The antibacterial effect of sinoporphyrin sodium photodynamic therapy on Staphylococcus aureus planktonic and biofilm cultures

Mai, Bingjie; Wang, Xiaobing; Liu, Quanhong; Leung, Albert Wingnang; Wang, Xinliang; Xu, Chuanshan; Wang, Pan

doi:10.1002/lsm.22468

Cited by 22 publications

(13 citation statements)

References 41 publications

(70 reference statements)

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“…25 Our previous study 31 demonstrated ROS in PACT treatment in antibacterial action. Figure 4 International Journal of Nanomedicine downloaded from https://www.dovepress.com/ by 34.210.69.67 on 11-May-2018 For personal use only.…”

Section: Discussionmentioning

confidence: 94%

“…Research has shown that DVDMS is a good sensitizer of antibacterial activity in vitro and can produce a large amount of ROS. 31 The clinical value of PACT as a topical antimicrobial treatment depends both on its bactericidal activity and its cytotoxicity toward host tissue. 18 In this study, we demonstrated that it is possible to photoinactivate S. aureus rapidly when present in a burn infected with DVDMS as PS in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

“…We observed that light-activated DVDMS enhanced intracellular ROS levels, which significantly induced cell death and markedly damaged S. aureus. 31 In this study we investigated in vivo PACT of second-degree thermal burn wounds in mice with S. aureus and MDR S. aureus infections ( Figure 1). We hypothesized that PACT could significantly downregulate inflammation of the infected tissues in the burns.…”

mentioning

confidence: 99%

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Photodynamic antimicrobial chemotherapy for Staphylococcus aureus and multidrug-resistant bacterial burn infection in vitro and in vivo

Mai¹,

Gao²,

Li³

et al. 2017

IJN

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Background and objectives Antibiotic resistance has emerged as one of the most important determinants of outcome in patients with serious infections, along with the virulence of the underlying pathogen. Photodynamic antimicrobial chemotherapy (PACT) has been proposed as an alternative approach for the inactivation of bacteria. This study aims to evaluate the antibacterial effect of sinoporphyrin sodium (DVDMS)-mediated PACT on Staphylococcus aureus and multidrug resistant S. aureus in vitro and in vivo. Materials and methods Bacteria were incubated with DVDMS and exposed to treatment with light. After PACT treatment, colony-forming units were counted to estimate the bactericidal effect. Intracellular reactive oxygen-species production was detected by flow cytometry. Flow cytometry and fluorescence-microscopy detection of bacterial cell-membrane permeability. Enzyme-linked immunosorbent assays were used to determine expression of VEGF, TGFβ 1 , TNFα, IL6, and bFGF factors in burn infection. Results DVDMS-PACT effectively killed bacterial proliferation. Intracellular ROS levels were enhanced obviously in the PACT-treatment group. SYTO 9 and propidium iodide staining showed a decrease in the ratio of green:red fluorescence intensity in the PACT-treatment group in comparison to the control group. Enzyme-linked immunosorbent-assay results revealed that in the healing process, the expression of bFGF, TGFβ 1 , and VEGF in the treatment group were higher than in the control group, which inhibited inflammation-factor secretion. In addition, skin-tissue bacteria were reduced after treatment. Conclusion These results indicate that DVDMS-PACT presents significant bactericidal activity and promotes wound healing after burn infections.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Photodynamic antimicrobial chemotherapy for Staphylococcus aureus and multidrug-resistant bacterial burn infection in vitro and in vivo

Mai¹,

Gao²,

Li³

et al. 2017

IJN

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“…Not only new species of multi-drug-resistant bacteria but also super bacteria have been continuously observed due to mutations that confer resistance against the number of antibiotics developed for the prevention and therapy of pathogenic bacterial diseases [ 1 , 2 ]. Staphylococcus aureus ( S. aureus ) is one of the pathogenic bacteria, which promotes infections by producing virulence factors and causes bacteremia, endocarditis, skin and soft tissue infections, as well as hospital-acquired infections.…”

Section: Introductionmentioning

confidence: 99%

Interdigitated and Wave-Shaped Electrode-Based Capacitance Sensor for Monitoring Antibiotic Effects

