Analysis of Zinc in Biological Samples by Flame Atomic Absorption Spectrometry: Use of Addition Calibration Technique

Dutra, Rosilene Linhares; Cantos, Geny Aparecida; Carasek, Eduardo

doi:10.1385/bter:111:1:265

Cited by 12 publications

(8 citation statements)

References 19 publications

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“…[18] Although it was not significant, some other studies demonstrated lower serum zinc level in patient with AGA in comparison to normal individuals. [2223] At animal experimental models, zinc has an ambivalent but potent modulator of murine hair growth and in chemotherapy induced alopecia, zinc significantly accelerates the re-growth of normally pigmented hair shafts and promotion of hair follicles recovery. [9] Zinc has been shown to have an antiandrogenic potential and it specifically inhibits 5α-reductase type 1 and 2 activity, thus promoting hair growth.…”

Section: Discussionmentioning

confidence: 99%

Estimation of zinc and iron levels in the serum and hair of women with androgenetic alopecia: Case–control study

Dhaher

Yacoub

Jacob³

2018

Indian J Dermatol

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Background:Although female androgenetic alopecia (FAGA) occurs in genetically susceptible women, some trace elements may play an important role.Aim of the Study:This study aimed to evaluate serum and hair zinc and iron levels in patients with FAGA and to compare the findings with normal controls.Patients and Methods:A case–control study that was conducted at Dermatology Clinic in Basra General Hospital, Basra, Iraq. The participants were divided into two groups: The first group consisted of 27 women with FAGA and the second was age-matched 28 healthy women control group. The serum and hair zinc and iron assays were done for all participants.Results:Both hair and serum zinc levels in FAGA group were significantly lower than that in the control (103.4±25.5 ppm vs. 143.5±33.1 ppm for hair and 65.6±14.2 μg/dl vs. 128.4±41.4 μg/dl for serum). Hair iron level in FAGA was significantly lower than in control (17.9±3.8 ppm vs. 26.9±7.4 ppm, P>0.05). Serum iron level in FAGA group was lower than in the control, but it was not significant statistically (88.9±22.3 μg/dl vs. 100.9±18.9 μg/dl). Except for the hair iron, there was no significant correlation between zinc and iron concentrations in hair and serum with severity of alopecia.Conclusions:Zinc and iron levels in serum and hair were lower in FAGA compared to that of normal individuals indicating that trace elements might play an important role in the etiopathogenesis of FAGA.

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Section: Discussionmentioning

confidence: 99%

Estimation of zinc and iron levels in the serum and hair of women with androgenetic alopecia: Case–control study

Dhaher

Yacoub

Jacob³

2018

Indian J Dermatol

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“…Measurement of zinc levels in serum using the Atomic Absorption Spectroscopy (AAS) method for its specificity, sensitivity, accuracy and less cost. 18 We begin with making a standard zinc solution of 1000 mg zinc/liter by diluting 10 mL of nitric acid to 50 mL by adding 1,000 g of zinc, diluting it again to 100 ml. As much as 1 mL of standard zinc mixed with a 5/95 to 100 mL glycerol water solution, shaken 16 times, then 1, 2, 3, 4 mL were taken into 100 mL volume flask and added with a 5/95 glycerol/water solution.…”

Section: Serum Zinc Levelsmentioning

confidence: 99%

Zinc Supplementation in NFkB and Mucosal Intestinal Repair During LPS-Induced Sepsis in Rodent

Utomo¹,

Sudarmo²,

Sudiana³

2021

IJRP

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Background: Severe sepsis increases pro-inflammatory cytokines and damage to the intestinal mucosa, causing systemic translocation to the commensal bacteria. Low zinc levels were also found in patients with sepsis. Objective: This study aims to explain the mechanisms of sepsis improvement after zinc administration by measuring NFkB expression and mucosal intestinal repair. Material and Methods: Samples of 40 rats were randomized into 4 group of Control, LPS, LPS-Zinc, and Zinc . Blood sampling in 2-hour after LPS or placebo administration to measure zinc level. Zinc was administered in LPS-Zinc and Zinc group, placebo was given in other groups. Blood sampling in 8, 24 and 72 hours to measure NFkB monocyte expression by sandwich-ELISA method and in 72 hours also measured the zinc content by AAS method and jejunal tissue by SEM analysis. Statistical analyzes used were one-way Anova, Kruskall Wallis, Mann-Whitney, Paired-t tests, Wilcoxon Signed Rank Test and path analysis. Results: There were decreased in NFkB monocyte expression in the LPS-Zinc group compared to the LPS group. Conclusion: Improvement of intestinal mucosa occured in LPS-Zinc group. The administration of zinc improves the condition of sepsis and improving intestinal mucosa (villi structure of jejunum).

