Angiotensin I-converting enzyme inhibitory activity and antioxidant capacity of bioactive peptides derived from enzymatic hydrolysis of buffalo milk proteins

Abdel-Hamid, Mahmoud; Otte, Jeanette; Gobba, Cristian De; Osman, Ali; Hamad, Essam M.

doi:10.1016/j.idairyj.2016.11.006

Cited by 153 publications

(88 citation statements)

References 37 publications

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“…Free radical scavenging is one of the main antioxidation mechanisms inhibiting the chain reaction of lipid peroxidation (Vuda et al, 2012). In the current study, BSMRH admisntration significantly reduced the lipid peroxidation by decreasing the MDA levels, confirming the free radical scavenging activity of BSMRH in-vivo conditions as reported in our prvious study (Abdel-Hamid et al, 2017). GSH is a critical biomarker for tissue susceptibility to oxidative damage.…”

Section: Effect Of Cpre On Antioxidant Biomarkerssupporting

confidence: 92%

Hepatoprotective Effects of Crude Phenolic-rich Extract from Oyster Mushroom (Pleurotus ostreatus)

Osman

Toliba

2019

Egypt. J. Food Sci.

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I N this study the hepatoprotective activity of the crude phenolic rich extract (CPRE) isolated from oyster mushrooms on carbon tetrachloride (CCl 4)-induced oxidative stress was investigated in albino rats. The hepatoprotective activity was examined through various biochemical parameters. Administration of CCl 4 for 28 days exhibited a significant increase (P<0.05) in serum markers of liver damage, i.e., alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase, urea, creatinine, total lipids and triglycerides. Whereas, the serum markers were significantly decreased (P<0.05) by CPRE administration. CCl 4 exposure significantly decreased (P<0.05) the hepatic antioxidant enzyme activities such as superoxide dismutase and glutathione peroxidase. Contrarily, the CPRE treatments showed significant increases (P<0.05) in these hepatic activities. The results obtained from this study clearly validated the potential antioxidant activity of CPRE isolated from oyster mushrooms against several oxidation systems in-vivo, which contributed to its hepatoprotective effects in CCl 4-induced liver injury in male albino rats. Finally, oyster mushrooms could be added as an additional nutrient to food products as it constitutes a new potential source of natural antioxidant and antibacterial agents.

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Section: Effect Of Cpre On Antioxidant Biomarkerssupporting

confidence: 92%

Hepatoprotective Effects of Crude Phenolic-rich Extract from Oyster Mushroom (Pleurotus ostreatus)

Osman

Toliba

2019

Egypt. J. Food Sci.

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“…According to Rajapakse et al (2005) and Xiong (2010) the presence of cysteine, methionine, tyrosine, phenylalanine, tryptophan and proline in BP is associated with their AOX as scavenging free radicals; meanwhile the presence of lysine, arginine, glutamine, aspartic acid, threonine and phosphorylated serine in BP are associated with their AOX as metal quelators. Bertucci et al (2015) reported a high AOX by ABTS assay in whey protein hydrolysates, and similar data by Abdel-Hamid et al (2017) were found in BP derived from enzymatic hydrolysis of buffalo milk proteins. Kumar et al (2016) mentioned that the AOX (ABTS, DPPH and FRAP assays) of camel milk casein was significantly high in protein hydrolysates and their peptide fractions.…”

Section: Antioxidant Capacity Of Egg Milk and Soy Protein Hydrolysatsupporting

confidence: 70%

Antioxidant capacity of egg, milk and soy protein hydrolysates and biopeptides produced by Bromelia pinguin and Bromelia karatas-derived proteases

