Study on Degradation of Nylon 6 by thermophilic bacteria Anoxybacillus rupiensis Ir3 (JQ912241)

Abstract

Polyamide-6 (also known as nylon-6) is one of the biodegradable-resistant synthetic polymers used in the manufacturing of commodity plastic materials. The environmental effects of the persistence of this material in landfill pose a global problem of disposal system. Knowledge of the microbial pattern of interaction with this plastic will provide the biological resources and scientific basis for the development of sustainable disposal and treatment method. Anoxybacillus rupiensis Ir3 (JQ912241) is a novel thermophilic bacterium, it was isolated from hydrocarbon contaminated soil in Iraq and showed good ability to utilize aromatic compounds, It is represented a new carbazole-degrading bacterium. In an attempt to investigate the ability of this bacterium to degrade nylon6, the strain Ir3 was grown on the nylon6 separately as a sole source of carbon and nitrogen. Results showed that strain Ir3 was able to degrade this compound. Optimum conditions for degradation of nylon6 were investigated. It was found that these conditions are growing this bacterium in chemical define media CDM containing (0.5%) nylon6, and incubated with shaking (180rpm) at 65°C for 7 days, and to confirm the ability to degrade nylon6, analytical experiments HPLC (High Performance Liquid Chromatography) and FTIR (Fourier Transmittance Infrared Spectroscopy) were used. The 6-aminohexanoic acid as intermediate products in the culture medium was mentioned by using HPLC, while biodegradation of the nylon6 was monitored by using FTIR.

A Thermostable Lipase Isolated from Brevibacillus thermoruber Strain 7 Degrades Ɛ-Polycaprolactone

“…Extremophilic microorganisms and relevant extremophilic enzymes appear to be a promising solution to the plastic accumulation problem due to the changes that occur in polymer properties at extreme values of temperature, pH, and salinity. Among the few reports available for thermophilic plastic degraders are polyethylene degradation by Brevibaccillus borstelensis [ 11 ], polyethylene terephthalate by Clostridium thermocellum [ 12 ], and nylon degradation by Anoxybacillus rupiensis and Geobacillus thermocatenulatus [ 13 , 14 ]. Thermophilic enzymes able to degrade PCL could be especially useful in plastic degradation due to its low melting point of around 60 °C.…”