Anoxybacillus pushchinensis gen. nov., sp. nov., a novel anaerobic, alkaliphilic, moderately thermophilic bacterium from manure, and description of Anoxybacillus flavitherms comb. nov.

Pikuta, E. N.; Lysenko, A.V.; Chuvil’skaya, N. A.; Mendrock, Ulrike; Hippe, Hans; Suzina, N. E.; Nikitin, Dmitriy; Осипов, Г. А.; Laurinavichius, Kestas

doi:10.1099/00207713-50-6-2109

Cited by 186 publications

(138 citation statements)

References 25 publications

Supporting

Mentioning

136

Contrasting

Unclassified

Order By: Relevance

“…The present paper describes the isolation, morphological, physiological and biochemical profiles and 16S rRNA sequence of this strain and the results of DNA-DNA hybridization with close relatives and proposes that it represents a novel species of the genus Anoxybacillus (Pikuta et al, 2000), Anoxybacillus gonensis sp. nov.…”

mentioning

confidence: 99%

Anoxybacillus gonensis sp. nov., a moderately thermophilic, xylose-utilizing, endospore-forming bacterium

Beldüz

Dülger

Demirbağ

2003

International Journal of Systematic and Evolutionary Microbiology

107

View full text Add to dashboard Cite

Seven closely related xylanolytic, thermophilic bacilli were isolated from mud and water samples from the Gonen and Diyadin hot springs, respectively located in the Turkish provinces of Balikesir and Agri. On the basis of morphology and biochemical characteristics, one of the isolates, designated strain G2 T , was studied further. Strain G2 T is a xylanolytic, sporulating, Grampositive, rod-shaped bacterium. The isolate is a thermophilic (optimum temperature for growth, 55-60˚C), facultative anaerobe that grows on a wide range of carbon sources, including glucose, starch, xylose and mannitol. It expressed a high level of xylose isomerase activity on xylose and also on glucose. 16S rRNA gene sequence analysis showed that this isolate resembled Anoxybacillus flavithermus DSM 2641 T (>97 % similarity), but 16S-23S rDNA internally transcribed spacer polymorphism PCR showed variation between DSM 2641 T and isolate G2 T . However, it is also known that analysis of 16S rRNA gene sequences may be insufficient to distinguish between some species. In DNA-DNA hybridization, thermophilic isolate G2 T showed relatedness of 53?4 % to A. flavithermus and about 45?0 % to Anoxybacillus pushchinoensis, indicating that it is distinct at the species level. On the basis of the evidence presented, it is proposed that strain G2 T (=NCIMB 13933 T =NCCB 100040 T ) be designated as the type strain of Anoxybacillus gonensis sp. nov.It is now over a century since thermophiles were first reported (Miquel, 1888). Over the years, a number of sporeforming thermophiles have been reported, mainly in the genera Bacillus and Clostridium (Guagliardi et al., 1996).In this study, we isolated some thermophilic bacilli from the Gonen and Diyadin hot springs, respectively located in the Turkish provinces of Balikesir and Agri. On the basis of preliminary experiments, a representative strain appeared to differ from other thermophilic bacilli with respect to the utilization of xylose; it was therefore characterized further. Xylose isomerase is an intracellular enzyme that catalyses the conversion of D-xylose to D-xylulose. Its practical significance stems from its ability to isomerize D-glucose to D-fructose. Therefore, this enzyme is often referred to as glucose isomerase and is widely used in industry for the production of high-fructose corn syrup.The present paper describes the isolation, morphological, physiological and biochemical profiles and 16S rRNA sequence of this strain and the results of DNA-DNA hybridization with close relatives and proposes that it represents a novel species of the genus Anoxybacillus (Pikuta et al., 2000), Anoxybacillus gonensis sp. nov. Isolation of strainsSeven Gram-positive rods were isolated from mud and water samples from the Gonen and Diyadin hot springs. The water temperature of these hot springs is around 70 uC. After collection, mud and water samples were used immediately for enrichment in nutrient broth at 60-70 uC. Oneday-old enrichment cultures were repeatedly subcultured in 10 ml nutrient broth and streaked on ...

show abstract

mentioning

confidence: 99%

Anoxybacillus gonensis sp. nov., a moderately thermophilic, xylose-utilizing, endospore-forming bacterium

Beldüz

Dülger

Demirbağ

2003

International Journal of Systematic and Evolutionary Microbiology

107

View full text Add to dashboard Cite

show abstract

“…The presence of adaptive genes is sufficient for the cells to live in an alkaline environment with the presence of organic nitrogen and carbohydrates and to overcome the threats from UV radiation. In addition, for Anoxybacillus to survive under extreme conditions, we think that genetic exchange, especially uptake of genetic material via HGT, is important (Pikuta et al, 2000) and (Saw et al, 2008)and original.…”

Section: Effect Of Phmentioning

confidence: 99%

Study on Degradation of Nylon 6 by thermophilic bacteria Anoxybacillus rupiensis Ir3 (JQ912241)

2016

Int. J. Adv. Res. Biol. Sci.

