Analytical Determination and Electrochemical Characterization of the Oxazolidinone Antibiotic Linezolid

Abstract

Linezolid belongs to a class of antibiotics known as oxazolidinones. It is receiving great attention due to its activity toward multidrugs resistant bacteria, and simple and cheap methods for the analysis of this compound in pharmaceutical preparations are required. In this work, the electrochemistry of the antibiotic linezolid was investigated and a differential pulse voltammetric method based on the oxidation of the oxazolidinonic compound at a glassy carbon electrode has been developed and optimized for its determination; LOD of 50 mg L À1 and a linearity up to 200 mg L À1 were reported. The electrochemical process was studied by using different electrochemical techniques and confirmed by HPLC-MS/MS.

A Detailed Study of the (Electro)chemical Behavior of Bis(trifluoromethanesulfonyl)imide Based Ionic Liquids at Different Purification Steps

“…Passerini and co‐workers described the use of activated charcoal and alumina in the purification of ILs, involving multistep procedures 28 as well as a one‐pot purification (IL/ethyl acetate/alumina/active carbon mixture stirred at 60 °C) 29. In the present work a systematic investigation on inorganic and organic contaminants present at different level in three synthesized NTf 2 ‐based ionic liquids widely used for electrochemical applications 30 was pursued, namely N ‐butyl‐ N ‐methylpyrrolidinium‐bis(trifluoromethylsulfonyl)imide ([Py 1,4 ][NTf 2 ]), N ‐methoxyethyl‐ N ‐methylpyrrolidinium‐bis(trifluoromethylsulfonyl)imide ([Py 1,1O2 ][NTf 2 ]), and 1‐methyl‐3‐butylimidazolium‐bis(trifluoromethylsulfonyl)imide ([bmim][NTf 2 ]) (Figure 1) whose presence seriously affects both spectrochemical (see Figure 2) and electrochemical profiles (see Supporting Information).…”

“…Before use, the GC electrode was abraded with successively finer grades alumina (from 1 µm to 0.05 µm), rinsed with 5 % nitric acid and water and cleaned in an ultrasonic bath to remove traces of alumina. Then the surface was electrochemically cleaned by CV in 0.5 M H 2 SO 4 (15 cycles, initial potential E i =0.0 mV , final potential E f =+1400 mV, scan speed v =200 mV s −1 ) 30. A perfectly cleaned and smooth surface is necessary in order to obtain low background current and reproducible results.…”

Analytical Determination of the Oxazolidinone Antibiotic Linezolid at a Pencil Graphite and Carbon Paste Electrodes

“…There are only a few detection studies investigating the electrochemical features of linezolid and using a variety of study electrodes. Merli et al . used glassy carbon electrodes for analysis of linezolid with the differential pulse voltammetry technique and obtained an LOD value of 50 μg L −1 .…”

Stability-indicating capillary zone electrophoresis assay for the analysis of linezolid in tablets

“…Analysis of LNZ and its degradation products are usually assayed by high-performance liquid chromatography (HPLC) and spectrometry (Erlich et al, 2001;Krishna-Reddy et al, 2002; Page 2 / 7 2007; Merli et al, 2011;La Marca et al, 2012;Cios, Kus, Szymura-Oleksiak, 2013;Chavda et al, 2016); however, this technique has important limitations such as the high cost of the analysis and the excessive generation of residues due to the use of organic solvents. Capillary electrophoresis (CE) has also been described as an alternative to HPLC for LNZ assays.…”