Analysis of tetracyclines from milk powder by molecularly imprinted solid‐phase dispersion based on a metal–organic framework followed by ultra high performance liquid chromatography with tandem mass spectrometry

Abstract

A novel molecularly imprinted polymer that could selectively recognize tetracyclines in milk powder was synthesized using a metal-organic framework as a support material, tetracycline as template molecule, and 3-aminophenylboronic acid as a functional monomer and a cross-linking agent. The novel molecularly imprinted polymer was characterized by Fourier transform infrared spectrometry, transmission electron microscopy, X-ray diffractometry, thermogravimetric analysis, and N adsorption/desorption measurements. The adsorption isotherms, adsorption kinetics, adsorption thermodynamics, and selective adsorption experiments of the novel molecularly imprinted polymer to tetracycline were also studied. The novel molecularly imprinted polymer was used as dispersant of matrix solid-phase dispersion to extraction tetracyclines. After that, the tetracyclines extracted from milk powder were determined by ultra high performance liquid chromatography with tandem mass spectrometry. Under the optimal conditions, the detection limits of tetracyclines were 0.217-0.318 ng/g. The relative standard deviations of intra- and interday precision ranged from 3.8 to 6.9% and from 2.8 to 7.4%, respectively. In all three concentration levels (1.0, 10, 50 ng/g), the recoveries of tetracyclines ranged from 84.7 to 93.9%. The method was successfully applied to the determination of tetracyclines in milk powder.

Electrospun metal‐organic framework/polyacrylonitrile composite nanofibrous mat as a microsorbent for the extraction of tetracycline residue in human blood plasma

“…A comparison study with other published work based on using SPE methods for the determination of TC in different matrices was conducted. As listed in Supporting Information Table S2, the LOD of the proposed method is better than most of the other methods.…”

Quantification of eight active ingredients in crude and processed radix polygoni multiflori applying miniaturized matrix solid‐phase dispersion microextraction followed by UHPLC

“…The chromatographic analyses were performed on a Waters ACQUITY UPLC system equipped with a photodiode array detector (Supporting Information Figure S2). The analytical column was an AQUITY UPLC BEH C 18…”

“…The matrix solid‐phase dispersion (MSPD) method is an extraction method first introduced by Barker in 1989, whose procedure combines sample disruption, extraction, fractionation, and purification within single procedure . Because of the rapidity, viability, flexibility, multifunctionality, and low cost of MSPD , various fields successfully employed MSPD, including large‐scale pesticide testing , biota fruits , milk power , cream samples , and Chinese herbs . In addition, miniaturized matrix solid‐phase dispersion (MMSPD), which requires less sample, elution reagent, and processing time in the whole procedure, has also proved to be an effective method for the extraction of inorganic iodine and iodinated amino acids and bioactive flavonoid glycosides from lime fruit .…”

Selective and sensitive determination of tetracyclines by HPLC with chemiluminescence detection based on a cerium(IV)‐methoxylated cypridina luciferin analogue system

“…Consequently, all of the parameters involved should be adequately studied. In prior studies, a mobile phase consisting of an organic solvent and a buffer solution, which was used to adjust the pH of eluents, has been reported for separation of TCAs [17][18][19][20][21][22][23]. The mobile phase with isocratic elution often consists of ACN combined with phosphoric acid [17], oxalic acid [18], formic acid [19,20], and acetic acid [21].…”