et al. 2018

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Given the lack of knowledge in the generation and biological activity of biopeptides (BP) produced by Bromelia pinguin and Bromelia karatas-derived proteases, the objective of this work was to evaluate the antioxidant capacity (AOX) of protein hydrolysates and peptide fractions from hydrolysis of egg, milk and soy proteins with enzymes extracted from Bromelia pinguin and Bromelia karatas fruits. The degree of hydrolysis (DH) of the mentioned proteins was measured. The hydrolysates were sequentially fractionated by ultrafiltration using different membrane pore sizes (≤30, ≤10, ≤5 and ≤1 kDa) to obtain biopeptide fractions (BPF). The antioxidant capacity (AOX) of protein hydrolysates and BPF were evaluated. AOX was performed using 2,2´-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) assays. after the enzymatic action of B. pinguin and B. karatas proteases, the highest DH values for egg, milk and soy protein hydrolysates ranged from 50-61%, 55-68% and 73-81%, respectively. The AOX was higher for soy hydrolysates than for egg and milk hydrolysates. Additionally, the highest AOX from ABTS and FRAP assays was observed for BPF ≤30 kDa, PF ≤5 kDa and ≤1 kDa, respectively. These results highlight the potential of B. pinguin and B. karatas proteases to hydrolyze food proteins and release bioactive peptides. Moreover, it has been shown that the hydrolysis products are a mixture of BP of different molecular weights, which could present AOX through different mechanisms.

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“…Para la extracción de proteasas de cada especie vegetal, se siguió la metodología descrita por Meza-Espinoza et al (2017). El extracto crudo enzimático (CE) de B. karatas se obtuvo con una solución amortiguadora de fosfato-cisteína (solución de fosfato 0.1 M, EDTA 5 mM y cisteína 5 mM ajustada a pH 6.1); mientras que el CE de B. pinguin se obtuvo con una solución amortiguadora de sulfuro de sodio (Na 2 S 2 mM y H 2 SO 4 0.2 mM, ajustado a pH 6.1).…”

Section: Extracción De Proteasasunclassified

Evaluación de la capacidad de inhibición de hemólisis oxidativa y actividad antimicrobiana de fracciones peptídicas obtenidas de la hidrólisis de proteínas de huevo, leche y soya usando proteasas extraídas de Bromelia pinguin y Bromelia karatas

et al. 2018

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Los hidrolizados proteínicos son una fuente de péptidos bioactivos (PB) y estos compuestos pueden ejercer un papel importante en la salud humana debido a sus diferentes acividades biológicas. El uso de proteasas de origen vegetal es una alternativa potencial para producir PB. El objetivo de este trabajo fue evaluar la capacidad de inhibición de la hemólisis oxidativa (IHO) y actividad antimicrobiana de hidrolizados y fracciones peptídicas obtenidas de la hidrólisis de proteínas de huevo (ovoalbúmina), leche y soya, con proteasas extraídas de frutos de Bromelia pinguin y Bromelia karatas. En general, todos los hidrolizados y fracciones peptídicas presentaron una alta IHO, siendo la fracción peptídica de ≤1 kDa, obtenida de la hidrólisis de ovoalbúmina con proteasas de B. karatas, la que presentó mayor actividad (98.19%). En contraste, ninguno de los hidrolizados registró actividad antimicrobiana, mientras que las fracciones peptídicas (≤5, ≤10 y ≤30 kDa), obtenidas de la hidrólisis de leche con proteasas de B. pinguin, mostraron la mayor actividad antimicrobina (22.26-23.79% de inhibición), contra Listeria innocua. Los resultados resaltan el potencial de las proteasas de B. pinguin y B. karatas para hidrolizar proteínas de diferentes alimentos y generar hidrolizados y PB con alta actividad antioxidante.

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Angiotensin I-converting enzyme inhibitory activity and antioxidant capacity of bioactive peptides derived from enzymatic hydrolysis of buffalo milk proteins

Cited by 153 publications

References 37 publications

Hepatoprotective Effects of Crude Phenolic-rich Extract from Oyster Mushroom (Pleurotus ostreatus)

Hepatoprotective Effects of Crude Phenolic-rich Extract from Oyster Mushroom (Pleurotus ostreatus)

Antioxidant capacity of egg, milk and soy protein hydrolysates and biopeptides produced by Bromelia pinguin and Bromelia karatas-derived proteases

Evaluación de la capacidad de inhibición de hemólisis oxidativa y actividad antimicrobiana de fracciones peptídicas obtenidas de la hidrólisis de proteínas de huevo, leche y soya usando proteasas extraídas de Bromelia pinguin y Bromelia karatas

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