Enhanced phycocyanin production of Arthrospira maxima by addition of mineral elements and polypeptides using response surface methodology

Yao, Ting; Huang, Jianke; Su, Bocheng; Liang, Wei; Zhang, Aihua; Zhang, Dao‐Feng; Zhou, Yongsheng; Ma, Guiping

doi:10.3389/fmars.2022.1057201

Cited by 3 publications

(1 citation statement)

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“…The content of photosynthetic units of cells in relation to their pigment composition differs between different species of microalgae, and for this reason, growers have selected certain species known to overproduce some of them [19]. Well-known cases are the species of the genus Dunaliella for the production of β-carotene [21,22] and Arthrospira (Spirulina) for phycocyanin [23,24]. However, even for species selected for cultivation, there is much scope for optimizing and maximizing their pigment content, depending on how some critical conditions for cultivation are modified.…”

Section: Introductionmentioning

confidence: 99%

Quantity and Quality of Light on Growth and Pigment Content of Dunaliella sp. and Anabaena sp. Cultures and the Use of Their Absorption Spectra as a Proxy Method for Assessment

Hotos¹

2023

Preprint

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(1) Background: Microalgae cultures are greatly facilitated if growers can easily and economically ascertain the quantitative and qualitative status of the culture continuously with satisfactory ac-curacy. (2) Methods: The locally isolated microalgae Dunaliella sp. and Anabaena sp. were cultured in small volumes with 2 intensities of white light (2000 and 8000 lux) and with green, blue and red light and the increase of their biomass and pigments was studied. Pigment analyses, continuous recordings of absorption spectra and calibration curves were performed. (3) Results: The intensity of 8000 lux white light yielded the highest increase in biomass, chlorophylls and carotenoids in Dunaliella sp. while 2000 lux and green light caused the greatest increase in biomass and phy-cocyanin in Anabaena sp. From the examination of the absorption spectra, the evolution of the pigment content can be estimated and both pigments and biomass are correlated very satisfactorily with the wavelength of 750 nm. (4) Conclusions: The use of absorption spectra as an easy, fast and economical method can be a useful tool for a good approximation of the state of the microalgae culture. This is clearly shown when the spectra of the culture under different lighting colors are compared which have a catalytic effect on the level of the pigments leading to the increase in carotenoids and phycocyanin of the green light.

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Section: Introductionmentioning

confidence: 99%

Quantity and Quality of Light on Growth and Pigment Content of Dunaliella sp. and Anabaena sp. Cultures and the Use of Their Absorption Spectra as a Proxy Method for Assessment

Hotos¹

2023

Preprint

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show abstract

Quantity and Quality of Light on Growth and Pigment Content of Dunaliella sp. and Anabaena sp. Cultures and the Use of Their Absorption Spectra as a Proxy Method for Assessment

Hotos

2023

JMSE

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(1) Background: As microalgae cultures are affected by the quantity and quality of light, I explored this for two species. Additionally, I introduced a novel easy and economical way for the growers to easily and economically ascertain continuously with satisfactory accuracy the quantitative and qualitative status of their culture using absorption spectra. (2) Methods: The locally isolated chlorophyte Dunaliella sp. and the cyanobacterium Anabaena sp. were cultured in small volumes with two intensities of white light (2000 and 8000 lux) and with green, blue and red light, and the increase in their biomass and pigments was studied. Pigment analyses, continuous recordings of absorption spectra and calibration curves were used. (3) Results: The intensity of 8000 lux of white light yielded the highest increase in biomass, chlorophylls and carotenoids in Dunaliella sp., and the same was found for green and blue light, while 2000 lux and green light caused the greatest increase in biomass and phycocyanin in Anabaena sp. From the absorption spectra, the evolution of the pigment content can be estimated, and both pigments and biomass are correlated very strongly with those extracted from the spectra absorption of 750 nm. (4) Conclusions: The use of absorption spectra as an easy, fast and economical method can be a useful tool for a good approximation of the state of the microalgae culture. This is clearly shown when the spectra of the cultures under different light intensities and colors are compared having a catalytic effect on the level of the pigments leading to the increase in carotenoids and phycocyanin of the green light.

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Maximizing Polysaccharides and Phycoerythrin in Porphyridium purpureum via the Addition of Exogenous Compounds: A Response-Surface-Methodology Approach

Yi,

Zhang,

Huang

et al. 2024

Marine Drugs

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Phycoerythrin and polysaccharides have significant commercial value in medicine, cosmetics, and food industries due to their excellent bioactive functions. To maximize the production of biomass, phycoerythrin, and polysaccharides in Porphyridium purpureum, culture media were supplemented with calcium gluconate (CG), magnesium gluconate (MG) and polypeptides (BT), and their optimal amounts were determined using the response surface methodology (RSM) based on three single-factor experiments. The optimal concentrations of CG, MG, and BT were determined to be 4, 12, and 2 g L−1, respectively. The RSM-based models indicated that biomass and phycoerythrin production were significantly affected only by MG and BT, respectively. However, polysaccharide production was significantly affected by the interactions between CG and BT and those between MG and BT, with no significant effect from BT alone. Using the optimized culture conditions, the maximum biomass (5.97 g L−1), phycoerythrin (102.95 mg L−1), and polysaccharide (1.42 g L−1) concentrations met and even surpassed the model-predicted maximums. After optimization, biomass, phycoerythrin, and polysaccharides concentrations increased by 132.3%, 27.97%, and 136.67%, respectively, compared to the control. Overall, this study establishes a strong foundation for the highly efficient production of phycoerythrin and polysaccharides using P. purpureum.

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Enhanced phycocyanin production of Arthrospira maxima by addition of mineral elements and polypeptides using response surface methodology

Cited by 3 publications

References 57 publications

Quantity and Quality of Light on Growth and Pigment Content of <em>Dunaliella</em> sp. and <em>Anabaena</em> sp. Cultures and the Use of Their Absorption Spectra as a Proxy Method for Assessment

Quantity and Quality of Light on Growth and Pigment Content of <em>Dunaliella</em> sp. and <em>Anabaena</em> sp. Cultures and the Use of Their Absorption Spectra as a Proxy Method for Assessment

Quantity and Quality of Light on Growth and Pigment Content of Dunaliella sp. and Anabaena sp. Cultures and the Use of Their Absorption Spectra as a Proxy Method for Assessment

Maximizing Polysaccharides and Phycoerythrin in Porphyridium purpureum via the Addition of Exogenous Compounds: A Response-Surface-Methodology Approach

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