Anandamide amidohydrolase activity, released in the medium by <i>Tetrahymena pyriformis</i>. Identification and partial characterization

Karava, Vivi; Fasia, Lambrini; Siafaka-Kapadai, Athanassia

doi:10.1016/s0014-5793(01)03095-2

Cited by 15 publications

(14 citation statements)

References 45 publications

(68 reference statements)

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“…The literature supports this concept. FAAH-like amidohydrolases have been extracted from Ci (Matias et al, 2005), the leech Hirudo medicinalis (Matias et al, 2001), Tetrahymena pyriformis, a species closely related to Tt, (Karava et al, 2001), and even Hydra vulgaris (De Petrocellis et al, 1999). Endocannabinoids have been extracted from Sc (Merkel et al, 2005), consistent with the presence of an FAAH ortholog in the Sc genome.…”

Section: Faahmentioning

confidence: 83%

Evolutionary origins of the endocannabinoid system

McPartland¹,

Matias

Marzo

et al. 2006

Gene

157

117

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Section: Faahmentioning

confidence: 83%

Evolutionary origins of the endocannabinoid system

McPartland¹,

Matias

Marzo

et al. 2006

Gene

157

117

View full text Add to dashboard Cite

“…In order to further explore the mechanism by which anandamide stimulates the vanilloid receptor, experiments were performed in the absence of calcium. Under such conditions, anandamide would still be subject to metabolism by FAAH (Hillard et al ., 1995; Karava et al ., 2001). Whilst depolarization to anandamide was unaffected by calcium removal, lipoxygenase inhibition failed to inhibit this response suggesting that anandamide directly activated native vanilloid receptor under these conditions.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the anandamide induced depolarization of guinea‐pig isolated vagus nerve

Kagaya

Lamb

Robbins

et al. 2002

British J Pharmacology

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1 There is considerable interest in elucidating potential endogenously derived agonists of the vanilloid receptor and the role of anandamide in this regard has received considerable attention. In the present study, we have used an electrophysiological technique to investigate the mechanism of activation of vanilloid receptors in an isolated vagal preparation. 2 Both capsaicin and anandamide depolarized de-sheathed whole vagal nerve preparations that was antagonized by the VR1 antagonist, capsazepine (P50.05) whilst this response was unaltered by the cannabinoid (CB1) selective antagonist SR141716A or the CB2 selective antagonist, SR144528, thereby ruling out a role for cannabinoid receptors in this response. 3 The PKC activator, phorbol-12-myristate-13-acetate (PMA) augmented depolarization to both anandamide and capsaicin and this response was signi®cantly inhibited with the PKC inhibitor, bisindolylmaleimide (BIM) (P50.05). 4 The role of lipoxygenase products in the depolarization to anandamide was investigated in the presence of the lipoxygenase inhibitor, 5,8,11-Eicosatriynoic acid (ETI). Depolarization to anandamide and arachidonic acid was signi®cantly inhibited in the presence of ET1 (P50.05). However, in the absence of calcium depolarization to anandamide was not inhibited by ETI. 5 Using confocal microscopy we have demonstrated the presence of vanilloid receptors on both neuropeptide containing nerves and nerves that did not stain for sensory neuropeptides. 6 These results demonstrate that anandamide evokes depolarization of guinea-pig vagus nerve, following activation of vanilloid receptors, a component of which involves the generation of lipoxygenase products. Furthermore, these receptors are distributed in both neuropeptide and nonneuropeptide containing nerves.

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“…In contrast to PEA, oleoyl ethanolamide (OEA) inhibited anandamide uptake (Rakhshan et al ., 2000) and degradation (Karava et al ., 2001). Like anandamide, OEA has been implicated in the neural regulation of feeding behaviors (Rodriguez de Fonseca et al ., 2001) by acting on peripheral sensory fibers.…”

Section: Introductionmentioning

confidence: 99%

The expanding field of cannabimimetic and related lipid mediators

Bradshaw

Walker

2005

British J Pharmacology

146

141

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The discovery of the endogenous cannabimimetic lipid mediators, anandamide and 2-arachidonoyl glycerol, opened the door to the discovery of other endogenous lipid mediators similar in structure and function. The majority of these compounds do not bind appreciably to known cannabinoid receptors; yet some of them produce cannabimimetic effects while others exert actions through novel mechanisms that remain to be elucidated. This review explores the growing diversity of recently discovered putative lipid mediators and their relationship to the endogenous cannabinoid system. The possibility that there remain many unidentified signalling lipids coupled with the evidence that many of these yield bioactive metabolites due to actions of known enzymes (e.g. cyclooxygenases, lipoxygenases, cytochrome P450s) suggests the existence of a large and complex family of lipid mediators about which only little is known at this time. The elucidation of the biochemistry and pharmacology of these compounds may provide therapeutic targets for a variety of conditions including sleep dysfunction, eating disorders, cardiovascular disease, as well as inflammation and pain.

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Anandamide amidohydrolase activity, released in the medium by Tetrahymena pyriformis. Identification and partial characterization

Cited by 15 publications

References 45 publications

Evolutionary origins of the endocannabinoid system

Evolutionary origins of the endocannabinoid system

Characterization of the anandamide induced depolarization of guinea‐pig isolated vagus nerve

The expanding field of cannabimimetic and related lipid mediators

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