Analytical Grade Purification of Phycocyanin from Cyanobacteria

Khazi, Mahammed Ilyas; Demirel, Zeliha; Liaqat, Fakhra; Dalay, Meltem Conk

doi:10.1007/7651_2018_202

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…Phycocyanin has antioxidant, anticancer, liver and kidney protection effects ( Piovan et al, 2022 ), and has been widely used in the production of drugs and nutritional products in recent years. Khazi et al (2020) pretreated Cyanobacteria to obtain the crude extract of phycocyanin. Ammonium sulfate was added into the crude extract in sections, and the supernatant was removed by centrifugation after full stirring, then the phycocyanin was resuspended and dialyzed overnight to achieve further purification effect.…”

Section: Rough Fractionationmentioning

confidence: 99%

1Progress, applications, challenges and prospects of protein purification technology

Hou

Liu

et al. 2022

Front. Bioeng. Biotechnol.

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Protein is one of the most important biological macromolecules in life, which plays a vital role in cell growth, development, movement, heredity, reproduction and other life activities. High quality isolation and purification is an essential step in the study of the structure and function of target proteins. Therefore, the development of protein purification technologies has great theoretical and practical significance in exploring the laws of life activities and guiding production practice. Up to now, there is no forthcoming method to extract any proteins from a complex system, and the field of protein purification still faces significant opportunities and challenges. Conventional protein purification generally includes three steps: pretreatment, rough fractionation, and fine fractionation. Each of the steps will significantly affect the purity, yield and the activity of target proteins. The present review focuses on the principle and process of protein purification, recent advances, and the applications of these technologies in the life and health industry as well as their far-reaching impact, so as to promote the research of protein structure and function, drug development and precision medicine, and bring new insights to researchers in related fields.

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Section: Rough Fractionationmentioning

confidence: 99%

1Progress, applications, challenges and prospects of protein purification technology

Hou

Liu

et al. 2022

Front. Bioeng. Biotechnol.

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“…Afterward, ammonium sulphate precipitates were subjected to dialysis overnight against Na-phosphate buffer using dialysis tubing cellulose membrane (Molecular Weight Cut-Off = 14 kDa, Sigma Aldrich) (Demirel & Sukatar, 2019). The purity ratio of the dialyzed sample was determined using UV-Vis spectrophotometer (GE Healthcare Ultrospec 1100 pro) at 620 and 280 nm (Khazi et al, 2018). The purified phycocyanin was freeze dried and stored at −20°C in the dark.…”

Section: Extraction and Purification Proceduresmentioning

confidence: 99%

Microencapsulation of phycocyanin by spray‐drying method: Effect of process parameters and wall materials

İlter

Koç

Demirel

et al. 2022

Food Processing Preservation

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Spray‐drying method was used for the microencapsulation of the extracted, purified phycocyanin from Arthrospira platensis to increase its stability and usability as a food colorant. The effect of process conditions – wall material type, ratio, and inlet‐air temperature – on physical and chemical properties was investigated. The moisture content and water activity of phycocyanin microcapsules were found as 1.80–3.18% and 0.15–0.24, respectively. The product with the largest particle size was 19.44 μm. Size distribution values were in 1.87–6.05. Optimum process condition was determined as 34.4% maltodextrin, 65.6% whey protein isolate, and 167.6°C inlet temperature. Moisture content (2.35%), aw (0.18), Tg (58.25°C), D[4,3] (11.92 μm), size distribution (2.016), color properties (L*: 79.37, a*: −6.69, b*: −5.29), total phenolic content (12.14 mgGA/L), and microencapsulation efficiency (87.11%) of the phycocyanin microcapsules were also determined at optimum. Microencapsulation efficiency increased with the maltodextrin and whey protein isolate together; the conditions of process variables were effective in the encapsulation process. Novelty impact statement Phycocyanin was prepared in a double‐layer emulsion and dried in a spray dryer, thus preventing degradation caused by environmental factors. The physical and chemical properties of the final product were examined and modeled. The conditions required to obtain the microencapsulated phycocyanin with optimum properties were examined.

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“…Besides, phycocyanin has other biological activities, like anti-inflammatory, anti-cancer, anti-diabetes, neuroprotective and hepatoprotective effects (Romay et al, 2003;Jiang et al, 2017;Prabakaran et al, 2020). Thereby, phycocyanin nowadays is widely used in food, nutraceutical, cosmetics, and pharmaceutical industries (Manirafasha et al, 2016;Khazi et al, 2020). Presently, the commercial price of phycocyanin varies enormously depending on its purity.…”

Section: Introductionmentioning

confidence: 99%

Enhanced phycocyanin production of Arthrospira maxima by addition of mineral elements and polypeptides using response surface methodology

Yao

Huang

et al. 2022

Front. Mar. Sci.

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IntroductionAs a pigment protein, phycocyanin has been widely used in the fields of food, nutraceutical and biotechnology due to its excellent biological activities of antioxidant. So far, Arthrospira has been generally considered as a great species for phycocyanin production.MethodsIn this study, independent and interactive effect of three factors, NaCl, Bainengsi (BS) and Bainengtai (BT) on microalgae growth and phycocyanin production were explored by response surface methodology (RSM). Using Box-Behnken design (BBD) method, the well-fitting quadratic models were established based on experimental results.ResultsMoreover, the maximum phycocyanin concentration reached 704.66 mg L-1 at the optimal condition (185 mM NaCl, 200 mg L-1 BS, and 20 mg L-1 BT), while the maximum phycocyanin content of 19.03% was obtained at another optimal condition (136 mM NaCl, 200 mg L-1 BS, and 50 mg L-1 BT). Compared to control, the concentration and content of phycocyanin were increased by 22.98% and 16.73%, respectively.DiscussionOverall, this study demonstrated that addition of exogenous substances (BS, BT) into culture medium optimized by RSM was an effective approach to increase phycocyanin production, which paved a potential way to realizing high efficient production of algal biomass and bioactive substances.

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Analytical Grade Purification of Phycocyanin from Cyanobacteria

Cited by 5 publications

References 15 publications

1Progress, applications, challenges and prospects of protein purification technology

1Progress, applications, challenges and prospects of protein purification technology

Microencapsulation of phycocyanin by spray‐drying method: Effect of process parameters and wall materials

Enhanced phycocyanin production of Arthrospira maxima by addition of mineral elements and polypeptides using response surface methodology

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