Isolation and Identification of a Novel Lipase Producing Bacteria from Oil Spilled Soil

Veerapagu,; Narayanan, Sankara; Jeya,; Alagendran,

doi:10.15680/ijirset.2014.0312050

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…The tributyrin agar plates contain 1% emulsified tributyrin (v/v), 0.3% yeast extract (w/v) 0.5% tryptone(w/v), 2% agar, and pH was adjusted to 7. After incubation period, the presence of clear zone around the colonies indicated lipase production, each colony showing clear zones around growth were picked, and maintained on LB-agar plates for subsequent analysis (9).…”

Section: Isolation Of Lipolytic Bacterial Strainsmentioning

confidence: 99%

“…The production of bacterial lipase by tributyrin agar method was done. Among the two hundred isolates screened for lipolytic activity, thirty two isolates exhibited high lipolytic activity ( > 50 mm), ten isolate showed moderate activity ( 25 to below 50 mm), fifty three isolates showed low lipolytic activity (< 25 mm) and one hundred and five isolates have no lipolytic activity (9). Bacterial Lipase producers were isolated from oil spilled soil samples.Among the 200 isolates 20 shows clear zone in the tributyrin medium.…”

Section: -Effect Of Phsmentioning

confidence: 99%

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Isolation and optimization of thermophilic lipase producing bacteria from soil contaminated with used engines oil

Saad Hussein Khudhair

2022

JWSM

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Thirty two of thermophilic bacterial isolates producing lipase were selectively isolated from soil samples contaminated with used engines oil using Tributyrin agar medium. The qualitative assay of lipase activity was done on Tributyrin agar plates by measuring diameter of the clear zone. The isolates 6T, 14T and 29T of the isolated strains exhibited a greater zone of clearance than the others, indicating higher lipase activity. Secondary screening for lipase production was done using liquid basal medium with 1% (w/v) olive oil and pH 7. Results from quantitative assay showed that 6T, 14T and 29T isolates appeared highest ability to produce lipase enzyme 4 U/ml, 10 U/ml and 7 U/mlrespectively. Therefore, the 14T isolate was selected and identified based on their morphological and physiological characteristics which was subsequently identified and designated as Bacillus licheniformis. The effect of incubation time, temperature and pH influencing lipase production from Bacillus licheniformis14T isolate was determine. Results showed that the maximal lipase production was 11 U\ml after 3 day of incubation at medium pH7 and temperature 50 0C.

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Section: Isolation Of Lipolytic Bacterial Strainsmentioning

confidence: 99%

Section: -Effect Of Phsmentioning

confidence: 99%

Isolation and optimization of thermophilic lipase producing bacteria from soil contaminated with used engines oil

Saad Hussein Khudhair

2022

JWSM

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Screening, partial purification and characterization of the hyper-thermophilic lipase produced by a new isolate of Bacillus subtilis LP2

Yasar

Gülhan

Guduk

et al. 2020

Biocatalysis and Biotransformation

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Screening and molecular identification of lipolytic bacteria from spent bleaching earth

Soleha

Retnaningrum

2020

Biodiversitas

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Abstract. Soleha S, Retnaningrum E. 2020. Screening and molecular identification of lipolytic bacteria from spent bleaching earth. Biodiversitas 21: 4155-4161. Lipolytic bacteria can produce nanobioremediation materials that encourage researchers to explore the diversity of these bacteria. Lipolytic bacteria were isolated from spent bleaching earth (SBE). Bacteria isolates were screened for their lipolytic index and crude lipase activity. Strain with the highest lipolytic index and crude lipase activity were identified using the 16S rRNA gene as genetic markers. The maximum likelihood method in MEGA6 software has been used to analyze the relationship of the isolate. The results showed that strain SBE01 had the highest lipolytic index and crude lipase activity of 8.8 ± 0.7 mm and 5.7 U/mL, respectively. Based on 16S rRNA gene analysis, the isolate was identified as Moraxella sp. Phylogenetic tree was shown that SBE01 isolate was closely related to Moraxella osloensis. Moraxella sp. has the potential to be used as a bioremediation agent from oil-contaminated environment.

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Isolation and Identification of a Novel Lipase Producing Bacteria from Oil Spilled Soil

Cited by 3 publications

References 29 publications

Isolation and optimization of thermophilic lipase producing bacteria from soil contaminated with used engines oil

Isolation and optimization of thermophilic lipase producing bacteria from soil contaminated with used engines oil

Screening, partial purification and characterization of the hyper-thermophilic lipase produced by a new isolate of Bacillus subtilis LP2

Screening and molecular identification of lipolytic bacteria from spent bleaching earth

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