Park

Lee

Hwang

et al. 2020

Sensors

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Label-free and real-time monitoring of the bacterial viability is essential for the accurate and sensitive characterization of the antibiotic effects. In the present study, we investigated the feasibility of the interdigitated and wave-shaped electrode (IWE) for monitoring the effect of tetracycline or kanamycin on Staphylococcus aureus (S. aureus) and methicillin-resistant S.aureus (MRSA). The electrical impedance spectra of the IWE immersed in the culture media for bacterial growth were characterized in a frequency range of 10 Hz to 1 kHz. The capacitance index (CI) (capacitance change relevant with the bacterial viability) was used to monitor the antibiotic effects on the S. aureus and MRSA in comparison to the traditional methods (disk diffusion test and optical density (OD) measurement). The experimental results showed that the percentage of change in CI (PCI) for the antibiotic effect on MRSA was increased by 51.58% and 57.83% in kanamycin and control, respectively. In contrast, the PCI value decreased by 0.25% for tetracycline, decreased by 52.63% and 37.66% in the cases of tetracycline and kanamycin-treated S. aureus, and increased 2.79% in the control, respectively. This study demonstrated the feasibility of the IWE-based capacitance sensor for the label-free and real-time monitoring of the antibiotic effects on S.aureus and MRSA.

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“…The effect of PDT on S . aureus biofilm has been investigated with different photosensitizers, such as malachite green, methylene blue, and sinoporphyrin sodium [ 8 , 9 , 10 ]; however, the antimicrobial effect of ALA-PDT and its combined effect with other approaches on S . aureus biofilm is still extremely limited.…”

Section: Introductionmentioning

confidence: 99%

5-aminolevulinic acid-mediated photodynamic therapy and its strain-dependent combined effect with antibiotics on Staphylococcus aureus biofilm

Zhang

Zhao

et al. 2017

PLoS ONE

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Staphylococcus aureus (S. aureus) is hard to be eradicated, not only due to the emergence of antibiotic resistant strains but also because of its ability to form biofilm. Antibiotics are the major approach to treating biofilm infections, but their effects are unsatisfactory. One of the potential alternative treatments for controlling biofilm infections is photodynamic therapy (PDT), which requires the administration of photosensitizer, followed by light activation. 5-aminolevulinic acid (ALA), a natural photosensitizer prodrug, presents favorable characteristics, such as easy penetration and rapid clearance. These advantages enable ALA-based PDT (ALA-PDT) to be well-tolerated by patients and it can be repeatedly applied without cumulative toxicity or serious side effects. ALA-PDT has been proven to be an effective treatment for multidrug resistant pathogens; however, the study of its effect on S. aureus biofilm is limited. Here, we established our PDT system based on the utilization of ALA and a light-emitting diode, and we tested the effect of ALA-PDT on S. aureus biofilm as well as the combined effect of ALA-PDT and antibiotics on S. aureus biofilm. Our results showed that ALA-PDT has a strong antibacterial effect on S. aureus biofilm, which was confirmed by the confocal laser scanning microscope. We also found that lethal photosensitization occurred predominantly in the upper layer of the biofilm, while the residual live bacteria were located in the lower layer of the biofilm. In addition, the improved bactericidal effect was observed in the combined treatment group but in a strain-dependent manner. Our results suggest that ALA-PDT is a potential alternative approach for future clinical use to treat S. aureus biofilm-associated infections, and some patients may benefit from the combined treatment of ALA-PDT and antibiotics, but drug sensitivity testing should be performed in advance.

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The antibacterial effect of sinoporphyrin sodium photodynamic therapy on Staphylococcus aureus planktonic and biofilm cultures

Abstract: These results indicate DVDMS-PDT presents significant bactericidal activity.

Cited by 22 publications

References 41 publications

Photodynamic antimicrobial chemotherapy for <em>Staphylococcus aureus</em> and multidrug-resistant bacterial burn infection in vitro and in vivo

Photodynamic antimicrobial chemotherapy for <em>Staphylococcus aureus</em> and multidrug-resistant bacterial burn infection in vitro and in vivo

Interdigitated and Wave-Shaped Electrode-Based Capacitance Sensor for Monitoring Antibiotic Effects

5-aminolevulinic acid-mediated photodynamic therapy and its strain-dependent combined effect with antibiotics on Staphylococcus aureus biofilm

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