Analytical Methods for Lipases Activity Determination: A Review

Stoytcheva, Margarita; Montero, Gisela; Zlatev, Roumen; León, José Á.; Gochev, Velizar

doi:10.2174/157341112801264879

Cited by 88 publications

(60 citation statements)

References 103 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Stoytcheva et al, (2010) recorded that the maximum lipolytic activity was 12 U/ml in the presence of olive oil by P. aurantiogriseum. A. niger produced lipase in a lipid free medium but required an inducer for improved production [40]. …”

Section: Effect Of Lipid Carbon Sources and Glucosementioning

confidence: 99%

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by <i>Aspergillus niger</i>

El-Batal

Farrag

Elsayed

et al. 2016

ILNS

View full text Add to dashboard Cite

Abstract. The present investigation was carried out to evaluate the effect of different growth conditions on lipase production by Aspegillus niger. The extracellular lipase producing fungus was isolated from spent bleaching earths. Optimizations of physical and chemical parameters were done for maximum lipase production using this isolate. Growth of the organism and lipase production were measured usig varying pH (4 -9), incubation temperature (20 -30 °C), incubation time (8 -80 hrs.), carbon sources, nitrogen sources, and shaking speed. Enhanced lipase production was observed at 24 °C, pH 7 and after 72hrs of incubation. Olive oil 5 % was observed as the most effective carbon source and Yeast extract 1.0 % as the most effective nitrogen source for lipase production. The optimum shaking value to get maximum lipase activity by Aspergillus niger was 200 rpm.

show abstract

Section: Effect Of Lipid Carbon Sources and Glucosementioning

confidence: 99%

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by <i>Aspergillus niger</i>

El-Batal

Farrag

Elsayed

et al. 2016

ILNS

View full text Add to dashboard Cite

show abstract

“…The enzymatic activity was measured through the volumetric method, which is based on the titrimetric determination of the free acids released from triacylglycerols by lipase catalyzed hydrolysis [12,13]. About 5 ml of olive oil emulsion, water and arabic gum (7%), 2 ml of phosphate buffer pH 7.0 (0.1M) and 10 mg of enzyme were incubated for one hour in a shaker incubator (TE-421, Tecnal, Piracicaba-SP/Brazil) at 40 °C and 140 orbitals per minute (OPM).…”

Section: Lipase Activitymentioning

confidence: 99%

“…According to these authors, at moderate temperatures the rate-limiting step for the irreversible heat deactivation of enzymes is the formation of an unfolded enzyme state (ܷ), as the reaction of Equation 12:…”

Section: Data Onmentioning

confidence: 99%

Activity of immobilized lipase from Candida antarctica (Lipozyme 435) and its performance on the esterification of oleic acid in supercritical carbon dioxide

Santos

Rezende

Martínez

2016

The Journal of Supercritical Fluids

View full text Add to dashboard Cite

“…The method is specific for fatty acids and more sensitive in comparison to titrimetric method [29,30]. Several authors …”

Section: Lipase Screening Liquid Mediummentioning

confidence: 99%

Isolation and Identification of a Novel Lipase Producing Bacteria from Oil Spilled Soil

Veerapagu¹,

Narayanan²,

Jeya³

et al. 2014

IJIRSET

View full text Add to dashboard Cite

Lipases are glycerol ester hydrolases that catalyze the hydrolysis of triglycerides to free fatty acids and glycerol. Oil spilled soil samples collected from Dharmapuri and Salem districts were screened for a novel lipase producing bacteria by screening in Tributyrin agar plate and Rhodamine olive oil agar and lipase screening liquid medium. Among the two hundred isolates thirty two isolates exhibited high lipolytic activity on TBA plate assay and twenty isolate exhibited high lipolytic activity on ROA plate assay The isolate BLP 141 produces lipase more than 12 U/ml in the further lipase screening liquid medium was selected as a novel lipase producing bacteria and identified by morphological and biochemical characteristics as Pseudomonas. The 16S rRNA gene amplified by bacterial universal primer 27F and 1492R yielded 1478 bp was analysed by BLAST confirmed it as Pseudomonas gessardii and was submitted in GenBank with accession number KJ547711.

show abstract

Analytical Methods for Lipases Activity Determination: A Review

Cited by 88 publications

References 103 publications

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by <i>Aspergillus niger</i>

Effect of Environmental and Nutritional Parameters on the Extracellular Lipase Production by <i>Aspergillus niger</i>

Activity of immobilized lipase from Candida antarctica (Lipozyme 435) and its performance on the esterification of oleic acid in supercritical carbon dioxide

Isolation and Identification of a Novel Lipase Producing Bacteria from Oil Spilled Soil

Contact Info

Product

Resources

About