rolfsii ), and compounds that induce plant resistance against tobacco mosaic virus ( Ogawara, 1993 ; Regina et al, 2000 ; Muro et al, 2014 ; Rani et al, 2018 ; Attia Abo-Zaid et al, 2020 ; Colobbio et al, 2021 ; Zulfa et al, 2021 ).…”

A Scientometric and Bibliometric Analysis for Actinomycetes Research - Current Status and Future Trends

“…Actinomycetes are known to be capable of reducing pathogen activities, such as inhibitory activity of mycelial growth of B. cinerea on tomatoes by 52.38% to 96.19% (Lahmyed et al, 2021); in vitro inhibition up to 85% hyphal desegregation, and antibacterial activity against soilborne-pathogens on Solanum lycopersicum and Daucus carota (Djebaili et al, 2021); development suppression of Alternaria brassicicola on cabbage with increases of ≥80% protection level compared to control (Hassan et al, 2017); reduce damping-off incidence of Pythium aphanidermatum in cucumber by up to 71% (Costa et al, 2013); and inhibit mycelial growth of Fusarium solani by up to 70% (Torres-Rodriguez et al, 2022). These pathogen suppressing activities are caused by their ability to produce several enzymes, i.e., amylase, protease, cellulase, chitinase, esterases, and lecithinase (Loliama et al, 2013;Lahmyed et al, 2021;Zulfa et al, 2021;Díaz-Díaz et al, 2022). In addition, they also produce antibiotics, antifungal substances, plant growth factors (Zulfa et al, 2021), siderophores, kaempferol, isoscutellarin, umbelliferone, and Cichoriin (Jaber & Fayyadh, 2019).…”

“…These pathogen suppressing activities are caused by their ability to produce several enzymes, i.e., amylase, protease, cellulase, chitinase, esterases, and lecithinase (Loliama et al, 2013;Lahmyed et al, 2021;Zulfa et al, 2021;Díaz-Díaz et al, 2022). In addition, they also produce antibiotics, antifungal substances, plant growth factors (Zulfa et al, 2021), siderophores, kaempferol, isoscutellarin, umbelliferone, and Cichoriin (Jaber & Fayyadh, 2019). As a biological control against plant diseases, actinomycetes may be used as a substitute for pesticides (Djebaili et al, 2021).…”

“…Several studies related to the use of Streptomyces as a biocontrol agent include: the use of Streptomyces to inhibit Ganoderma sp. (Zulfa et al, 2021), Alternaria brassicicola on cabbage seedlings (Hassan et al, 2017), charcoal rot disease on cowpea and mungbean (Jaber & Fayyadh, 2019), damping-off disease in cucumber (Costa et al, 2013), Macrophomina phaseolina and Rhizoctonia solani on Phaseolus vulgaris (Díaz-Díaz et al, 2022), Fusarium solani from tomato (Torres-Rodriguez et al, 2022), etc. In contrast, there is currently relatively little research on actinomycetes from Indonesia.…”