Analysis of the In Vivo and In Vitro Effects of Photodynamic Therapy on Breast Cancer by Using a Sensitizer, Sinoporphyrin Sodium

Abstract

Photodynamic therapy (PDT) is an emerging theranostic modality for various cancers and diseases. Photosensitizers are critical components for PDT. Sinoporphyrin sodium, referred to as DVDMS, is a newly identified photosensitizer that was isolated from Photofrin. Here, we evaluated the effects of DVDMS-mediated PDT (DVDMS-PDT) on tumor cell proliferation and metastasis in the highly metastatic 4T1 cell line and a mouse xenograft model. DVDMS-PDT elicited a potent phototoxic effect in vitro, which was abolished using the reactive oxygen species (ROS) scavenger N-acetylcysteine. In addition, DVDMS-PDT effectively inhibited the migration of 4T1 cells in scratch wound-healing and transwell assays. Using an in vivo mouse model, DVDMS-PDT greatly prolonged the survival time of tumor-bearing mice and inhibited tumor growth and lung metastasis, consistent with in vitro findings. PDT with DVDMS had a greater anti-tumor efficacy than clinically used Photofrin. Moreover, preliminary toxicological results indicate that DVDMS is relatively safe. These results suggest that DVDMS is a promising sensitizer that warrants further development for use in cancer treatment with PDT or other sensitizing agent-based therapies.

The antibacterial effect of sinoporphyrin sodium photodynamic therapy on Staphylococcus aureus planktonic and biofilm cultures

“…The structure and property of sensitizer greatly affect the efficiency of PDT. Our previous studies have shown good photosensitivity of DVDMS against various cancers . We speculate that DVDMS mediated PDT (DVDMS‐PDT) would also produce significant potential in PACT.…”

Förster Resonance Energy Transfer‐Activated Processes in Smart Nanotheranostics Fabricated in a Sustainable Manner

“…The prolonged 980 nm excitation resulted in an increasedp hototoxicity effect measured by proliferation MTT assay commonly applied to evaluate cell sensitivity or cell death after photodynamic protocols [61,62] (Figure5d). The prolonged 980 nm excitation resulted in an increasedp hototoxicity effect measured by proliferation MTT assay commonly applied to evaluate cell sensitivity or cell death after photodynamic protocols [61,62] (Figure5d).…”

Theranostic nanosensitizers for highly efficient MR/fluorescence imaging‐guided sonodynamic therapy of gliomas

“…On this ground, we herein report a multifunctional theranostic agent that integrates imaging and therapy functionalities into a single nano‐platform for highly efficient imaging‐guided SDT. Organic sinoporphyrin sodium (DVDMS) molecules were initially used as the sonosensitizer since they have been widely used in PDT and SDT for the therapy of many human tumours . Paramagnetic Mn centres were further anchored into DVDMS molecules (DVDMS‐Mn) by directly chelating manganese ion (Mn 2+ ) into DVDMS.…”

“…Organic sinoporphyrin sodium (DVDMS) molecules were initially used as the sonosensitizer since they have been widely used in PDT and SDT for the therapy of many human tumours. [28][29][30] Paramagnetic Mn centres were further anchored into DVDMS molecules (DVDMS-Mn) by directly chelating manganese ion (Mn 2+ ) into DVDMS. To improve the delivery efficiency, the multifunctional sonosensitizer DVDMS-Mn were further encapsulated into liposomes via a typical thin-film rehydration method to fabricate DVDMS-Mn-Liposomes (DVDMS-Mn-LPs).…”