Analytical validation of the Sysmex XT‐2000iV for cell counts in canine and feline effusions and concordance with cytologic diagnosis

Cunha, Nazaré Pinto da; Giordano, Alessia; Caniatti, M.; Paltrinieri, Saverio

doi:10.1111/j.1939-165x.2008.00107.x

Cited by 20 publications

(24 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The use of hematology analyzers using flow cytometry for differential counting on “non‐blood” fluid samples has been investigated in both laboratory animals and in dogs and cats . The results of rodent BAL fluid differential leukocyte counts performed by the ADVIA 120 and Sysmex XT‐2000iV compare favorably to 200‐cell manual differential counts, although adjustment of the software is required for BAL analysis .…”

Section: Discussionmentioning

confidence: 99%

“…The results of rodent BAL fluid differential leukocyte counts performed by the ADVIA 120 and Sysmex XT‐2000iV compare favorably to 200‐cell manual differential counts, although adjustment of the software is required for BAL analysis . These analyzers have also been useful in providing a preliminary cytologic diagnosis for canine and feline abdominal, thoracic, and pericardial fluids . Digital microscopy is another technique under investigation in human fluid cytology.…”

Section: Discussionmentioning

confidence: 99%

“…30,31 These analyzers have also been useful in providing a preliminary cytologic diagnosis for canine and feline abdominal, thoracic, and pericardial fluids. 32,33 Digital microscopy is another technique under investigation in human fluid cytology. For example, one study reported good to excellent correlation between automated digital and manual 200-cell differential counts on cerebrospinal fluid, abdominal fluid, and peritoneal dialysis fluid.…”

Section: Lai Categorymentioning

confidence: 99%

See 2 more Smart Citations

Reliability of 400‐cell and 5‐field leukocyte differential counts for equine bronchoalveolar lavage fluid

Fernandez

Hecker

Gilroy

et al. 2013

Veterinary Clinical Pathol

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Lai Categorymentioning

confidence: 99%

See 1 more Smart Citation

Reliability of 400‐cell and 5‐field leukocyte differential counts for equine bronchoalveolar lavage fluid

Fernandez

Hecker

Gilroy

et al. 2013

Veterinary Clinical Pathol

View full text Add to dashboard Cite

show abstract

“…1 The Sysmex XT-2000iV™ (hereafter, Sysmex analyzer), c a modification of the human XT-2000i system equipped with veterinary software, compared favorably to the Cell-Dyn 3500 10,11,26 and has been considered suitable for the measurement of canine body cavity fluids. 18 However, a comparison with the ADVIA 2120 has not been made. The impact of sample storage on the results and the morphology of cytograms are of great importance, especially for commercial laboratories that are commonly dealing with preanalytic errors such as the use of an incorrect anticoagulant or sample aging.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the automated hematology analyzer Sysmex XT-2000iV™ compared to the ADVIA® 2120 for its use in dogs, cats, and horses. Part II

et al. 2012

View full text Add to dashboard Cite

Abstract. The automated laser-based hematology analyzer Sysmex XT-2000iV™ provides a 5-part differential count and specific cytograms that are of great interest for large veterinary laboratories. The aim of the study was to validate the Sysmex XT-2000iV compared to the laser-based hematology analyzer ADVIA® 2120 and manual differential in dogs, cats, and horses as well as the impact of anticoagulant (heparin, ethylenediamine tetra-acetic acid [EDTA], and citrate) and storage at 22°C and 4°C. Consecutive fresh K 3 -EDTA blood samples from 216 cats, 314 dogs, and 174 horses were included. The impact of anticoagulant and sample storage was assessed in specimens obtained from an additional 9 cats, 10 dogs, and 10 horses. Agreement between both analyzers was excellent to good except for monocytes and canine reticulocytes. Spearman rank correlation coefficients (r s ) between Sysmex XT-2000iV and manual differential were good to fair and ranged from 0.91 (cat lymphocytes) to 0.44 (cat monocytes). Hematocrit value (Hct), mean corpuscular hemoglobin (MCH), MCH concentration (MCHC; all: P < 0.001), and mean corpuscular volume (MCV; P < 0.01) were higher in canine citrated blood compared to heparin and EDTA. In cats, lymphocytes and monocytes were lower in heparinized blood compared to EDTA (P < 0.05), whereas in horses no significant effect was seen. Regarding storage time and temperature, white and red blood cell counts, hemoglobin, and MCH were stable. Hct, MCV, and MCHC were influenced by erythrocyte swelling. Differential count remained stable for 24 hr (22°C) and nearly 72 hr (4°C) except for monocytes. The overall performance of the Sysmex XT2000iV was excellent and compared favorably with that of the ADVIA 2120. A special strength was the excellent detection of feline eosinophils.

show abstract

“…In 8 of these cases, the WBC-DIFF count was lower than in the case of the fresh sample. Conversely, in 6 of 27 cases (cats 5,9,11,12,14,26), the modified ΔTNC was higher than the original, and in 3 of these cases the WBC-DIFF count was higher than that of the fresh sample. In 9 of 27 cases (cats 8,…”

Section: Brief Communicationmentioning

confidence: 87%

Diagnosing feline infectious peritonitis using the Sysmex XT-2000iV based on frozen supernatants from cavitary effusions

2017

Self Cite

View full text Add to dashboard Cite

The delta total nucleated cells (ΔTNC) measurement with the Sysmex XT-2000iV (Sysmex Europe, Norderstedt, Germany) has high diagnostic accuracy on effusions in feline infectious peritonitis (FIP) cases, but the test can be performed only on fresh samples. We evaluated whether supernatants from effusions retain the ability to induce cell clumping and assessed the diagnostic accuracy of this modified ΔTNC method. Effusions were collected from FIP cats ( n = 19) and from cats with other diseases ( n = 15). ΔTNC was measured on fresh samples and on frozen-thawed supernatants after the addition of feline blood at 1:10 dilution. Diagnostic accuracy was assessed at the cutoffs of suggestive of FIP (ΔTNC = 1.7) and consistent with FIP (ΔTNC = 3.4). The influence of the protein content, number of added cells, and magnitude of dilution were also investigated. Specificity and positive predictive value were 100% for both the methods. Sensitivity and negative predictive value were higher for the modified ΔTNC (84.2% and 83.3%, respectively, at the cutoff of 1.7; 78.9% and 78.9%, respectively, at the cutoff of 3.4) than for the ΔTNC on fresh samples (78.6% and 81.3%, respectively, at the cutoff of 1.7; 57.1% and 68.4%, respectively, at the cutoff of 3.4). Protein content, total cell count of the added blood, and magnitude of dilutions did not influence the results. Supernatants of frozen effusions from FIP cats retain the ability to induce cell clumping, thus the modified ΔTNC measurement is a reliable tool to diagnose FIP on samples that cannot be analyzed immediately.

show abstract

Analytical validation of the Sysmex XT‐2000iV for cell counts in canine and feline effusions and concordance with cytologic diagnosis

Cited by 20 publications

References 15 publications

Reliability of 400‐cell and 5‐field leukocyte differential counts for equine bronchoalveolar lavage fluid

Reliability of 400‐cell and 5‐field leukocyte differential counts for equine bronchoalveolar lavage fluid

Evaluation of the automated hematology analyzer Sysmex XT-2000iV™ compared to the ADVIA® 2120 for its use in dogs, cats, and horses. Part II

Diagnosing feline infectious peritonitis using the Sysmex XT-2000iV based on frozen supernatants from cavitary effusions

Contact Info

Product

Resources

About