Anomalously high cooperativity of oligodeoxycytidylic acid for luminescence resonance energy transfer to lanthanide ions

Abstract

The luminescence of terbium(III) and europium(III) through luminescence resonance energy transfer from mononucleotides and oligodeoxynucleotides is examined. Among mononucleotides, dGMP gives the strongest luminescence of terbium(III), while dTMP and dCMP yield a luminescence intensity of europium(III) that is larger than the other two cases. In the homodeoxydecamers, decadeoxycytidylic acid (dC10) produces the highest intensity for both metals. The anomalously large cooperativity of dC10 is explained by the easiness of deformation of the helical structure to bind lanthanide ions, and a circular dichroism study supports this explanation.

Interactions of the human telomeric DNA with terbium–amino acid complexes

“…2A, in comparison with I-motif DNA, G-quadruplex DNA can significantly increase the fluorescence of the complex. This is consistent with previous reports that the energy transfer is more efficient for guanine bases to terbium [21,22]. Control experiments for TbCl 3 binding to G-quadruplex DNA showed that 32-fold fluorescence enhancement was observed.…”