Background: Severe sepsis increases pro-inflammatory cytokines, causing systemic translocation to the commensal bacteria. A study in Southeast Asia involving 13 public hospitals in 3 countries, including Indonesia, found that sepsis mortality in children was 2% and a 28% incidence of severe sepsis. Low plasma zinc levels were found in severe and critically septic patients in children and adults.Objective: This study aims to explain the mechanism of sepsis improvement after zinc administration through MDA and MMP8 during LPS-induced sepsis in rodents. Material and Methods: We divided samples of 40 rats into four groups of Control, LPS, LPS-Zinc, and Zinc. Blood sampling in 2-hour after LPS or placebo administration to measure zinc level. Zinc was administered in LPS-Zinc and Zinc group and placebo in two other groups. Blood sampling in 8, 24, and 72 hours to measure MDA and MMP-8 by sandwich-ELISA method. Statistical analysis was carried out with one-way ANOVA, Kruskal Wallis, Mann-Whitney, Paired t-tests, Wilcoxon Signed Rank Test, and path analysis. Results: Oral administration of zinc decreased the expression of monocyte MDA and MMP-8. The mechanism of zinc in the repair of sepsis through inhibition of MDA and MMP-8. Zinc supplementation can possibly reduce the the risk of child mortality in sepsis case. Conclusion: Administration of zinc in sepsis improves the condition of sepsis by decreasing pro-inflammatory cytokine and increasing anti-inflammatory cytokine
Background: Severe sepsis increases pro-inflammatory cytokines and damage to the intestinal mucosa, causing systemic translocation to the commensal bacteria. Low zinc levels were also found in patients with sepsis. Objective: This study aims to explain the mechanisms of sepsis improvement after zinc administration by measuring NFkB expression and mucosal intestinal repair. Material and Methods: Samples of 40 rats were randomized into 4 group of Control, LPS, LPS-Zinc, and Zinc . Blood sampling in 2-hour after LPS or placebo administration to measure zinc level. Zinc was administered in LPS-Zinc and Zinc group, placebo was given in other groups. Blood sampling in 8, 24 and 72 hours to measure NFkB monocyte expression by sandwich-ELISA method and in 72 hours also measured the zinc content by AAS method and jejunal tissue by SEM analysis. Statistical analyzes used were one-way Anova, Kruskall Wallis, Mann-Whitney, Paired-t tests, Wilcoxon Signed Rank Test and path analysis. Results: There were decreased in NFkB monocyte expression in the LPS-Zinc group compared to the LPS group. Conclusion: Improvement of intestinal mucosa occured in LPS-Zinc group. The administration of zinc improves the condition of sepsis and improving intestinal mucosa (villi structure of jejunum).
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