Antagonistic effect of Streptomyces spp. on spore germination and mycelial growth of Fusarium oxysporum f.sp. lycopersici

Mujoko, Tri; Sastrahidayat, Ika Rochdjatun; Hadiastono, Tutung; Djauhari, Syamsuddin

doi:10.12692/ijb/5.9.414-422

Cited by 4 publications

(1 citation statement)

References 10 publications

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“…Spore germination assays were performed as described by Mujoko et al (2014) with minor modifications. The B. velezensis F21 culture in agar media was fragmented by a cork borer, and the top layer was sliced.…”

Section: Methodsmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals the Biocontrol Mechanism of Bacillus velezensis F21 Against Fusarium Wilt on Watermelon

Jiang

Yao

Mi³

et al. 2019

Front. Microbiol.

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The watermelon ( Citrullus lanatus ) is one of the most important horticultural crops for fruit production worldwide. However, the production of watermelon is seriously restricted by one kind of soilborne disease, Fusarium wilt, which is caused by Fusarium oxysporum f. sp. niveum (Fon). In this study, we identified an efficient PGPR strain B. velezensis F21, which could be used in watermelon production for Fon control. The results of biocontrol mechanisms showed that B. velezensis F21 could suppress the growth and spore germination of Fon in vitro . Moreover, B. velezensis F21 could also enhance plant basal immunity to Fon by increasing the expression of plant defense related genes and activities of some defense enzymes, such as CAT, POD, and SOD. To elucidate the detailed mechanisms regulating B. velezensis F21 biocontrol of Fusarium wilt in watermelon, a comparative transcriptome analysis using watermelon plant roots treated with B. velezensis F21 or sterile water alone and in combination with Fon inoculation was conducted. The transcriptome sequencing results revealed almost one thousand ripening-related differentially expressed genes (DEGs) in the process of B. velezensis F21 triggering ISR (induced systemic resistance) to Fon. In addition, the Gene Ontology (GO) classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment indicated that numerous of transcription factors (TFs) and plant disease resistance genes were activated and validated by using quantitative real-time PCR (qRT-PCR), which showed significant differences in expression levels in the roots of watermelon with different treatments. In addition, genes involved in the MAPK signaling pathway and phytohormone signaling pathway were analyzed, and the results indicated that B. velezensis F21 could enhance plant disease resistance to Fon through the above related genes and phytohormone signal factors. Taken together, this study substantially expands transcriptome data resources and suggests a molecular framework for B. velezensis F21 inducing systemic resistance to Fon in watermelon. In addition, it also provides an effective strategy for the control of Fusarium wilt in watermelon.

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Section: Methodsmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals the Biocontrol Mechanism of Bacillus velezensis F21 Against Fusarium Wilt on Watermelon

Jiang

Yao

Mi³

et al. 2019

Front. Microbiol.

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Tacrolimus analogue produced by Bacillus amyloliquefaciens HSSN09 suppresses watermelon Fusarium wilt by antagonizing FON

Yang,

Wang

et al. 2024

Biological Control

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Streptomyces spp. as biocontrol agents against Fusarium species.

Bubici¹

2018

CABI Reviews

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Streptomycetes are the largest taxon of antibiotic producers in the microbial world. Nevertheless, they are less studied than other biocontrol agents against Fusarium diseases and, perhaps, plant diseases in general. Plant diseases incited by Fusarium species are notably challenging. Four species complexes in the genus are pathogenic: Fusarium fujikuroi, F. graminearum, F. solani and F. oxysporum. Being a vascular pathogen, F. oxysporum is particularly difficult to control by using microbial antagonists. Most research has remained at early experimental stages, and only few Streptomyces spp. strains have been assayed under diverse conditions. Few commercial products consisting of Streptomyces spp. have often provided fluctuating results across trials. Five biocontrol trials conducted in the field using streptomycetes report reductions of diverse Fusarium wilts by 0-55%, with one case (cucumber) of yield increase by 1.3-fold. Among 38 articles dealing with pot-experiments, 16 report a disease control level above 50%, seven of which with a level above 70%. The chitinolytic activity of Streptomyces spp. strains plays an important role in the biocontrol of Fusarium diseases, and the plant growth promotion trait is a cherished outcome. More attention is being paid to strains endophytic, producing volatile organic compounds, or to the identification of antibiotics and metabolites responsible for the biocontrol. Bioformulation is a critical point in the use of biocontrol agents, but it has been still poorly considered in the experiments. The biocontrol efficacy of well-designed streptomycete consortia or the so-called microbial synthetic communities, possibly in proper bioformulations, and the role of streptomycetes in the reduction of mycotoxins in the grains are aspects that would merit further investigation in the future.

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Antagonistic effect of Streptomyces spp. on spore germination and mycelial growth of Fusarium oxysporum f.sp. lycopersici

Cited by 4 publications

References 10 publications

Comparative Transcriptome Analysis Reveals the Biocontrol Mechanism of Bacillus velezensis F21 Against Fusarium Wilt on Watermelon

Comparative Transcriptome Analysis Reveals the Biocontrol Mechanism of Bacillus velezensis F21 Against Fusarium Wilt on Watermelon

Tacrolimus analogue produced by Bacillus amyloliquefaciens HSSN09 suppresses watermelon Fusarium wilt by antagonizing FON

Streptomyces spp. as biocontrol agents against Fusarium species.

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