Annealing-modulated nanoscintillators for nonconventional X-ray activation of comprehensive photodynamic effects in deep cancer theranostics

Chuang, Yao-Chen; Chu, Chia-Hui; Cheng, Shih-Hsun; Liao, Lun-De; Chu, Tsung-Sheng; Chen, Nai-Tzu; Paldino, Arthur; Hsia, Yu; Chen, Chin-Tu; Lo, Leu‐Wei

doi:10.7150/thno.41752

Cited by 23 publications

(35 citation statements)

References 64 publications

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“…Alternatively, photosensitizer-independent X-ray photodynamic therapy using Y 2 O 3 based NPs could be of interest for precision therapeutic treatments of deep-seated tumors [ 10 ]. Our experiments of irradiation with superficial X-ray sources used in radiotherapy for skin cancer were found to induce cellular damage via ROS generation.…”

Section: Discussionmentioning

confidence: 99%

“…In next experiments, we found that the colony-forming assay rather supports the synergic effect of NPs and radiation with eventual impact on radiotherapy enhancement. By adding the NPs to cells before irradiation, one may clearly observe decreasing the number of proliferating colonies-generating cells, most probably due to cell apoptosis [ 10 ]. Indeed, cell apoptosis may be induced by either mitochondrial injury we have observed at 6 Gy irradiation or DNA damage.…”

Section: Discussionmentioning

confidence: 99%

“…More recently, Kertzscher et al analyzed a mixture of Y 2 O 3 :Eu and Yttrium orthovanadate doped with Europium (YVO 4 :Eu) as scintillating detector aimed at in vivo dosimetry for 192 Ir brachytherapy [ 9 ]. Additionally, Chuang et al showed that Y 2 O 3 :Eu covered by silica shell (Y 2 O 3 : Eu@SiO 2 ) NPs act as independent photosensitizers for X-ray induced photodynamic therapy by generating an increased amount of reactive oxygen species (ROS) under X-ray irradiation that lead to cancer cell death [ 10 ]. This was the confirmation that Y 2 O 3 -based NPs may directly induce X-ray photodynamic effects without the presence of conventional photosensitizers.…”

Section: Introductionmentioning

confidence: 99%

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Y2O3 Nanoparticles and X-ray Radiation-Induced Effects in Melanoma Cells

et al. 2021

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The innovative strategy of using nanoparticles in radiotherapy has become an exciting topic due to the possibility of simultaneously improving local efficiency of radiation in tumors and real-time monitoring of the delivered doses. Yttrium oxide (Y2O3) nanoparticles (NPs) are used in material science to prepare phosphors for various applications including X-ray induced photodynamic therapy and in situ nano-dosimetry, but few available reports only addressed the effect induced in cells by combined exposure to different doses of superficial X-ray radiation and nanoparticles. Herein, we analyzed changes induced in melanoma cells by exposure to different doses of X-ray radiation and various concentrations of Y2O3 NPs. By evaluation of cell mitochondrial activity and production of intracellular reactive oxygen species (ROS), we estimated that 2, 4, and 6 Gy X-ray radiation doses are visibly altering the cells by inducing ROS production with increasing the dose while at 6 Gy the mitochondrial activity is also affected. Separately, high-concentrated solutions of 25, 50, and 100 µg/mL Y2O3 NPs were also found to affect the cells by inducing ROS production with the increase of concentration. Additionally, the colony-forming units assay evidenced a rather synergic effect of NPs and radiation. By adding the NPs to cells before irradiation, a decrease of the number of proliferating cell colonies was observed with increase of X-ray dose. DNA damage was evidenced by quantifying the γ-H2AX foci for cells treated with Y2O3 NPs and exposed to superficial X-ray radiation. Proteomic profile confirmed that a combined effect of 50 µg/mL Y2O3 NPs and 6 Gy X-ray dose induced mitochondria alterations and DNA changes in melanoma cells.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Y2O3 Nanoparticles and X-ray Radiation-Induced Effects in Melanoma Cells

et al. 2021

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“…The addition of a PS without traditional effects reduces the oxygen dependence of the complex, which can subsequently produce a process similar to type I PDT under ionizing radiation. Chuang YC [191] introduced the annealing process to achieve PS-free PDT. The yttrium oxide nanoscintillation complex coated with a silica shell (Y 2 O 3 :Eu@SiO 2 ) was subjected to X-ray irradiation and subsequent annealing, and then, photodynamic effects were promoted to mediate tumor cell damage in conjunction with radiotherapy.…”