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“…Num indivíduo saudável a excreção de Zn através da urina deve situar-se entre 300-600 μg/L. 32 A concentração de Zn diminui com o avançar da idade, havendo acentuado decréscimo a partir dos 75 anos de idade. Esta diminuição em doentes de Alzheimer aumenta a patologia amiloide.…”

Section: Importância Dos Metais Em Doenças Neurodegenerativasunclassified

Especiação de cobre e zinco em urina: importância dos metais em doenças neurodegenerativas

Leal¹,

Catarino²,

Pimenta³

et al. 2012

Quím. Nova

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Recebido em 5/3/12; aceito em 19/6/12; publicado na web em 31/8/12 SPECIATION OF COPPER AND ZINC IN URINE -IMPORTANCE OF METALS IN NEURODEGENERATIVE DISEASES.Metals such as copper and zinc are essential for the development and maintenance of numerous enzymatic activities, mitochondrial functions, neurotransmission, and also for memorization and learning. However, disruption in their homeostasis can cause neurodegenerative disorders such as the Alzheimer and Parkinson diseases. In this work, the speciation of copper and zinc in urine samples was carried out. To this end, free and total metal concentrations were determined by square wave anodic stripping voltammetry using a glassy carbon electrode coated with bismuth film. The digestion of the samples was performed in a microwave with the addition of oxidant reagents.Keywords: metals; neurodegenerative diseases; stripping voltammetry. INTRODUÇÃO Alterações bioquímicas, fisiológicas e neurológicas em doenças neurodegenerativasAs doenças neurodegenerativas, que se caracterizam pela progressiva perda da estrutura e função neuronal, têm vindo a aumentar em todo o mundo, revelando-se na atualidade um grave problema humano, social e até económico. Apesar de ainda não serem conhecidos todos os processos bioquímicos e/ou fisiológicos que conduzem ao desenvolvimento destas desordens, sabe-se hoje que as principais doenças neurodegenerativas, tais como a doença de Alzheimer e a doença de Parkinson, apresentam algumas semelhanças, especialmente no que respeita à deposição intra ou extracelular de emaranhados proteicos. 1 A doença de Alzheimer é uma doença progressiva e irreversível, que começa por atingir a memória e com a evolução acaba por afetar outras funções mentais. Um doente de Alzheimer, após diagnosticada a doença, resiste em média 8 anos.2-4 A doença de Alzheimer pode surgir com uma causa genética ou de forma esporádica, apontando--se neste caso vários fatores responsáveis pelo seu aparecimento, tais como a alteração da transmissão colinérgica, a cascata amiloide, a isoforma 4 da apoliproteína E e a proteína tau.5-10 Na doença de Alzheimer, do ponto de vista histopatológico, verifica-se a deposição de placas senis extracelulares (agregados do péptido β-amiloide (βA)), bem como a deposição de fibrilas intracelulares de proteína tau polimerizada (hiperfosforilada).9 O péptido βA resulta da clivagem proteolítica da proteína precursora β-amiloide (PPA) por α-, β-e γ-secretases.11 As formas mais frequentes de βA são constituídas por 40 e 42 aminoácidos.9,12-14 Os βA formados que assumem maior comprimento têm mais facilidade para uma rápida agregação, oligomerização e formação de fibrilas. 15 A forma de oligómero é a mais neurotóxica. O mecanismo ativo existente para remover oligó-meros do βA ocorre pela promoção da sua desagregação enzimática em monómeros.9 Os axónios são constituídos em grande parte pela proteína tau e microtúbulos de baixo peso molecular. Os oligómeros formados do péptido βA provocam instabilidade na proteína tau, havendo uma rápida dissociação dos mi...

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Analysis of Zinc in Biological Samples by Flame Atomic Absorption Spectrometry: Use of Addition Calibration Technique

Cited by 12 publications

References 19 publications

Estimation of zinc and iron levels in the serum and hair of women with androgenetic alopecia: Case–control study

Estimation of zinc and iron levels in the serum and hair of women with androgenetic alopecia: Case–control study

Zinc Supplementation in NFkB and Mucosal Intestinal Repair During LPS-Induced Sepsis in Rodent

Especiação de cobre e zinco em urina: importância dos metais em doenças neurodegenerativas

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