View full text Add to dashboard Cite

Polyamide-6 (also known as nylon-6) is one of the biodegradable-resistant synthetic polymers used in the manufacturing of commodity plastic materials. The environmental effects of the persistence of this material in landfill pose a global problem of disposal system. Knowledge of the microbial pattern of interaction with this plastic will provide the biological resources and scientific basis for the development of sustainable disposal and treatment method. Anoxybacillus rupiensis Ir3 (JQ912241) is a novel thermophilic bacterium, it was isolated from hydrocarbon contaminated soil in Iraq and showed good ability to utilize aromatic compounds, It is represented a new carbazole-degrading bacterium. In an attempt to investigate the ability of this bacterium to degrade nylon6, the strain Ir3 was grown on the nylon6 separately as a sole source of carbon and nitrogen. Results showed that strain Ir3 was able to degrade this compound. Optimum conditions for degradation of nylon6 were investigated. It was found that these conditions are growing this bacterium in chemical define media CDM containing (0.5%) nylon6, and incubated with shaking (180rpm) at 65°C for 7 days, and to confirm the ability to degrade nylon6, analytical experiments HPLC (High Performance Liquid Chromatography) and FTIR (Fourier Transmittance Infrared Spectroscopy) were used. The 6-aminohexanoic acid as intermediate products in the culture medium was mentioned by using HPLC, while biodegradation of the nylon6 was monitored by using FTIR.

show abstract

“…The name given to the genus suggests that species assigned to it thrive under anoxic conditions, and, indeed, the first species classified as members of the genus Anoxybacillus were aerotolerant anaerobes (Pikuta et al, 2000). Nevertheless, at the time of writing, from the 22 species of the genus Anoxybacillus, 16 are facultative anaerobes (Atanassova et al, 2008;Belduz et al, 2003;Cihan et al, 2011Cihan et al, , 2014Dai et al, 2011;De Clerck et al, 2004;Dulger et al, 2004;Kevbrin et al, 2005;Namsaraev et al, 2010;Poli et al, 2006;Schaffer et al, 2004;Yumoto et al, 2004;Zhang et al, 2011), five are strict aerobes (Coorevits et al, 2012;Derekova et al, 2007;Inan et al, 2013;Poli et al, 2009;Zhang et al, 2013) and one is an aerotolerant anaerobe (Pikuta et al, 2000). Species that belong to the genus Anoxybacillus share common characteristics, mainly concerning their major fatty acids (iso-C 17 : 0 and iso-C 15 : 0 ), the rod shape of the vegetative cells and the terminal/subterminal position of their endospores.…”

mentioning

confidence: 99%

Anoxybacillus geothermalis sp. nov., a facultatively anaerobic, endospore-forming bacterium isolated from mineral deposits in a geothermal station

Filippidou

Jaussi

Junier

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

A novel endospore-forming bacterium designated strain GSsed3 T was isolated from deposits clogging aboveground filters from the geothermal power platform of Groß Schönebeck in northern Germany. The novel isolate was Gram-staining-positive, facultatively anaerobic, catalase-positive and oxidase-positive. Optimum growth occurred at 60 C, 0.5 % (w/v) NaCl and pH 7-8. Analysis of the 16S rRNA gene sequence similarity indicated that strain GSsed3 T belonged to the genus Anoxybacillus, and showed 99.8 % sequence similarity to Anoxybacillus rupiensis R270 T , 98.2 % similarity to Anoxybacillus tepidamans GS5-97 T , 97.9 % similarity to Anoxybacillus voinovskiensis TH13 T , 97.7 % similarity to Anoxybacillus caldiproteolyticus DSM 15730 T and 97.6 % similarity to Anoxybacillus amylolyticus MR3C T . DNA-DNA hybridization (DDH) indicated only 16 % relatedness to Anoxybacillus rupiensis DSM 17127 T . Furthermore, DDH estimation based on genomes analysis indicated only 19.9 % overall nucleotide similarity to Anoxybacillus amylolyticus DSM 15939 T . The major respiratory menaquinone was MK-8. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unknown phosphoglycolipid and one unknown phospholipid. The predominant cellular fatty acids were iso-C 15 : 0 , iso-C 17 : 0 , C 16 : 0 , iso-C 16 : 0 and anteiso-C 17 : 0 . The peptidoglycan type was A1g meso-Dpm-direct. The genomic DNA G+C content of the strain was 46.9 mol%. The phenotypic, genotypic and chemotaxonomic characterization indicated that strain GSsed3 T differs from related species of the genus. Therefore, strain GSsed3 T is considered to be a representative of a novel species of the genus Anoxybacillus, for which the name Anoxybacillus geothermalis sp. nov. is proposed. The type strain of Anoxybacillus geothermalis is GSsed3 T (=CCOS808 T =ATCC BAA2555 T ).

show abstract

Anoxybacillus pushchinensis gen. nov., sp. nov., a novel anaerobic, alkaliphilic, moderately thermophilic bacterium from manure, and description of Anoxybacillus flavitherms comb. nov.

Cited by 186 publications

References 25 publications

Anoxybacillus gonensis sp. nov., a moderately thermophilic, xylose-utilizing, endospore-forming bacterium

Anoxybacillus gonensis sp. nov., a moderately thermophilic, xylose-utilizing, endospore-forming bacterium

Study on Degradation of Nylon 6 by thermophilic bacteria Anoxybacillus rupiensis Ir3 (JQ912241)

Anoxybacillus geothermalis sp. nov., a facultatively anaerobic, endospore-forming bacterium isolated from mineral deposits in a geothermal station

Contact Info

Product

Resources

About