Section: Radiotherapymentioning

confidence: 99%

Progress in Nanocarriers Codelivery System to Enhance the Anticancer Effect of Photodynamic Therapy

2021

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Photodynamic therapy (PDT) is a promising anticancer noninvasive method and has great potential for clinical applications. Unfortunately, PDT still has many limitations, such as metastatic tumor at unknown sites, inadequate light delivery and a lack of sufficient oxygen. Recent studies have demonstrated that photodynamic therapy in combination with other therapies can enhance anticancer effects. The development of new nanomaterials provides a platform for the codelivery of two or more therapeutic drugs, which is a promising cancer treatment method. The use of multifunctional nanocarriers for the codelivery of two or more drugs can improve physical and chemical properties, increase tumor site aggregation, and enhance the antitumor effect through synergistic actions, which is worthy of further study. This review focuses on the latest research progress on the synergistic enhancement of PDT by simultaneous multidrug administration using codelivery nanocarriers. We introduce the design of codelivery nanocarriers and discuss the mechanism of PDT combined with other antitumor methods. The combination of PDT and chemotherapy, gene therapy, immunotherapy, photothermal therapy, hyperthermia, radiotherapy, sonodynamic therapy and even multidrug therapy are discussed to provide a comprehensive understanding.

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“…Recently, we published several studies showing that functional PAM (fPAM) is an ideal tool for in vivo evaluation of the changes in functional cerebral blood volume (CBV) and hemoglobin oxygen saturation (SO 2 ) in normal rat brains [16][17][18] or in disease models [19][20][21]. Additionally, PAM has been extended to theranostic applications [22], such as treatment intervention [23], chemotherapy [24], and imaging-guided photothermal therapy [25,26]. Researchers have also explored PA imaging agents as contrast enhancement agents [27,28] or drug carriers [25,29], where drug release is triggered by the heat generated by the agent upon laser irradiation.…”

Section: Introductionmentioning

confidence: 99%

An Adjustable Dark-Field Acoustic-Resolution Photoacoustic Imaging System with Fiber Bundle-Based Illumination

et al. 2021

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Photoacoustic (PA) imaging has become one of the major imaging methods because of its ability to record structural information and its high spatial resolution in biological tissues. Current commercialized PA imaging instruments are limited to varying degrees by their bulky size (i.e., the laser or scanning stage) or their use of complex optical components for light delivery. Here, we present a robust acoustic-resolution PA imaging system that consists of four adjustable optical fibers placed 90° apart around a 50 MHz high-frequency ultrasound (US) transducer. In the compact design concept of the PA probe, the relative illumination parameters (i.e., angles and fiber size) can be adjusted to fit different imaging applications in a single setting. Moreover, this design concept involves a user interface built in MATLAB. We first assessed the performance of our imaging system using in vitro phantom experiments. We further demonstrated the in vivo performance of the developed system in imaging (1) rat ear vasculature, (2) real-time cortical hemodynamic changes in the superior sagittal sinus (SSS) during left-forepaw electrical stimulation, and (3) real-time cerebral indocyanine green (ICG) dynamics in rats. Collectively, this alignment-free design concept of a compact PA probe without bulky optical lens systems is intended to satisfy the diverse needs in preclinical PA imaging studies.

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Annealing-modulated nanoscintillators for nonconventional X-ray activation of comprehensive photodynamic effects in deep cancer theranostics

Cited by 23 publications

References 64 publications

Y2O3 Nanoparticles and X-ray Radiation-Induced Effects in Melanoma Cells

Y2O3 Nanoparticles and X-ray Radiation-Induced Effects in Melanoma Cells

Progress in Nanocarriers Codelivery System to Enhance the Anticancer Effect of Photodynamic Therapy

An Adjustable Dark-Field Acoustic-Resolution Photoacoustic Imaging System with Fiber Bundle-Based Illumination

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