Analytical bioconjugates, aptamers, enable specific quantitative detection of Listeria monocytogenes

Lee, Sang‐Hee; Ahn, Ji‐Young; Lee, Kyeong-Ah; Um, Hyun-Ju; Sekhon, Simranjeet Singh; Park, Tae Sun; Min, Jiho; Kim, Yang‐Hoon

doi:10.1016/j.bios.2015.01.009

Cited by 75 publications

(43 citation statements)

References 35 publications

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“…a good example expected properties of an affinity biosensor is narrated from Tsci et al 59 who indicated that efficiency, accuracy and high sensitive performance for dust mite allergen detection are some of the advantages conferred by the use of biosensors over other type of biomonitoring. Recently Lee et al 60 have investigated aptamer biosensor for whole live bacteria, allowing for a "quantitative" detection using a specif- Figure 4. illustrations of the aptamer-based sandwich assay (ABSA).…”

Section: Perspectivesmentioning

confidence: 99%

Toxicoproteomic approaches for analysis of microbial community inhabiting Asian dust particles

Sekhon

Ahn

Min

et al. 2015

Mol. Cell. Toxicol.

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in this review, we provide an overview of the bio-analytical approaches for proteomic analysis of asian dust storms. asian dust has been known to travel with high-speed eastward winds and affects air quality over Japan and even the western coast of the U.S. during severe occurrences. Several reports have shown that asian dust particles have a negative effect on a wide range of industries and human health activities. Here we give a short overview of asian dust feature and its proteomic analysis approaches including 2-De PaGe, metaproteomic analysis of large environmental samples, and automatic techniques for dust microbial analysis. Following this, we discuss the detection system of asian dust particles that can be integrated with biosensor platform.

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Section: Perspectivesmentioning

confidence: 99%

Toxicoproteomic approaches for analysis of microbial community inhabiting Asian dust particles

Sekhon

Ahn

Min

et al. 2015

Mol. Cell. Toxicol.

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“…56 Some studies were conducted for the generation of ssDNA aptamers for whole cells of L. monocytogenes . [57][58][59] Suh et al reported that ssDNA aptamers for L. monocytogenes were identified after six rounds of positive and two rounds of negative selection. 57 These ssDNA aptamers have binding affinity for L. monocytogenes with a micromolar K d value and only weak binding affinity for non-Listeria species.…”

Section: S Aureusmentioning

confidence: 99%

“…58 Lee et al adopted a whole-cell SELEX strategy, generated ssDNA aptamers LMCA2 and LMCA26, and then developed an aptamer-based sandwich assay platform for the quantification of L. monocytogenes . 59 They demonstrated that this platform yields a linear response over a wide concentration range of L. monocytogenes from 20 to 2 × 10 6 CFU/mL, thereby facilitating reliable detection of these bacteria at extremely low concentrations. 59…”

Section: S Aureusmentioning

confidence: 99%

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Oligonucleotide aptamers: promising and powerful diagnostic and therapeutic tools for infectious diseases

Pan

Liu

Zhang

2018

Journal of Infection

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The entire human population is at risk of infectious diseases worldwide. Thus far, the diagnosis and treatment of human infectious diseases at the molecular and nanoscale levels have been extremely challenging tasks because of the lack of effective probes to identify and recognize biomarkers of pathogens. Oligonucleotide aptamers are a class of small nucleic acid ligands that are composed of single-stranded DNA (ssDNA) or RNA and act as affinity probes or molecular recognition elements for a variety of targets. These aptamers have an exciting potential for diagnose and/or treatment of specific diseases. In this review, we highlight areas where aptamers have been developed as diagnostic and therapeutic agents for both bacterial and viral infectious diseases as well as aptamer-based detection.

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“…were used for aptamer magnetic capture before qPCR assay (Suh & Jaykus, 2013). An aptamer-based sandwich assay was also developed for L. monocytogenes with high sensitivity (Lee et al, 2015). However, the cross-reactivity with different serotypes of L. monocytogenes was unknown, since only one L. monocytogenes strain was used during the selection process.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticle-based paper sensor for multiple detection of 12 Listeria spp. by P60-mediated monoclonal antibody

Wang

Liu

Song

et al. 2016

Food and Agricultural Immunology

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The genus of Listeria consists of heterologous species and its presence in the food chain is an indicator of poor hygiene. However, a portable and simple paper sensor for detection of listeria spp. with high accuracy was still unknown. In this study, we prepared a pair of monoclonal antibodies (mAbs) that specifically recognize the P60 protein on the cell surface of Listeria spp. The selected pair was found to be sensitive to both the P60 protein and cell body of the genus Listeria. On this basis, a rapid paper sensor was established for sensitive Listeria spp. detection. The developed paper sensor broadly cross-reacted with the 12 tested strains of Listeria and the sensitivity in PBS buffer was 10 3-10 4 colonyforming units (CFU) judged by the gray values of the test line. No cross-reaction with any other gram-positive or gram-negative strains tested was observed. A study using milk samples showed that this paper sensor could detect samples contaminated with low levels of the tested Listeria spp. (1-9 CFU/mL) after 8 h of enrichment and further concentrate for approximately 10 times by centrifugation. The results were in accordance with those obtained using the polymerase chain reaction method.

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Analytical bioconjugates, aptamers, enable specific quantitative detection of Listeria monocytogenes

Cited by 75 publications

References 35 publications

Toxicoproteomic approaches for analysis of microbial community inhabiting Asian dust particles

Toxicoproteomic approaches for analysis of microbial community inhabiting Asian dust particles

Oligonucleotide aptamers: promising and powerful diagnostic and therapeutic tools for infectious diseases

Gold nanoparticle-based paper sensor for multiple detection of 12 Listeria spp. by P60-mediated monoclonal antibody

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